beta-3 Tubulin Monoclonal Antibody (TU-20) | Invitrogen MA1-19187 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

beta-3 Tubulin Monoclonal Antibody (TU-20)

catalog :

MA1-19187

quantity :

100 µg

price :

US 491

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TU-20

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:2000; fig 9	Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
immunohistochemistry; human; 1:200	Woehrer A, Laszlo L, Finsterer J, Stollberger C, Furtner J, Rinner W, et al. Novel crystalloid oligodendrogliopathy in hereditary spastic paraplegia. Acta Neuropathol. 2012;124:583-91 pubmed publisher
	CUKIER H, Kim H, Griswold A, Codreanu S, Prince L, Sherrod S, et al. Genomic, transcriptomic, and metabolomic profiles of hiPSC-derived dopamine neurons from clinically discordant brothers with identical PRKN deletions. NPJ Parkinsons Dis. 2022;8:84 pubmed publisher
	Kirchhoff K, Billion A, Voolstra C, Kremb S, Wilke T, Vilcinskas A. Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach. Mar Drugs. 2021;20: pubmed publisher
	Neely M, Davison C, Aschner M, Bowman A. From the Cover: Manganese and Rotenone-Induced Oxidative Stress Signatures Differ in iPSC-Derived Human Dopamine Neurons. Toxicol Sci. 2017;159:366-379 pubmed publisher

image

image 1 :

Immunofluorescent analysis of beta-3 Tubulin (green) in SH-SY5Y cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1?M staurosporine (right panel) for 3 hours and probed with a beta-3 Tubulin monoclonal antibody (Product # MA1-19187), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight at 20X magnification.

product information

Product Type :

Antibody

Product Name :

beta-3 Tubulin Monoclonal Antibody (TU-20)

Catalog # :

MA1-19187

Quantity :

100 µg

Price :

US 491

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Many, Mouse, Rat

Applications :

Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: 1-2 µg/mL, 90 min

Species :

Human, Many, Mouse, Rat

Clone :

TU-20

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Immunogen :

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: 1-2 µg/mL, 90 min

Aliases :

3200002H15Rik; beta 3 tubulin; beta tubulin 3; beta-4; betaIII-tubulin; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; I79_000194; LOC100341581; M(beta)3; M(beta)6; mc1R; melanocortin receptor 1; Neuron specific beta III Tubulin; neuron-specific class III beta-tubulin; TUBB; Tubb3; TUBB4; tubulin beta; tubulin beta 3 class III; Tubulin beta-3; tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; Unknown (protein for MGC:134115)

more info or order :

Invitrogen product webpage