Cytokeratin 18 Monoclonal Antibody (DC-10) | Invitrogen MA1-19041 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cytokeratin 18 Monoclonal Antibody (DC-10)

catalog :

MA1-19041

quantity :

100 µg

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DC-10

reactivity :

African green monkey, human, mouse, rhesus macaque

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; loading ...; fig 1b immunocytochemistry; human; loading ...; fig 2a western blot; human; loading ...; fig 2e	Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, et al. Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Sci Rep. 2016;6:34472 pubmed publisher
immunocytochemistry; human; fig 3g western blot; human; fig 3h	Kuga T, Sasaki M, Mikami T, Miake Y, Adachi J, Shimizu M, et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Sci Rep. 2016;6:26557 pubmed publisher
western blot; human; fig 5	Thakkar A, Wang B, Picon Ruiz M, Buchwald P, Ince T. Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer. Breast Cancer Res Treat. 2016;157:77-90 pubmed publisher
immunocytochemistry; human; fig s10	Hines W, Yaswen P, Bissell M. Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias. Nat Commun. 2015;6:6927 pubmed publisher
immunohistochemistry; human; 1:100	BaÅŸak K, KiroÄŸlu K. Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report. Medicine (Baltimore). 2014;93:e246 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Zollner G, Thueringer A, Lackner C, Fickert P, Trauner M. Alterations of canalicular ATP-binding cassette transporter expression in drug-induced liver injury. Digestion. 2014;90:81-8 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, et al. A novel mechanism of keratin cytoskeleton organization through casein kinase I? and FAM83H in colorectal cancer. J Cell Sci. 2013;126:4721-31 pubmed publisher
immunoprecipitation; human	Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
immunohistochemistry; human; 1:200	Marian C, Boilă A, Soanca D, Malau M, Podeanu D, Resetkova E, et al. Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature. APMIS. 2013;121:272-9 pubmed publisher
immunohistochemistry; human; 1:500	Szponar A, Kovacs G. Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas. Virchows Arch. 2012;460:423-7 pubmed publisher
western blot; mouse	Singla A, Moons D, Snider N, Wagenmaker E, Jayasundera V, Omary M. Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria. Hepatology. 2012;56:322-31 pubmed publisher
western blot; mouse; 1:1000	Ku N, Toivola D, Strnad P, Omary M. Cytoskeletal keratin glycosylation protects epithelial tissue from injury. Nat Cell Biol. 2010;12:876-85 pubmed publisher
immunohistochemistry; human; 1:500	Robertson F, Ogasawara M, Ye Z, Chu K, Pickei R, Debeb B, et al. Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype. J Biomol Screen. 2010;15:820-9 pubmed publisher
western blot; human; 1:2000	Yalcin Ozuysal O, Fiche M, Guitierrez M, Wagner K, Raffoul W, Brisken C. Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates. Cell Death Differ. 2010;17:1600-12 pubmed publisher
immunohistochemistry; African green monkey	Wood C, Kaplan J, Fontenot M, Williams J, Cline J. Endometrial profile of tamoxifen and low-dose estradiol combination therapy. Clin Cancer Res. 2010;16:946-56 pubmed publisher
flow cytometry; human	Metallo C, Ji L, de Pablo J, Palecek S. Directed differentiation of human embryonic stem cells to epidermal progenitors. Methods Mol Biol. 2010;585:83-92 pubmed publisher
western blot; human; 1:200	Chang C, Chiou P, Chen W, Li Y, Yiu J, Cheng Y, et al. Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines. Res Vet Sci. 2010;88:285-93 pubmed publisher
immunohistochemistry; human	Yu G, Goodloe S, D Angelis C, McGrath B, Chen F. Giant clear cell hidradenoma of the knee. J Cutan Pathol. 2010;37:e37-41 pubmed publisher
immunohistochemistry; mouse; 1:100	Røsland G, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009;69:5331-9 pubmed publisher
immunocytochemistry; human	Sumitran Holgersson S, Nowak G, Thowfeequ S, Begum S, Joshi M, Jaksch M, et al. Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas. Cell Transplant. 2009;18:183-93 pubmed
immunohistochemistry; human; 1:100	Li H, Zhou G, Fu X, Zhang L. Antigen expression of human eccrine sweat glands. J Cutan Pathol. 2009;36:318-24 pubmed publisher
western blot; human	Guo Y, Xie J, Rubin E, Tang Y, Lin F, Zi X, et al. Frzb, a secreted Wnt antagonist, decreases growth and invasiveness of fibrosarcoma cells associated with inhibition of Met signaling. Cancer Res. 2008;68:3350-60 pubmed publisher
ELISA; human; 10 ug/ml	Tao G, Li D, Zhou Q, Toivola D, Strnad P, Sandesara N, et al. Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation. J Pathol. 2008;215:164-74 pubmed publisher
immunohistochemistry; rhesus macaque; 1:100	Wood C, Hester J, Cline J. Mammary gland development in early pubertal female macaques. Toxicol Pathol. 2007;35:795-805 pubmed
western blot; human	Tao G, Strnad P, Zhou Q, Kamal A, Zhang L, Madani N, et al. Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease. Clin Gastroenterol Hepatol. 2007;5:857-64 pubmed
immunohistochemistry; human	Gurbuz Y, Kose N, Aydin O, Ozturk M. An immunohistochemical profile of giant cell carcinoma of the larynx. Auris Nasus Larynx. 2007;34:413-6 pubmed
immunohistochemistry; human; 1:500	Kalantari F, Miao D, Emadali A, Tzimas G, Goltzman D, Vali H, et al. Cellular and molecular mechanisms of abnormal calcification following ischemia-reperfusion injury in human liver transplantation. Mod Pathol. 2007;20:357-66 pubmed
western blot; human	Guo Y, Zi X, Koontz Z, Kim A, Xie J, Gorlick R, et al. Blocking Wnt/LRP5 signaling by a soluble receptor modulates the epithelial to mesenchymal transition and suppresses met and metalloproteinases in osteosarcoma Saos-2 cells. J Orthop Res. 2007;25:964-71 pubmed
western blot; human	Tao G, Toivola D, Zhou Q, Strnad P, Xu B, Michie S, et al. Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner. J Cell Sci. 2006;119:1425-32 pubmed
western blot; human	Liu Y, Liu H, Han B, Zhang J. Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis. Cancer Res. 2006;66:3248-55 pubmed
immunohistochemistry; human	Tao G, Nakamichi I, Ku N, Wang J, Frolkis M, Gong X, et al. Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res. 2006;312:411-22 pubmed
western blot; human	Zi X, Guo Y, Simoneau A, Hope C, Xie J, Holcombe R, et al. Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness. Cancer Res. 2005;65:9762-70 pubmed
	Peng B, Qiu X, Dong Z, Zhang J, Pei Y, Wang T. Proteomic profiling of biomarkers by MALDI-TOF mass spectrometry for the diagnosis of tracheobronchial stenosis after tracheobronchial tuberculosis. Exp Ther Med. 2021;21:63 pubmed publisher
	Németh I, Tiszlavicz L. Biphenotypic surface epithelial cells in the gastrointestinal tube with mixed epithelial-myofibroblastic differentiation: a paradigm. Pathol Oncol Res. 2012;18:391-6 pubmed publisher
	Ku N, Toivola D, Zhou Q, Tao G, Zhong B, Omary M. Studying simple epithelial keratins in cells and tissues. Methods Cell Biol. 2004;78:489-517 pubmed
	Toivola D, Ku N, Resurreccion E, Nelson D, Wright T, Omary M. Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease. Hepatology. 2004;40:459-66 pubmed
	Apaydin R, Gurbuz Y, Bayramgurler D, Muezzinoglu B, Bilen N. Cytokeratin expression in lichen amyloidosus and macular amyloidosis. J Eur Acad Dermatol Venereol. 2004;18:305-9 pubmed
	Ku N, Soetikno R, Omary M. Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury. Hepatology. 2003;37:1006-14 pubmed
	Ku N, Gish R, Wright T, Omary M. Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med. 2001;344:1580-7 pubmed

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image 1 :

product information

Product Type :

Antibody

Product Name :

Cytokeratin 18 Monoclonal Antibody (DC-10)

Catalog # :

MA1-19041

Quantity :

100 µg

Price :

US 496.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL

Species :

Human

Clone :

DC-10

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Immunogen :

Human breast carcinoma cell line PMC-42.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Flow Cytometry: 1-4 µg/mL, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay-dependent, Western Blot: 1-2 µg/mL

Aliases :

cb83; Cell proliferation-inducing gene 46 protein; CK 18; CK18; CK-18; CYK18; cytokeratin 18; cytokeratin endo B; cytokeratin type -1; DAPK-1; Cytokeratin18; cytokeratin-18; dapk1; dorsal aorta proneprin kinesin-1; DreK18; Endo B; I79_023185; K 18; K18; keratin 18; keratin 18, type I; keratin 18a, tandem duplicate 1; keratin complex 1, acidic, gene 18; keratin D; Keratin, type I cytoskeletal 18; Keratin18; keratin-18; Kerd; KRT 18; Krt1-18; KRT18; krt18 {ECO:0000250; krt18a.1; LOC100008885; PIG46; sb:cb83; type I cytoskeletal 18; UniProtKB:P05783}; wu:fa13f02; wu:fb36b04

more info or order :

Invitrogen product webpage