6x-His Tag Monoclonal Antibody (HIS.H8), Biotin | Invitrogen MA1-21315-BTIN product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Monoclonal Antibody (HIS.H8), Biotin

catalog :

MA1-21315-BTIN

quantity :

50 uL

price :

US 277.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

HIS.H8

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 10

Reference
Han S, Vance J, Jones S, DeCata J, Tran K, Cummings J, et al. Voltage sensor dynamics of a bacterial voltage-gated sodium channel NavAb reveal three conformational states. J Biol Chem. 2023;299:102967 pubmed publisher
Dacon C, Peng L, Lin T, Tucker C, Lee C, Cong Y, et al. Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses. Cell Host Microbe. 2022;: pubmed publisher
Han S, Chu X, Goodson R, Gamel P, Peng S, Vance J, et al. Cholesterol inhibits human voltage-gated proton channel hHv1. Proc Natl Acad Sci U S A. 2022;119:e2205420119 pubmed publisher
Sheikh M, Capicciotti C, Liu L, Praissman J, Ding D, Mead D, et al. Cell surface glycan engineering reveals that matriglycan alone can recapitulate dystroglycan binding and function. Nat Commun. 2022;13:3617 pubmed publisher
Dacon C, Tucker C, Peng L, Lee C, Lin T, Yuan M, et al. Broadly neutralizing antibodies target the coronavirus fusion peptide. bioRxiv. 2022;: pubmed publisher
Wang L, Johnson Z, Wasserman M, Levring J, Chen J, Liu S. Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses. elife. 2020;9: pubmed publisher
Norris S, Jung S, Singh P, Strothman C, Erwin A, Ohi M, et al. Microtubule minus-end aster organization is driven by processive HSET-tubulin clusters. Nat Commun. 2018;9:2659 pubmed publisher
Wendel U, Persson N, Risinger C, Bengtsson E, Nodin B, Danielsson L, et al. A novel monoclonal antibody targeting carboxymethyllysine, an advanced glycation end product in atherosclerosis and pancreatic cancer. PLoS ONE. 2018;13:e0191872 pubmed publisher
Cao Y, Feng Y, Ryser M, Zhu K, Herschlag G, Cao C, et al. Programmable assembly of pressure sensors using pattern-forming bacteria. Nat Biotechnol. 2017;35:1087-1093 pubmed publisher
Vijaya Chandra S, Makhija H, Peter S, Myint Wai C, Li J, Zhu J, et al. Conservative site-specific and single-copy transgenesis in human LINE-1 elements. Nucleic Acids Res. 2016;44:e55 pubmed publisher

product information

Product Type :

Antibody

Product Name :

6x-His Tag Monoclonal Antibody (HIS.H8), Biotin

Catalog # :

MA1-21315-BTIN

Quantity :

50 uL

Price :

US 277.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Tag

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000

Species :

Tag

Clone :

HIS.H8

Isotype :

IgG2b

Storage :

4 C, do not freeze

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6x His synthetic peptide.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 1:500-1:2,000, Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000

Aliases :

6His tag; 6X His; His Tag; Histidine Tag