This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
NEFM Monoclonal Antibody (3H11)
catalog :
MA1-2011
quantity :
100 ug
price :
US 502.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3H11

The same clone is also sold as:
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
Reference
Shaw G, Morse S, Ararat M, Graham F. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 2002;16:869-71 pubmed
Benson D, Mandell J, Shaw G, Banker G. Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons. J Neurocytol. 1996;25:181-96 pubmed
product information
Product Type :
Antibody
Product Name :
NEFM Monoclonal Antibody (3H11)
Catalog # :
MA1-2011
Quantity :
100 ug
Price :
US 502.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Rat
Applications :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:500, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
Species :
Human, Rat
Clone :
3H11
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Immunogen :
Synthetic peptide corresponding to a C-terminal fragment of rat neurofilament.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:50-1:500, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:1,000
Aliases :
160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA