THRB Monoclonal Antibody (J52) | Invitrogen MA1-216 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

THRB Monoclonal Antibody (J52)

catalog :

MA1-216

quantity :

100 µL

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J52

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 31

Published Application/Species/Sample/Dilution	Reference
western blot; mouse	Liu Y, AYERS S, Milanesi A, Teng X, Rabi S, Akiba Y, et al. Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation. J Biol Chem. 2015;290:7402-15 pubmed publisher
western blot; rat; 1:1000	Lourbopoulos A, Mourouzis I, Karapanayiotides T, Nousiopoulou E, Chatzigeorgiou S, Mavridis T, et al. Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats. J Mol Neurosci. 2014;54:78-91 pubmed publisher
chromatin immunoprecipitation; human EMSA; human	AYERS S, Switnicki M, Angajala A, Lammel J, Arumanayagam A, Webb P. Genome-wide binding patterns of thyroid hormone receptor beta. PLoS ONE. 2014;9:e81186 pubmed publisher
western blot; human; 1:1000	Adamopoulos S, Gouziouta A, Mantzouratou P, Laoutaris I, Dritsas A, Cokkinos D, et al. Thyroid hormone signalling is altered in response to physical training in patients with end-stage heart failure and mechanical assist devices: potential physiological consequences?. Interact Cardiovasc Thorac Surg. 2013;17:664-8 pubmed publisher
chromatin immunoprecipitation; rat	Sharma P, Thakran S, Deng X, Elam M, Park E. Nuclear corepressors mediate the repression of phospholipase A2 group IIa gene transcription by thyroid hormone. J Biol Chem. 2013;288:16321-33 pubmed publisher
western blot; mouse; 1:1000	Mourouzis I, Kostakou E, Galanopoulos G, Mantzouratou P, Pantos C. Inhibition of thyroid hormone receptor ?1 impairs post-ischemic cardiac performance after myocardial infarction in mice. Mol Cell Biochem. 2013;379:97-105 pubmed publisher
western blot; human; 1:1000	Liappas A, Alexandros L, Mourouzis I, Iordanis M, Zisakis A, Athanasios Z, et al. Cell-type-dependent thyroid hormone effects on glioma tumor cell lines. J Thyroid Res. 2011;2011:856050 pubmed publisher
immunoprecipitation; human	Sadana P, Hwang J, Attia R, Arnold L, Neale G, Guy R. Similarities and differences between two modes of antagonism of the thyroid hormone receptor. ACS Chem Biol. 2011;6:1096-106 pubmed publisher
chromatin immunoprecipitation; rat	Attia R, Sharma P, Janssen R, Friedman J, Deng X, Lee J, et al. Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by CCAAT/enhancer-binding protein beta (C/EBPbeta). J Biol Chem. 2011;286:23799-807 pubmed publisher
western blot; rat; 1:1000	Kalofoutis C, Mourouzis I, Galanopoulos G, Dimopoulos A, Perimenis P, Spanou D, et al. Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction. Mol Cell Biochem. 2010;345:161-9 pubmed publisher
western blot; rat; 1:1000	Pantos C, Mourouzis I, Galanopoulos G, Gavra M, Perimenis P, Spanou D, et al. Thyroid hormone receptor alpha1 downregulation in postischemic heart failure progression: the potential role of tissue hypothyroidism. Horm Metab Res. 2010;42:718-24 pubmed publisher
western blot; mouse	Wang Y, Morimoto S, Du C, Lu Q, Zhan D, Tsutsumi T, et al. Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy. Cardiovasc Res. 2010;87:636-46 pubmed publisher
immunohistochemistry; rat; 1:10	László V, Dezso K, Baghy K, Papp V, Kovalszky I, Safrany G, et al. Triiodothyronine accelerates differentiation of rat liver progenitor cells into hepatocytes. Histochem Cell Biol. 2008;130:1005-14 pubmed publisher
western blot; rat; 1:1000	Pantos C, Xinaris C, Mourouzis I, Kokkinos A, Cokkinos D. TNF-alpha administration in neonatal cardiomyocytes is associated with differential expression of thyroid hormone receptors: a response prevented by T3. Horm Metab Res. 2008;40:731-4 pubmed publisher
western blot; rat; 1:1,000	Pantos C, Mourouzis I, Markakis K, Dimopoulos A, Xinaris C, Kokkinos A, et al. Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats. Eur J Cardiothorac Surg. 2007;32:333-9 pubmed
western blot; rat; 1:1,000	Pantos C, Mourouzis I, Xinaris C, Kokkinos A, Markakis K, Dimopoulos A, et al. Time-dependent changes in the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction: possible implications in cardiac remodelling. Eur J Endocrinol. 2007;156:415-24 pubmed
western blot; rat; 1:1000	Pantos C, Mourouzis I, Saranteas T, Paizis I, Xinaris C, Malliopoulou V, et al. Thyroid hormone receptors alpha1 and beta1 are downregulated in the post-infarcted rat heart: consequences on the response to ischaemia-reperfusion. Basic Res Cardiol. 2005;100:422-32 pubmed
	Ntari L, Mantzouratou P, Katsaouni A, Pantos C, Kollias G, Mourouzis I. Changes in Thyroid Hormone Signaling Mediate Cardiac Dysfunction in the Tg197 Mouse Model of Arthritis: Potential Therapeutic Implications. J Clin Med. 2021;10: pubmed publisher
	Custodio R, Kim M, Sayson L, Lee H, Ortiz D, Kim B, et al. Low striatal T3 is implicated in inattention and memory impairment in an ADHD mouse model overexpressing thyroid hormone-responsive protein. Commun Biol. 2021;4:1101 pubmed publisher
	Peng X, Zhang Y, Sun Y, Wang L, Song W, Li Q, et al. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells. Oncol Lett. 2018;16:785-792 pubmed publisher
	CARR F, Tai P, Barnum M, Gillis N, Evans K, Taber T, et al. Thyroid Hormone Receptor-? (TR?) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer. Endocrinology. 2016;157:3278-92 pubmed publisher
	Liu Y, Ando S, Xia X, Yao R, Kim M, Fondell J, et al. p62, A TFIIH subunit, directly interacts with thyroid hormone receptor and enhances T3-mediated transcription. Mol Endocrinol. 2005;19:879-84 pubmed
	Cao X, Kambe F, Moeller L, Refetoff S, Seo H. Thyroid hormone induces rapid activation of Akt/protein kinase B-mammalian target of rapamycin-p70S6K cascade through phosphatidylinositol 3-kinase in human fibroblasts. Mol Endocrinol. 2005;19:102-12 pubmed
	Maruvada P, Dmitrieva N, East Palmer J, Yen P. Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. Mol Biol Cell. 2004;15:1895-903 pubmed
	Siebler T, Robson H, Bromley M, Stevens D, Shalet S, Williams G. Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow. Bone. 2002;30:259-66 pubmed
	Rogatsky I, Zarember K, Yamamoto K. Factor recruitment and TIF2/GRIP1 corepressor activity at a collagenase-3 response element that mediates regulation by phorbol esters and hormones. EMBO J. 2001;20:6071-83 pubmed
	D Amati G, di Gioia C, Mentuccia D, Pistilli D, Proietti Pannunzi L, Miraldi F, et al. Increased expression of thyroid hormone receptor isoforms in end-stage human congestive heart failure. J Clin Endocrinol Metab. 2001;86:2080-4 pubmed
	Chen S, Shieh H, Lin J, Chang K, Lin K. Overexpression of thyroid hormone receptor beta1 is associated with thyrotropin receptor gene expression and proliferation in a human thyroid carcinoma cell line. J Endocrinol. 2000;165:379-89 pubmed
	Knipper M, Bandtlow C, Gestwa L, Köpschall I, Rohbock K, Wiechers B, et al. Thyroid hormone affects Schwann cell and oligodendrocyte gene expression at the glial transition zone of the VIIIth nerve prior to cochlea function. Development. 1998;125:3709-18 pubmed
	Palmer C, Hsu M, Griffin K, Raucy J, Johnson E. Peroxisome proliferator activated receptor-alpha expression in human liver. Mol Pharmacol. 1998;53:14-22 pubmed
	Meier C, Dickstein B, Ashizawa K, McClaskey J, Muchmore P, Ransom S, et al. Variable transcriptional activity and ligand binding of mutant beta 1 3,5,3'-triiodothyronine receptors from four families with generalized resistance to thyroid hormone. Mol Endocrinol. 1992;6:248-58 pubmed

image

image 1 :

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Thyroid Hormone Receptor beta-1 monoclonal antibody (Product # MA1-216) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

THRB Monoclonal Antibody (J52)

Catalog # :

MA1-216

Quantity :

100 µL

Pricing :

US 428

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Clone :

J52

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

Immunogen :

Purified human TR beta -1 protein.

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

c-erbA-2; c-erbAbeta; C-ERBA-BETA; ERBA 2; ERBA BETA; ERBA2; ERBA-BETA; fj24f03; GRTH; LOW QUALITY PROTEIN: thyroid hormone receptor beta; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; PRTH; RATT3REC; si:ch211-264a6.2; T3R[b]; T3Rbeta; T3rec; THR; THR1; thr2; Thrb; Thrb1; Thrb2; thyroid hormone nuclear receptor beta variant 1; thyroid hormone receptor beta; thyroid hormone receptor beta 1; thyroid hormone receptor beta 2; thyroid hormone receptor beta 3; thyroid hormone receptor beta1; thyroid hormone receptor beta-1; thyroid hormone receptor beta2; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR; TR beta; TR beta 1; TR[b]1; trb; TR-B1C-ERBA-2; TRbeta; TR-beta; TRbeta1; TRbeta2Delta; wu:fj24f03

more info or order :

Invitrogen product webpage