product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2)
catalog :
MA1-2022
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
3F2
reactivity :
human, mouse, bovine
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, other
more info or order :
Invitrogen product webpage
citations: 30
Published Application/Species/Sample/DilutionReference
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; loading ...; fig s1g
Gregory M, D Alessandro A, Alvarez Calderon F, Kim J, Nemkov T, Adane B, et al. ATM/G6PD-driven redox metabolism promotes FLT3 inhibitor resistance in acute myeloid leukemia. Proc Natl Acad Sci U S A. 2016;113:E6669-E6678 pubmed
  • immunocytochemistry; human; 1:2000; fig 6
Marescotti D, Gonzalez Suarez I, Acali S, Johne S, Laurent A, Frentzel S, et al. High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC). J Vis Exp. 2016;: pubmed publisher
  • western blot; human; 1:1000; fig 5
Vijaya Chandra S, Makhija H, Peter S, Myint Wai C, Li J, Zhu J, et al. Conservative site-specific and single-copy transgenesis in human LINE-1 elements. Nucleic Acids Res. 2016;44:e55 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 5
  • western blot; human; 1:1000; fig 5
Hassan M, El Khattouti A, Ejaeidi A, Ma T, Day W, Espinoza I, et al. Elevated Expression of Hepatoma Up-Regulated Protein Inhibits γ-Irradiation-Induced Apoptosis of Prostate Cancer Cells. J Cell Biochem. 2016;117:1308-18 pubmed publisher
  • immunocytochemistry; human; fig s2f
Wang P, Alvarez Perez J, Felsenfeld D, Liu H, Sivendran S, Bender A, et al. A high-throughput chemical screen reveals that harmine-mediated inhibition of DYRK1A increases human pancreatic beta cell replication. Nat Med. 2015;21:383-8 pubmed publisher
  • immunocytochemistry; human
Ferron P, Hogeveen K, Fessard V, Le Hégarat L. Comparative analysis of the cytotoxic effects of okadaic acid-group toxins on human intestinal cell lines. Mar Drugs. 2014;12:4616-34 pubmed publisher
  • western blot; human
Clewell R, Sun B, Adeleye Y, Carmichael P, Efremenko A, McMullen P, et al. Profiling dose-dependent activation of p53-mediated signaling pathways by chemicals with distinct mechanisms of DNA damage. Toxicol Sci. 2014;142:56-73 pubmed publisher
  • immunocytochemistry; human
Jagirdar K, Yin K, Harrison M, Lim W, Muscat G, Sturm R, et al. The NR4A2 nuclear receptor is recruited to novel nuclear foci in response to UV irradiation and participates in nucleotide excision repair. PLoS ONE. 2013;8:e78075 pubmed publisher
  • immunocytochemistry; human; 1:1500
Geiger M, Desanglois G, Hogeveen K, Fessard V, Leprêtre T, Mondeguer F, et al. Cytotoxicity, fractionation and dereplication of extracts of the dinoflagellate Vulcanodinium rugosum, a producer of pinnatoxin G. Mar Drugs. 2013;11:3350-71 pubmed publisher
  • ELISA; bovine
Shimada Y, Goto T, Kawamoto S, Kiso T, Katayama A, Yamanaka Y, et al. Development of a two-step chromatography procedure that allows the purification of a high-purity anti-histone H1 monoclonal immunoglobulin M antibody with immunosuppressant activity. Biomed Chromatogr. 2008;22:13-9 pubmed
Yu C, Sheng Y, Yu F, Ni H, Qiu A, Huang Y, et al. Foxm1 haploinsufficiency drives clonal hematopoiesis and promotes a stress-related transition to hematologic malignancy in mice. J Clin Invest. 2023;133: pubmed publisher
McSwain L, Pillsbury C, Haji Seyed Javadi R, Rath S, Chen V, Huang T, et al. YB1 modulates the DNA damage response in medulloblastoma. Sci Rep. 2023;13:8087 pubmed publisher
Poivre M, Antoine M, Kryshen K, Atsapkina A, Shikov A, Twyffels L, et al. Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: Aristolochia baetica and Magnolia officinalis. Toxins (Basel). 2023;15: pubmed publisher
Raja R, Wu C, Bassoy E, Rubino T, Utagawa E, Magtibay P, et al. PP4 inhibition sensitizes ovarian cancer to NK cell-mediated cytotoxicity via STAT1 activation and inflammatory signaling. J Immunother Cancer. 2022;10: pubmed publisher
Guerrero A, Innes A, Roux P, Buisman S, Jung J, Ortet L, et al. 3-deazaadenosine (3DA) alleviates senescence to promote cellular fitness and cell therapy efficiency in mice. Nat Aging. 2022;2:851-866 pubmed publisher
Vajda F, Szepesi x, V xe1 rady G, Sessler J, Kiss D, Erdei Z, et al. Comparison of Different Clinical Chemotherapeutical Agents' Toxicity and Cell Response on Mesenchymal Stem Cells and Cancer Cells. Cells. 2022;11: pubmed publisher
Marty F, Bettamin L, Thouard A, Bourgade K, Allart S, Larrieu G, et al. Borna disease virus docks on neuronal DNA double-strand breaks to replicate and dampens neuronal activity. iScience. 2022;25:103621 pubmed publisher
Klomp J, Lee Y, Goodwin C, Papke B, Klomp J, Waters A, et al. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell Rep. 2021;37:110060 pubmed publisher
Porosnicu I, Butnaru C, Tiseanu I, Stancu E, Munteanu C, Bita B, et al. Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells. Molecules. 2021;26: pubmed publisher
Yu F, Wei J, Cui X, Yu C, Ni W, Bungert J, et al. Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response. Nucleic Acids Res. 2021;49:5779-5797 pubmed publisher
Boekema B, Stoop M, Vlig M, van Liempt J, Sobota A, Ulrich M, et al. Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing. Appl Microbiol Biotechnol. 2021;105:2057-2070 pubmed publisher
Larose H, Kent T, Ma Q, Shami A, Harerimana N, Li J, et al. Regulation of meiotic progression by Sertoli-cell androgen signaling. Mol Biol Cell. 2020;31:2841-2862 pubmed publisher
Dijksteel G, Ulrich M, Vlig M, Sobota A, Middelkoop E, Boekema B. Safety and bactericidal efficacy of cold atmospheric plasma generated by a flexible surface Dielectric Barrier Discharge device against Pseudomonas aeruginosa in vitro and in vivo. Ann Clin Microbiol Antimicrob. 2020;19:37 pubmed publisher
Salmina K, Bojko A, Inashkina I, Staniak K, Dudkowska M, Podlesniy P, et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci. 2020;21: pubmed publisher
Krajewski A, Gagat M, Zuryn A, Hałas Wiśniewska M, Grzanka D, Grzanka A. Cyclin F is involved in response to cisplatin treatment in melanoma cell lines. Oncol Rep. 2020;43:765-772 pubmed publisher
Ko T, Fujita K, Nomura S, Uemura Y, Yamada S, Tobita T, et al. Quantification of DNA Damage in Heart Tissue as a Novel Prediction Tool for Therapeutic Prognosis of Patients With Dilated Cardiomyopathy. JACC Basic Transl Sci. 2019;4:670-680 pubmed publisher
Hammack C, Ogden S, Madden J, Medina A, Xu C, Phillips E, et al. Zika Virus Infection Induces DNA Damage Response in Human Neural Progenitors That Enhances Viral Replication. J Virol. 2019;93: pubmed publisher
de Moura T, Mozaffari Jovin S, Szabó C, Schmitzová J, Dybkov O, Cretu C, et al. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell. 2018;69:979-992.e6 pubmed publisher
Reid D, Keegan S, Leo Macias A, Watanabe G, Strande N, Chang H, et al. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proc Natl Acad Sci U S A. 2015;112:E2575-84 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2)
Catalog # :
MA1-2022
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: Assay-dependent, Flow Cytometry: 1 ug / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Western Blot: 1 ug/mL
Species :
Human, Mouse
Clone :
3F2
Isotype :
IgG1, kappa
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
Immunogen :
Synthetic peptide sequence surrounding phosphorylated Ser140
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: 1 ug / 10^6 cells, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Western Blot: 1 ug/mL
Aliases :
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA