TRIM28 Monoclonal Antibody (20C1) | Invitrogen MA1-2023 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TRIM28 Monoclonal Antibody (20C1)

catalog :

MA1-2023

quantity :

100 ug

price :

US 465.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

20C1

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:25	Shibata M, Blauvelt K, Liem K, GARCIA GARCIA M. TRIM28 is required by the mouse KRAB domain protein ZFP568 to control convergent extension and morphogenesis of extra-embryonic tissues. Development. 2011;138:5333-43 pubmed publisher
	Shah P, Boutros Suleiman S, Emanuelli A, Paolini B, Levy Cohen G, Blank M. The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues. Cancers (Basel). 2022;14: pubmed publisher
	Parker K, Gooding A, Valadkhan S, Schiemann W. lncRNA BORG:TRIM28 Complexes Drive Metastatic Progression by Inducing α6 Integrin/CD49f Expression in Breast Cancer Stem Cells. Mol Cancer Res. 2021;19:2068-2080 pubmed publisher
	Palangat M, Anastasakis D, Fei D, Lindblad K, Bradley R, Hourigan C, et al. The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation. Genes Dev. 2019;33:482-497 pubmed publisher
	Emani M, NÃ¤rvÃ¤ E, Stubb A, Chakroborty D, Viitala M, Rokka A, et al. The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency. Stem Cell Reports. 2015;4:519-28 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TRIM28 Monoclonal Antibody (20C1)

Catalog # :

MA1-2023

Quantity :

100 ug

Price :

US 465.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 2 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 ug/mL

Species :

Human, Mouse

Clone :

20C1

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

Immunogen :

Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 2 ug/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-3 ug/mL

Aliases :

AA408787; E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; Krip1; KRIP-1; MommeD9; Nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; tif1 beta; Tif1b; Tif1beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; Trim28; tripartite motif; tripartite motif containing 28; tripartite motif protein 28; tripartite motif-containing 28; tripartite motif-containing protein 28

more info or order :

Invitrogen product webpage