NEFL Monoclonal Antibody (DA2) | Invitrogen MA1-2010 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFL Monoclonal Antibody (DA2)

catalog :

MA1-2010

quantity :

100 ug

price :

US 502.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DA2

reactivity :

human, mouse, rat, pigs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; tbl 2	Huizinga R, van der Star B, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31 pubmed publisher
western blot; human	van Noort J, Bsibsi M, Gerritsen W, van der Valk P, Bajramovic J, Steinman L, et al. Alphab-crystallin is a target for adaptive immune responses and a trigger of innate responses in preactive multiple sclerosis lesions. J Neuropathol Exp Neurol. 2010;69:694-703 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; fig 3	Katayama S, Watanabe C, Noda K, Ohishi H, Yamamura Y, Nishisaka T, et al. Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement. J Neurol Sci. 1999;171:72-7 pubmed
	Bu F, Min J, Razzaque M, El Hamamy A, Patrizz A, Qi L, et al. Activation of cerebral Ras-related C3 botulinum toxin substrate (Rac) 1 promotes post-ischemic stroke functional recovery in aged mice. Neural Regen Res. 2024;19:881-886 pubmed publisher
	Giagnorio E, Malacarne C, Cavalcante P, Scandiffio L, Cattaneo M, Pensato V, et al. MiR-146a in ALS: Contribution to Early Peripheral Nerve Degeneration and Relevance as Disease Biomarker. Int J Mol Sci. 2023;24: pubmed publisher
	Bu F, Munshi Y, Furr J, Min J, Qi L, Patrizz A, et al. Activation of neuronal Ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and axonal plasticity in mice. J Neurochem. 2021;157:1366-1376 pubmed publisher
	Ashton N, Leuzy A, Lim Y, Troakes C, Hortobagyi T, Höglund K, et al. Increased plasma neurofilament light chain concentration correlates with severity of post-mortem neurofibrillary tangle pathology and neurodegeneration. Acta Neuropathol Commun. 2019;7:5 pubmed publisher
	Lukiw W, Cong L, Jaber V, Zhao Y. Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture. Front Neurosci. 2018;12:896 pubmed publisher
	Shaw G, Morse S, Ararat M, Graham F. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 2002;16:869-71 pubmed
	Benson D, Mandell J, Shaw G, Banker G. Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons. J Neurocytol. 1996;25:181-96 pubmed
	Edson K, Linck R, Letourneau P. Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system. J Neurosci Res. 1991;30:105-15 pubmed

product information

Product Type :

Antibody

Product Name :

NEFL Monoclonal Antibody (DA2)

Catalog # :

MA1-2010

Quantity :

100 ug

Price :

US 502.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Porcine, Rat

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 2-3 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1-2 ug/mL

Species :

Human, Mouse, Porcine, Rat

Clone :

DA2

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

Immunogen :

Enzymatically dephosphorylated porcine neurofilaments.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 2-3 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 1-2 ug/mL

Aliases :

68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; Nefl; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L

more info or order :

Invitrogen product webpage