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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Zap-70 Monoclonal Antibody (1E7.2)
catalog :
MA1-21350
quantity :
100 µg
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1E7.2
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
Invitrogen product webpage
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 2
Vroblova V, Smolej L, Krejsek J. Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia. Hematology. 2012;17:268-74 pubmed publisher
  • flow cytometry; mouse
Deswal S, Schulze A, Hofer T, Schamel W. Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks. PLoS ONE. 2011;6:e22928 pubmed publisher
  • flow cytometry; human; fig 2
Degheidy H, Venzon D, Farooqui M, Abbasi F, Arthur D, Wilson W, et al. Methodological comparison of two anti-ZAP-70 antibodies. Cytometry B Clin Cytom. 2011;80:300-8 pubmed publisher
  • flow cytometry; human; tbl 4
Oliveira A, de la Banda E, Domingo Domenech E, Encuentra M, Mercadal S, Domingo A, et al. Prospective study of clinical and biological prognostic factors at diagnosis in patients with early stage B-cell chronic lymphocytic leukemia. Leuk Lymphoma. 2011;52:429-35 pubmed publisher
  • flow cytometry; human; fig 2
Friedrichs B, Siegel S, Reimer R, Barsoum A, Coggin J, Kabelitz D, et al. High expression of the immature laminin receptor protein correlates with mutated IGVH status and predicts a favorable prognosis in chronic lymphocytic leukemia. Leuk Res. 2011;35:721-9 pubmed publisher
  • flow cytometry; human; fig 2
Zucchetto A, Cattarossi I, Nanni P, Zaina E, Prato G, Gilestro M, et al. Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia. Immunol Lett. 2011;134:137-44 pubmed publisher
  • flow cytometry; human; fig 2
Vroblova V, Vrbacky F, Hrudkova M, Jankovicova K, Schmitzova D, Maly J, et al. Significant change in ZAP-70 expression during the course of chronic lymphocytic leukemia. Eur J Haematol. 2010;84:513-7 pubmed publisher
  • immunocytochemistry; human
Zum Büschenfelde C, Wagner M, Lutzny G, Oelsner M, Feuerstacke Y, Decker T, et al. Recruitment of PKC-betaII to lipid rafts mediates apoptosis-resistance in chronic lymphocytic leukemia expressing ZAP-70. Leukemia. 2010;24:141-52 pubmed publisher
  • flow cytometry; human
Friedrichs B, Siegel S, Kloess M, Barsoum A, Coggin J, Rohrer J, et al. Humoral immune responses against the immature laminin receptor protein show prognostic significance in patients with chronic lymphocytic leukemia. J Immunol. 2008;180:6374-84 pubmed
  • flow cytometry; mouse; fig 2
Li F, Ding S, Pan J, Shakhmatov M, Kashentseva E, Wu J, et al. FCRL2 expression predicts IGHV mutation status and clinical progression in chronic lymphocytic leukemia. Blood. 2008;112:179-87 pubmed publisher
  • flow cytometry; human
Gachard N, Salviat A, Boutet C, Arnoulet C, Durrieu F, Lenormand B, et al. Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method. Haematologica. 2008;93:215-23 pubmed publisher
  • flow cytometry; human; fig 5
Saddler C, Ouillette P, Kujawski L, Shangary S, Talpaz M, Kaminski M, et al. Comprehensive biomarker and genomic analysis identifies p53 status as the major determinant of response to MDM2 inhibitors in chronic lymphocytic leukemia. Blood. 2008;111:1584-93 pubmed
  • flow cytometry; human
Preobrazhensky S, Bahler D. Optimization of flow cytometric measurement of ZAP-70 in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2008;74:118-27 pubmed
  • flow cytometry; human; 200 ug/ml; fig 2
Bekkema R, Tadema A, Daenen S, Kluin Nelemans H, Mulder A. An improved flow cytometric method using FACS Lysing Solution for measurement of ZAP-70 expression in B-cell chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2008;74:40-4 pubmed
  • flow cytometry; human
Smolej L, Andrys C, Vroblova V. Modern prognostic factors and angiogenesis in chronic lymphocytic leukemia: more data needed. Leuk Res. 2007;31:1763-4 pubmed
  • flow cytometry; human; tbl 2
Aoun P, Zhou G, Chan W, Page C, Neth K, Pickering D, et al. Familial B-cell chronic lymphocytic leukemia: analysis of cytogenetic abnormalities, immunophenotypic profiles, and immunoglobulin heavy chain gene usage. Am J Clin Pathol. 2007;127:31-8 pubmed
  • flow cytometry; human; fig 3
Zanotti R, Ambrosetti A, Lestani M, Ghia P, Pattaro C, Remo A, et al. ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor. Leukemia. 2007;21:102-9 pubmed
  • flow cytometry; human
Le Garff Tavernier M, Ticchioni M, Brissard M, Salmon C, Raynaud S, Davi F, et al. National standardization of ZAP-70 determination by flow cytometry: the French experience. Cytometry B Clin Cytom. 2007;72:103-8 pubmed
  • flow cytometry; human
Letestu R, Rawstron A, Ghia P, Villamor N, Boeckx N, Leuven N, et al. Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process. Cytometry B Clin Cytom. 2006;70:309-14 pubmed
  • flow cytometry; human
Zucchetto A, Bomben R, Bo M, Nanni P, Bulian P, Rossi F, et al. ZAP-70 expression in B-cell chronic lymphocytic leukemia: evaluation by external (isotypic) or internal (T/NK cells) controls and correlation with IgV(H) mutations. Cytometry B Clin Cytom. 2006;70:284-92 pubmed
  • flow cytometry; human
Rassenti L, Kipps T. Clinical utility of assessing ZAP-70 and CD38 in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:209-13 pubmed
  • flow cytometry; human
Shankey T, Forman M, Scibelli P, Cobb J, Smith C, Mills R, et al. An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:259-69 pubmed
  • flow cytometry; human
Sheridan R, Mounajjed T, Ehrmann D, Hurtubise P, Schrager J. Comparison of bone marrow and peripheral blood ZAP-70 status examined by flow cytometric immunophenotyping in patients with chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:320-1 pubmed
  • flow cytometry; human
Bojarska Junak A, Giannopoulos K, Kowal M, Dmoszynska A, Rolinski J. Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL). Cytometry B Clin Cytom. 2006;70:293-301 pubmed
  • flow cytometry; human
Marti G, Orfao A, Goolsby C. ZAP-70 in CLL: towards standardization of a biomarker for patient management: history of clinical cytometry special issue. Cytometry B Clin Cytom. 2006;70:197-200 pubmed
  • flow cytometry; human
Best O, Ibbotson R, Parker A, Davis Z, Orchard J, Oscier D. ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis. Cytometry B Clin Cytom. 2006;70:235-41 pubmed
  • flow cytometry; human; fig 1
Passam F, Tachynopoulou V, Skoumi D, Tsompanakou A, Stavropoulos Giokas A, Vadikolia C, et al. Feasibility of an easily applicable method of ZAP-70 measurement in chronic lymphocytic leukemia in the routine flow cytometry setting: A methodological approach. Ann Hematol. 2006;85:795-805 pubmed
  • flow cytometry; human; tbl 1
Francis S, Karanth M, Pratt G, Starczynski J, Hooper L, Fegan C, et al. The effect of immunoglobulin VH gene mutation status and other prognostic factors on the incidence of major infections in patients with chronic lymphocytic leukemia. Cancer. 2006;107:1023-33 pubmed
  • flow cytometry; human; fig 1
Scielzo C, Camporeale A, Geuna M, Alessio M, Poggi A, Zocchi M, et al. ZAP-70 is expressed by normal and malignant human B-cell subsets of different maturational stage. Leukemia. 2006;20:689-95 pubmed
  • flow cytometry; mouse; tbl 1
de Totero D, Meazza R, Zupo S, Cutrona G, Matis S, Colombo M, et al. Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells. Blood. 2006;107:3708-15 pubmed
  • flow cytometry; human
Bakke A, Purtzer Z, Leis J, Huang J. A robust ratio metric method for analysis of Zap-70 expression in chronic lymphocytic leukemia (CLL). Cytometry B Clin Cytom. 2006;70:227-34 pubmed
  • flow cytometry; human; tbl 1
Zucchetto A, Bomben R, Dal Bo M, Sonego P, Nanni P, Rupolo M, et al. A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia. J Cell Physiol. 2006;207:354-63 pubmed
  • flow cytometry; human; fig 1
Gibbs G, Bromidge T, Howe D, Hopkins J, Johnson S. Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory. Clin Lab Haematol. 2005;27:258-66 pubmed
González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Zap-70 Monoclonal Antibody (1E7.2)
Catalog # :
MA1-21350
Quantity :
100 µg
Price :
US 425.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.5-1 µg/mL
Species :
Human, Mouse, Rat
Clone :
1E7.2
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Immunogen :
GST-fusion protein of Human ZAP70 of amino acids 282-307 [RRIDTLNSDGYTPEPARITSPDKPRP]
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 0.5-1 µg/mL
Aliases :
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZA70; Zap70; ZAP-70; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA