THRA/THRB Monoclonal Antibody (C3) | Invitrogen MA1-215 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

THRA/THRB Monoclonal Antibody (C3)

catalog :

MA1-215

quantity :

100 µL

price :

US 428

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
western blot; human	Privalsky M, Lee S, Hahm J, Young B, Fong R, Chan I. The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors. J Biol Chem. 2009;284:19554-63 pubmed publisher
western blot; Xenopus laevis; 1:1000	Saelim N, Holstein D, Chocron E, Camacho P, Lechleiter J. Inhibition of apoptotic potency by ligand stimulated thyroid hormone receptors located in mitochondria. Apoptosis. 2007;12:1781-94 pubmed
chromatin immunoprecipitation; mouse western blot; mouse; 1:1000	Nygard M, Becker N, Demeneix B, Pettersson K, Bondesson M. Thyroid hormone-mediated negative transcriptional regulation of Necdin expression. J Mol Endocrinol. 2006;36:517-30 pubmed
	Siddiq M, Hannila S, Zorina Y, Nikulina E, Rabinovich V, Hou J, et al. Extracellular histones, a new class of inhibitory molecules of CNS axonal regeneration. Brain Commun. 2021;3:fcab271 pubmed publisher
	Lauby S, McGowan P. Early life variations in temperature exposure affect the epigenetic regulation of the paraventricular nucleus in female rat pups. Proc Biol Sci. 2020;287:20201991 pubmed publisher
	Berner D, Hoja U, Zenkel M, Ross J, Uebe S, Paoli D, et al. The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome. Hum Mol Genet. 2019;: pubmed publisher
	Luan H, Mohapatra B, Bielecki T, Mushtaq I, Mirza S, Jennings T, et al. Loss of the Nuclear Pool of Ubiquitin Ligase CHIP/STUB1 in Breast Cancer Unleashes the MZF1-Cathepsin Pro-oncogenic Program. Cancer Res. 2018;78:2524-2535 pubmed publisher
	Matesanz N, Bernardo E, Acin Perez R, Manieri E, Pérez Sieira S, Hernández Cosido L, et al. MKK6 controls T3-mediated browning of white adipose tissue. Nat Commun. 2017;8:856 pubmed publisher
	Figliozzi R, Chen F, Hsia S. Reversing thyroid-hormone-mediated repression of a HSV-1 promoter via computationally guided mutagenesis. J Cell Sci. 2017;130:3740-3748 pubmed publisher
	Figliozzi R, Chen F, Balish M, Ajavon A, Hsia S. Thyroid hormone-dependent epigenetic suppression of herpes simplex virus-1 gene expression and viral replication in differentiated neuroendocrine cells. J Neurol Sci. 2014;346:164-73 pubmed publisher
	Harvey C, Bassett J, Maruvada P, Yen P, Williams G. The rat thyroid hormone receptor (TR) Deltabeta3 displays cell-, TR isoform-, and thyroid hormone response element-specific actions. Endocrinology. 2007;148:1764-73 pubmed
	Storey N, Gentile S, Ullah H, Russo A, Muessel M, Erxleben C, et al. Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone. Proc Natl Acad Sci U S A. 2006;103:5197-201 pubmed
	Goodson M, Jonas B, Privalsky M. Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors. J Biol Chem. 2005;280:7493-503 pubmed
	Saelim N, John L, Wu J, Park J, Bai Y, Camacho P, et al. Nontranscriptional modulation of intracellular Ca2+ signaling by ligand stimulated thyroid hormone receptor. J Cell Biol. 2004;167:915-24 pubmed
	Mengeling B, Pan F, Privalsky M. Novel mode of deoxyribonucleic acid recognition by thyroid hormone receptors: thyroid hormone receptor beta-isoforms can bind as trimers to natural response elements comprised of reiterated half-sites. Mol Endocrinol. 2005;19:35-51 pubmed
	Frankton S, Harvey C, Gleason L, Fadel A, Williams G. Multiple messenger ribonucleic acid variants regulate cell-specific expression of human thyroid hormone receptor beta1. Mol Endocrinol. 2004;18:1631-42 pubmed
	Nakano K, Matsushita A, Sasaki S, Misawa H, Nishiyama K, Kashiwabara Y, et al. Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells. Biochem J. 2004;378:549-57 pubmed
	Shibusawa N, Hashimoto K, Nikrodhanond A, Liberman M, Applebury M, Liao X, et al. Thyroid hormone action in the absence of thyroid hormone receptor DNA-binding in vivo. J Clin Invest. 2003;112:588-97 pubmed
	Shibusawa N, Hollenberg A, Wondisford F. Thyroid hormone receptor DNA binding is required for both positive and negative gene regulation. J Biol Chem. 2003;278:732-8 pubmed
	Osburn D, Shao G, Seidel H, Schulman I. Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions. Mol Cell Biol. 2001;21:4909-18 pubmed
	Williams G. Cloning and characterization of two novel thyroid hormone receptor beta isoforms. Mol Cell Biol. 2000;20:8329-42 pubmed
	Davis P, Shih A, Lin H, Martino L, Davis F. Thyroxine promotes association of mitogen-activated protein kinase and nuclear thyroid hormone receptor (TR) and causes serine phosphorylation of TR. J Biol Chem. 2000;275:38032-9 pubmed
	Bhat M, Parkison C, McPhie P, Liang C, Cheng S. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine. Biochem Biophys Res Commun. 1993;195:385-92 pubmed

image

image 1 :

Immunofluorescent analysis of Thyroid Hormone Receptor in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Immunofluorescent analysis of Thyroid Hormone Receptor in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

THRA/THRB Monoclonal Antibody (C3)

Catalog # :

MA1-215

Quantity :

100 µL

Pricing :

US 428

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Human

Clone :

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene.

Immunogen :

Purified fragment of human TR beta-1 corresponding to residues 201-456.

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay-dependent, Immunocytochemistry: 1:20, Immunohistochemistry (Paraffin): 1:20, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

6430529J03Rik; AR7; AW259572; c-erbA-1; C-ERBA-2; c-erbAalpha; c-erbA-alpha; C-ERBA-BETA; CHNG6; EAR7; EAR-7; Erba; ERBA1; ERBA2; ERBA-related 7; ERB-T-1; GRTH; Nr1a1; NR1A2; Nuclear receptor subfamily 1 group A member 1; nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; PRTH; Rvr; T3R[a]; T3Ralpha; THR; THR1; THRA; THRA1; THRA2; THRB; THRB1; THRB2; thyroid hormone nuclear receptor beta variant 1; thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; thyroid hormone receptor beta; thyroid hormone receptor, alpha; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); thyroid normone nuclear receptor alpha variant 1; TR; TR alpha 1; TR alpha 2; triiodothyronine receptor; V-erbA-related protein 7

more info or order :

Invitrogen product webpage