6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 488 | Invitrogen MA1-21315-A488 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 488

catalog :

MA1-21315-A488

quantity :

50 uL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

HIS.H8

application :

western blot, immunocytochemistry

citations: 6

Reference
Sun H, Liu M, Fan F, Li Z, Fan Y, Zhang J, et al. The Type II Secretory System Mediates Phage Infection in Vibrio cholerae. Front Cell Infect Microbiol. 2021;11:662344 pubmed publisher
Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher
Chi X, Liu X, Wang C, Zhang X, Li X, Hou J, et al. Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain. Nat Commun. 2020;11:4528 pubmed publisher
del Toro D, Carrasquero Ordaz M, Chu A, Ruff T, Shahin M, Jackson V, et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell. 2020;180:323-339.e19 pubmed publisher
Shelke G, Yin Y, Jang S, Lässer C, Wennmalm S, Hoffmann H, et al. Endosomal signalling via exosome surface TGFβ-1. J Extracell Vesicles. 2019;8:1650458 pubmed publisher
Lei S, Ramesh A, Twitchell E, Wen K, Bui T, Weiss M, et al. High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs. Front Microbiol. 2016;7:1699 pubmed

product information

Product Type :

Antibody

Product Name :

6x-His Tag Monoclonal Antibody (HIS.H8), Alexa Fluor 488

Catalog # :

MA1-21315-A488

Quantity :

50 uL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Tag

Applications :

Immunocytochemistry: 1:25-1:100, Western Blot: 1:500

Species :

Tag

Clone :

HIS.H8

Isotype :

IgG2b

Storage :

4 C, do not freeze

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6x His synthetic peptide

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:25-1:100, Western Blot: 1:500

Aliases :

6His tag; 6X His; His Tag; Histidine Tag