CD45RA Monoclonal Antibody (MEM-56) | Invitrogen MA1-19113 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45RA Monoclonal Antibody (MEM-56)

catalog :

MA1-19113

quantity :

100 µg

price :

US 290.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MEM-56

reactivity :

sooty mangabey, baboons, African green monkey, human, mouse, rhesus macaque

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 48

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2c	Kagoya Y, Nakatsugawa M, Yamashita Y, Ochi T, Guo T, Anczurowski M, et al. BET bromodomain inhibition enhances T cell persistence and function in adoptive immunotherapy models. J Clin Invest. 2016;126:3479-94 pubmed publisher
flow cytometry; human; fig 3d	Ma Q, Garber H, Lu S, He H, Tallis E, Ding X, et al. A novel TCR-like CAR with specificity for PR1/HLA-A2 effectively targets myeloid leukemia in vitro when expressed in human adult peripheral blood and cord blood T cells. Cytotherapy. 2016;18:985-94 pubmed publisher
flow cytometry; human; loading ...; fig s1	Graves S, Kouriba B, Diarra I, Daou M, Niangaly A, Coulibaly D, et al. Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate. Vaccine. 2016;34:2546-55 pubmed publisher
flow cytometry; human	Broos C, van Nimwegen M, Kleinjan A, Ten Berge B, Muskens F, In t Veen J, et al. Impaired survival of regulatory T cells in pulmonary sarcoidosis. Respir Res. 2015;16:108 pubmed publisher
flow cytometry; human	Stenger E, Chiang K, Haight A, Qayed M, Kean L, Horan J. Use of Alefacept for Preconditioning in Multiply Transfused Pediatric Patients with Nonmalignant Diseases. Biol Blood Marrow Transplant. 2015;21:1845-52 pubmed publisher
flow cytometry; human	Grieco A, Billett H, Green N, Driscoll M, Bouhassira E. Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1. PLoS ONE. 2015;10:e0129431 pubmed publisher
flow cytometry; baboons; fig 1	Willis E, Eberle R, Wolf R, White G, McFarlane D. The effects of age and cytomegalovirus on markers of inflammation and lymphocyte populations in captive baboons. PLoS ONE. 2014;9:e107167 pubmed publisher
flow cytometry; human	Kreiser S, Eckhardt J, Kuhnt C, Stein M, Krzyzak L, Seitz C, et al. Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo. Immunobiology. 2015;220:270-9 pubmed publisher
flow cytometry; human	Deng N, Weaver J, Mosmann T. Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFN?- Thpp cells. PLoS ONE. 2014;9:e95986 pubmed publisher
flow cytometry; human; fig 2	van Leeuwen M, Du Pré M, van Wanrooij R, de Ruiter L, Raatgeep H, Lindenbergh Kortleve D, et al. Changes in natural Foxp3(+)Treg but not mucosally-imprinted CD62L(neg)CD38(+)Foxp3(+)Treg in the circulation of celiac disease patients. PLoS ONE. 2013;8:e68432 pubmed publisher
flow cytometry; human	Sharma S, Pichichero M. Functional deficits of pertussis-specific CD4+ T cells in infants compared to adults following DTaP vaccination. Clin Exp Immunol. 2012;169:281-91 pubmed publisher
flow cytometry; human; fig 6	Venigalla R, Guttikonda P, Eckstein V, Ho A, Sertel S, Lorenz H, et al. Identification of a human Th1-like IFN?-secreting Treg subtype deriving from effector T cells. J Autoimmun. 2012;39:377-87 pubmed publisher
flow cytometry; human	Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
flow cytometry; human; fig 1	Imataki O, Ansén S, Tanaka M, Butler M, Berezovskaya A, Milstein M, et al. IL-21 can supplement suboptimal Lck-independent MAPK activation in a STAT-3-dependent manner in human CD8(+) T cells. J Immunol. 2012;188:1609-19 pubmed publisher
flow cytometry; human; fig 8	Neunkirchner A, Leb Reichl V, Schmetterer K, Mutschlechner S, Kueng H, Haiderer D, et al. Human TCR transgenic Bet v 1-specific Th1 cells suppress the effector function of Bet v 1-specific Th2 cells. J Immunol. 2011;187:4077-87 pubmed publisher
flow cytometry; rhesus macaque; fig 3	Belisle S, Yin J, Shedlock D, Dai A, Yan J, Hirao L, et al. Long-term programming of antigen-specific immunity from gene expression signatures in the PBMC of rhesus macaques immunized with an SIV DNA vaccine. PLoS ONE. 2011;6:e19681 pubmed publisher
flow cytometry; human; fig 1, 2	Hoffmann P, Eder R, Edinger M. Polyclonal expansion of human CD4(+)CD25(+) regulatory T cells. Methods Mol Biol. 2011;677:15-30 pubmed publisher
flow cytometry; human; fig 1	Mehling M, Lindberg R, Raulf F, Kuhle J, Hess C, Kappos L, et al. Th17 central memory T cells are reduced by FTY720 in patients with multiple sclerosis. Neurology. 2010;75:403-10 pubmed publisher
flow cytometry; human; fig 2	Bittner S, Bobak N, Herrmann A, Göbel K, Meuth P, Höhn K, et al. Upregulation of K2P5.1 potassium channels in multiple sclerosis. Ann Neurol. 2010;68:58-69 pubmed publisher
flow cytometry; mouse; fig 3	Markley J, Sadelain M. IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice. Blood. 2010;115:3508-19 pubmed publisher
flow cytometry; rhesus macaque; fig 2	Shedlock D, Talbott K, Morrow M, Ferraro B, Hokey D, Muthumani K, et al. Ki-67 staining for determination of rhesus macaque T cell proliferative responses ex vivo. Cytometry A. 2010;77:275-84 pubmed publisher
flow cytometry; human; fig 2	Göbel F, Taschner S, Jurkin J, Konradi S, Vaculik C, Richter S, et al. Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation. Blood. 2009;114:3813-21 pubmed publisher
flow cytometry; human; fig 1	Hoffmann P, Boeld T, Eder R, Huehn J, Floess S, Wieczorek G, et al. Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation. Eur J Immunol. 2009;39:1088-97 pubmed publisher
flow cytometry; human; fig 1e	Park C, Majeti R, Weissman I. In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood. Nat Protoc. 2008;3:1932-40 pubmed publisher
flow cytometry; human; tbl 1	Fujimaki W, Takahashi N, Ohnuma K, Nagatsu M, Kurosawa H, Yoshida S, et al. Comparative study of regulatory T cell function of human CD25CD4 T cells from thymocytes, cord blood, and adult peripheral blood. Clin Dev Immunol. 2008;2008:305859 pubmed publisher
flow cytometry; human; fig 2	Dunham R, Cervasi B, Brenchley J, Albrecht H, Weintrob A, Sumpter B, et al. CD127 and CD25 expression defines CD4+ T cell subsets that are differentially depleted during HIV infection. J Immunol. 2008;180:5582-92 pubmed
flow cytometry; human	Hoves S, Krause S, Schutz C, Halbritter D, Scholmerich J, Herfarth H, et al. Monocyte-derived human macrophages mediate anergy in allogeneic T cells and induce regulatory T cells. J Immunol. 2006;177:2691-8 pubmed
flow cytometry; human	Lim H, Broxmeyer H, Kim C. Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells. J Immunol. 2006;177:840-51 pubmed
flow cytometry; human; fig 1	Jamieson C, Gotlib J, Durocher J, Chao M, Mariappan M, Lay M, et al. The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation. Proc Natl Acad Sci U S A. 2006;103:6224-9 pubmed
flow cytometry; African green monkey flow cytometry; rhesus macaque	Paiardini M, Cervasi B, Sumpter B, McClure H, Sodora D, Magnani M, et al. Perturbations of cell cycle control in T cells contribute to the different outcomes of simian immunodeficiency virus infection in rhesus macaques and sooty mangabeys. J Virol. 2006;80:634-42 pubmed
flow cytometry; human	Schwendemann J, Choi C, Schirrmacher V, Beckhove P. Dynamic differentiation of activated human peripheral blood CD8+ and CD4+ effector memory T cells. J Immunol. 2005;175:1433-9 pubmed
flow cytometry; human	Ku C, Zerboni L, Ito H, Graham B, Wallace M, Arvin A. Varicella-zoster virus transfer to skin by T Cells and modulation of viral replication by epidermal cell interferon-alpha. J Exp Med. 2004;200:917-25 pubmed
flow cytometry; human	Ku C, Padilla J, Grose C, Butcher E, Arvin A. Tropism of varicella-zoster virus for human tonsillar CD4(+) T lymphocytes that express activation, memory, and skin homing markers. J Virol. 2002;76:11425-33 pubmed
flow cytometry; human; fig 1	Jacobsen M, Hoffmann S, Cepok S, Stei S, Ziegler A, Sommer N, et al. A novel mutation in PTPRC interferes with splicing and alters the structure of the human CD45 molecule. Immunogenetics. 2002;54:158-63 pubmed
western blot; human; fig 1	Yu Y, Rabinowitz R, Polliack A, Ben Bassat H, Schlesinger M. Hyposialated 185 kDa CD45RA+ molecules attain a high concentration in B lymphoma cells and in activated human B cells. Eur J Haematol. 2002;68:22-30 pubmed
flow cytometry; human	Blom B, Ho S, Antonenko S, Liu Y. Generation of interferon alpha-producing predendritic cell (Pre-DC)2 from human CD34(+) hematopoietic stem cells. J Exp Med. 2000;192:1785-96 pubmed
flow cytometry; human; fig 2	Sharron M, Pohlmann S, Price K, Lolis E, Tsang M, Kirchhoff F, et al. Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes. Blood. 2000;96:41-9 pubmed
flow cytometry; rhesus macaque flow cytometry; sooty mangabey	Chakrabarti L, Lewin S, Zhang L, Gettie A, Luckay A, Martin L, et al. Normal T-cell turnover in sooty mangabeys harboring active simian immunodeficiency virus infection. J Virol. 2000;74:1209-23 pubmed
flow cytometry; human	Lee B, Sharron M, Montaner L, Weissman D, Doms R. Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages. Proc Natl Acad Sci U S A. 1999;96:5215-20 pubmed
western blot; human	Holada K, Mondoro T, Muller J, Vostal J. Increased expression of phosphatidylinositol-specific phospholipase C resistant prion proteins on the surface of activated platelets. Br J Haematol. 1998;103:276-82 pubmed
flow cytometry; human; fig 4	Kim C, Pelus L, White J, Applebaum E, Johanson K, Broxmeyer H. CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells. J Immunol. 1998;160:2418-24 pubmed
	Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
	Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
	Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
	Böiers C, Richardson S, Laycock E, Zriwil A, Turati V, Brown J, et al. A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1. Dev Cell. 2018;44:362-377.e7 pubmed publisher
	Prezzemolo T, van Meijgaarden K, Franken K, Caccamo N, Dieli F, Ottenhoff T, et al. Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8+ T-cells. Eur J Immunol. 2017;: pubmed publisher
	Nobs S, Natali S, Pohlmeier L, Okreglicka K, Schneider C, Kurrer M, et al. PPARÎ³ in dendritic cells and T cells drives pathogenic type-2 effector responses in lung inflammation. J Exp Med. 2017;214:3015-3035 pubmed publisher
	Riou R, Bressollette Bodin C, Boutoille D, Gagne K, Rodallec A, Lefebvre M, et al. Severe Symptomatic Primary Human Cytomegalovirus Infection despite Effective Innate and Adaptive Immune Responses. J Virol. 2017;91: pubmed publisher

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image 1 :

product information

Product Type :

Antibody

Product Name :

CD45RA Monoclonal Antibody (MEM-56)

Catalog # :

MA1-19113

Quantity :

100 µg

Price :

US 290.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1-4 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

MEM-56

Isotype :

IgG2b

Storage :

4° C, do not freeze

Description :

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

Immunogen :

Human thymocytes and T lymphocytes.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1-4 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

B220; CD45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; LCA; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; Lyt-4; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

more info or order :

Invitrogen product webpage