LCK Monoclonal Antibody (LCK-01) | Invitrogen MA1-19197 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

LCK Monoclonal Antibody (LCK-01)

catalog :

MA1-19197

quantity :

100 µg

price :

US 399

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Lck-01

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:3000; fig 5	Nguyen T, Carrascal M, Vidal Cortes O, Gallardo O, Casas V, Gay M, et al. The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies. J Proteomics. 2016;131:190-198 pubmed publisher

image

image 1 :

product information

Product Type :

Antibody

Product Name :

LCK Monoclonal Antibody (LCK-01)

Catalog # :

MA1-19197

Quantity :

100 µg

Price :

US 399

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1-5 µg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

LCK-01

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.

Immunogen :

Peptide corresponding to amino acids 22-36 in the sequence of human Lck.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1-5 µg/mL, Immunocytochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

EC 2.7.10.2; Hck-3; IMD22; kinase Lck; Lck; LCK proto-oncogene, Src family tyrosine kinase; Lck1; Lcktkr; leukocyte C-terminal Src kinase; LSK; Lskt; Lsk-t; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56 ; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; tyrosine-protein kinase Lck; YT16

more info or order :

Invitrogen product webpage