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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NMDAR2B Monoclonal Antibody (NR2B)
catalog :
MA1-2014
quantity :
100 ug
price :
US 471.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
NR2B
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:1000; loading ...; fig 2h
  • western blot; human; 1:500; loading ...; fig 2c
Chakraborty M, Chen L, Fridel E, Klein M, Senft R, Sarkar A, et al. Overexpression of human NR2B receptor subunit in LMAN causes stuttering and song sequence changes in adult zebra finches. Sci Rep. 2017;7:942 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 7b
Le H, Ahn B, Lee H, Shin A, Chae S, Lee S, et al. Disruption of Ninjurin1 Leads to Repetitive and Anxiety-Like Behaviors in Mice. Mol Neurobiol. 2017;54:7353-7368 pubmed publisher
  • western blot; mouse; fig 3
Lin T, Liu Y, Shih Y, Chen S, Huang T, Chang C, et al. Neurodegeneration in Amygdala Precedes Hippocampus in the APPswe/ PS1dE9 Mouse Model of Alzheimer's Disease. Curr Alzheimer Res. 2015;12:951-63 pubmed
  • western blot; rat; 1:1000
Connors E, Shaik A, Migliore M, Kentner A. Environmental enrichment mitigates the sex-specific effects of gestational inflammation on social engagement and the hypothalamic pituitary adrenal axis-feedback system. Brain Behav Immun. 2014;42:178-90 pubmed publisher
  • western blot; human; 1:1000
Aalbers M, Rijkers K, Majoie H, Dings J, Schijns O, Schipper S, et al. The influence of neuropathology on brain inflammation in human and experimental temporal lobe epilepsy. J Neuroimmunol. 2014;271:36-42 pubmed publisher
  • western blot; mouse; 1:1000
Balosso S, Liu J, Bianchi M, Vezzani A. Disulfide-containing high mobility group box-1 promotes N-methyl-D-aspartate receptor function and excitotoxicity by activating Toll-like receptor 4-dependent signaling in hippocampal neurons. Antioxid Redox Signal. 2014;21:1726-40 pubmed publisher
  • western blot; mouse; 1:1000
Milnerwood A, Kaufman A, Sepers M, Gladding C, Zhang L, Wang L, et al. Mitigation of augmented extrasynaptic NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice. Neurobiol Dis. 2012;48:40-51 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
Fan J, Gladding C, Wang L, Zhang L, Kaufman A, Milnerwood A, et al. P38 MAPK is involved in enhanced NMDA receptor-dependent excitotoxicity in YAC transgenic mouse model of Huntington disease. Neurobiol Dis. 2012;45:999-1009 pubmed publisher
  • immunoprecipitation; mouse
  • western blot; mouse
Fan J, Vasuta O, Zhang L, Wang L, George A, Raymond L. N-methyl-D-aspartate receptor subunit- and neuronal-type dependence of excitotoxic signaling through post-synaptic density 95. J Neurochem. 2010;115:1045-56 pubmed publisher
  • immunocytochemistry; human
Goswami C, Rademacher N, Smalla K, Kalscheuer V, Ropers H, Gundelfinger E, et al. TRPV1 acts as a synaptic protein and regulates vesicle recycling. J Cell Sci. 2010;123:2045-57 pubmed publisher
  • western blot; rat; 1:1000
Montori S, Dos Anjos S, Martínez Villayandre B, Regueiro Purriños M, Gonzalo Orden J, Ruano D, et al. Age and meloxicam attenuate the ischemia/reperfusion-induced down-regulation in the NMDA receptor genes. Neurochem Int. 2010;56:878-85 pubmed publisher
  • immunocytochemistry; rat; 1:800
Pellistri F, Bucciantini M, Relini A, Nosi D, Gliozzi A, Robello M, et al. Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells. J Biol Chem. 2008;283:29950-60 pubmed publisher
Szumlinski K, Herbert J, Mejia Espinoza B, Madory L, Scudder S. Alcohol-drinking during later life by C57BL/6J mice induces sex- and age-dependent changes in hippocampal and prefrontal cortex expression of glutamate receptors and neuropathology markers. Addict Neurosci. 2023;7: pubmed publisher
T xfc rker F, Bharadwaj R, Kleinman J, Weinberger D, Hyde T, White C, et al. Orthogonal approaches required to measure proteasome composition and activity in mammalian brain tissue. J Biol Chem. 2023;299:104811 pubmed publisher
Siddiqui F, Gallagher D, Shuster Hyman H, Lopez L, Gauthier Fisher A, Librach C. First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammation. J Inflamm (Lond). 2023;20:15 pubmed publisher
Filippone A, Cucinotta L, Bova V, Lanza M, Casili G, Paterniti I, et al. Inhibition of LRRK2 Attenuates Depression-Related Symptoms in Mice with Moderate Traumatic Brain Injury. Cells. 2023;12: pubmed publisher
Huerta Sanchez L, Sankaran M, Li T, Doan H, Chiu A, Shulman E, et al. Profiling prefrontal cortex protein expression in rats exhibiting an incubation of cocaine craving following short-access self-administration procedures. Front Psychiatry. 2022;13:1031585 pubmed publisher
Catania M, Colombo L, Sorrentino S, Cagnotto A, Lucchetti J, Barbagallo M, et al. A novel bio-inspired strategy to prevent amyloidogenesis and synaptic damage in Alzheimer's disease. Mol Psychiatry. 2022;27:5227-5234 pubmed publisher
Chen B, Qin G, Xiao J, Deng X, Lin A, Liu H. Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor. J Neuroinflammation. 2022;19:181 pubmed publisher
Niu X, Zheng S, Li S, Liu H. Role of Different Doses of Ketamine in Postoperative Neurocognitive Function in Aged Mice Undergoing Partial Hepatectomy by Regulating the Bmal1/NMDA/NF-Κb Axis. Eur Surg Res. 2022;63:182-195 pubmed publisher
Rodr xed guez Mu xf1 oz M, Cort xe9 s Montero E, Onetti Y, S xe1 nchez Bl xe1 zquez P, Garz xf3 n Ni xf1 o J. The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic Pain. Biomolecules. 2021;11: pubmed publisher
Evans A, Park H, Saw N, Singhal K, Ogawa G, Leib R, et al. Age-related neuroinflammation and pathology in the locus coeruleus and hippocampus: beta-adrenergic antagonists exacerbate impairment of learning and memory in aged mice. Neurobiol Aging. 2021;106:241-256 pubmed publisher
Wang S, He H, Long J, Sui X, Yang J, Lin G, et al. TRPV4 Regulates Soman-Induced Status Epilepticus and Secondary Brain Injury via NMDA Receptor and NLRP3 Inflammasome. Neurosci Bull. 2021;37:905-920 pubmed publisher
Schmidt M, Caron N, Aly A, Lemarié F, Dal Cengio L, Ko Y, et al. DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease. Front Cell Neurosci. 2020;14:590569 pubmed publisher
Jiao L, Zheng M, Duan J, Wu T, Li Z, Liu L, et al. Tau knockout exacerbates degeneration of parvalbumin-positive neurons in substantia nigra pars reticulata in Parkinson's disease-related α-synuclein A53T mice. FASEB J. 2020;34:12239-12254 pubmed publisher
Yennawar M, White R, Jensen F. AMPA Receptor Dysregulation and Therapeutic Interventions in a Mouse Model of CDKL5 Deficiency Disorder. J Neurosci. 2019;39:4814-4828 pubmed publisher
Kang R, Wang L, Sanders S, Zuo K, Hayden M, Raymond L. Altered Regulation of Striatal Neuronal N-Methyl-D-Aspartate Receptor Trafficking by Palmitoylation in Huntington Disease Mouse Model. Front Synaptic Neurosci. 2019;11:3 pubmed publisher
Li Y, Viscidi R, Kannan G, McFarland R, Pletnikov M, Severance E, et al. Chronic Toxoplasma gondii Infection Induces Anti-N-Methyl-d-Aspartate Receptor Autoantibodies and Associated Behavioral Changes and Neuropathology. Infect Immun. 2018;86: pubmed publisher
Kim J, Ko A, Hyun H, Min S, Kim J. PDI regulates seizure activity via NMDA receptor redox in rats. Sci Rep. 2017;7:42491 pubmed publisher
Van Hummel A, Bi M, Ippati S, van der Hoven J, Volkerling A, Lee W, et al. No Overt Deficits in Aged Tau-Deficient C57Bl/6.Mapttm1(EGFP)Kit GFP Knockin Mice. PLoS ONE. 2016;11:e0163236 pubmed publisher
Ampuero E, Jury N, Hartel S, Marzolo M, van Zundert B. Interfering of the Reelin/ApoER2/PSD95 Signaling Axis Reactivates Dendritogenesis of Mature Hippocampal Neurons. J Cell Physiol. 2017;232:1187-1199 pubmed publisher
Takei Y, Kikkawa Y, Atapour N, Hensch T, Hirokawa N. Defects in Synaptic Plasticity, Reduced NMDA-Receptor Transport, and Instability of Postsynaptic Density Proteins in Mice Lacking Microtubule-Associated Protein 1A. J Neurosci. 2015;35:15539-54 pubmed publisher
Li M, Yang S, Xing B, Ferguson B, Gulchina Y, Li Y, et al. LY395756, an mGluR2 agonist and mGluR3 antagonist, enhances NMDA receptor expression and function in the normal adult rat prefrontal cortex, but fails to improve working memory and reverse MK801-induced working memory impairment. Exp Neurol. 2015;273:190-201 pubmed publisher
Farley M, Swulius M, Waxham M. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience. 2015;304:286-301 pubmed publisher
Shin S, Kwon O, Kang J, Kwon S, Oh S, Choi J, et al. mGluR5 in the nucleus accumbens is critical for promoting resilience to chronic stress. Nat Neurosci. 2015;18:1017-24 pubmed publisher
Bustos F, Varela Nallar L, Campos M, Henriquez B, Phillips M, Opazo C, et al. PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors. PLoS ONE. 2014;9:e94037 pubmed publisher
Cone J, Chartoff E, Potter D, Ebner S, Roitman M. Prolonged high fat diet reduces dopamine reuptake without altering DAT gene expression. PLoS ONE. 2013;8:e58251 pubmed publisher
Takahashi N, Sakurai T, Bozdagi Gunal O, Dorr N, Moy J, Krug L, et al. Increased expression of receptor phosphotyrosine phosphatase-?/? is associated with molecular, cellular, behavioral and cognitive schizophrenia phenotypes. Transl Psychiatry. 2011;1:e8 pubmed publisher
Marchand F, D Mello R, Yip P, Calvo M, Muller E, Pezet S, et al. Specific involvement of atypical PKC?/PKM? in spinal persistent nociceptive processing following peripheral inflammation in rat. Mol Pain. 2011;7:86 pubmed publisher
D Mello R, Marchand F, Pezet S, McMahon S, Dickenson A. Perturbing PSD-95 interactions with NR2B-subtype receptors attenuates spinal nociceptive plasticity and neuropathic pain. Mol Ther. 2011;19:1780-92 pubmed publisher
Li Y, Liu G, Hu J, Gao W, Huang Y. Dopamine D(1) receptor-mediated enhancement of NMDA receptor trafficking requires rapid PKC-dependent synaptic insertion in the prefrontal neurons. J Neurochem. 2010;114:62-73 pubmed publisher
Fan J, Cowan C, Zhang L, Hayden M, Raymond L. Interaction of postsynaptic density protein-95 with NMDA receptors influences excitotoxicity in the yeast artificial chromosome mouse model of Huntington's disease. J Neurosci. 2009;29:10928-38 pubmed publisher
Aksenova M, Aksenov M, Adams S, Mactutus C, Booze R. Neuronal survival and resistance to HIV-1 Tat toxicity in the primary culture of rat fetal neurons. Exp Neurol. 2009;215:253-63 pubmed publisher
Lamas M, Lee Rivera I, López Colomé A. Cell-specific expression of N-methyl-D-aspartate receptor subunits in Müller glia and neurons from the chick retina. Invest Ophthalmol Vis Sci. 2005;46:3570-7 pubmed
Osterweil E, Wells D, Mooseker M. A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis. J Cell Biol. 2005;168:329-38 pubmed
El Husseini A, Craven S, Chetkovich D, Firestein B, Schnell E, Aoki C, et al. Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering. J Cell Biol. 2000;148:159-72 pubmed
Wang Y, Bosy T, Yasuda R, Grayson D, Vicini S, Pizzorusso T, et al. Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum. J Neurochem. 1995;65:176-83 pubmed
product information
Product Type :
Antibody
Product Name :
NMDAR2B Monoclonal Antibody (NR2B)
Catalog # :
MA1-2014
Quantity :
100 ug
Price :
US 471.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1-2 ug/mL
Species :
Human, Mouse, Rat
Clone :
NR2B
Isotype :
IgG1
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.
Immunogen :
Recombinant protein: NR2B subunit of amino acid residues 934-1457.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1-2 ug/mL
Aliases :
AW490526; EIEE27; GluN2B; GluRepsilon2; glutamate [NMDA] receptor subunit epsilon-2; glutamate ionotropic receptor NMDA type subunit 2B; glutamate receptor; glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, NMDA2B; glutamate receptor, ionotropic, NMDA2B (epsilon 2); glutamate receptor, ionotropic, N-methyl D-aspartate 2B; GRIN 2B; GRIN2B; HGNC:4586; hNR 3; hNR3; LOW QUALITY PROTEIN: glutamate receptor ionotropic, NMDA 2B; MGC142178; MGC142180; MRD6; Nmdar2b; NMDE2; NME2; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 2B; N-methyl-D-aspartate receptor subunit 3; NR2B; NR3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA