6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 647 | Invitrogen MA1-21315-A647 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 647

catalog :

MA1-21315-A647

quantity :

50 uL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

AF647

clone name :

HIS.H8

application :

western blot

citations: 3

Reference
Crupi M, Taha Z, Janssen T, Petryk J, Boulton S, Alluqmani N, et al. Oncolytic virus driven T-cell-based combination immunotherapy platform for colorectal cancer. Front Immunol. 2022;13:1029269 pubmed publisher
Joung J, Kirchgatterer P, Singh A, Cho J, Nety S, Larson R, et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun. 2022;13:1606 pubmed publisher
Lei S, Ramesh A, Twitchell E, Wen K, Bui T, Weiss M, et al. High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs. Front Microbiol. 2016;7:1699 pubmed

product information

Product Type :

Antibody

Product Name :

6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 647

Catalog # :

MA1-21315-A647

Quantity :

50 uL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Tag

Applications :

Western Blot: 1:500

Species :

Tag

Clone :

HIS.H8

Isotype :

IgG2b

Storage :

4 C, do not freeze

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6x His synthetic peptide

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:500

Aliases :

6His tag; 6X His; His Tag; Histidine Tag