Blimp-1 Monoclonal Antibody (3H2-E8) | Invitrogen MA1-16874 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Blimp-1 Monoclonal Antibody (3H2-E8)

catalog :

MA1-16874

quantity :

100 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H2-E8

reactivity :

human, mouse, pigs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; pigs ; fig 2	Zhang Y, Ma J, Li H, Lv J, Wei R, Cong Y, et al. bFGF signaling-mediated reprogramming of porcine primordial germ cells. Cell Tissue Res. 2016;364:429-41 pubmed publisher
immunohistochemistry; mouse; 1:20	Weber S, Eckert D, Nettersheim D, Gillis A, Schäfer S, Kuckenberg P, et al. Critical function of AP-2 gamma/TCFAP2C in mouse embryonic germ cell maintenance. Biol Reprod. 2010;82:214-23 pubmed publisher

image

image 1 :

IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice.

image 2 :

Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10µm). Positive surface epithelium and centrocytes are labelled.

image 3 :

Western Blot detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using MA1-16874. 1881: murine pre-B cell lysate (negative control).

product information

Product Type :

Antibody

Product Name :

Blimp-1 Monoclonal Antibody (3H2-E8)

Catalog # :

MA1-16874

Quantity :

100 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Porcine

Applications :

ChIP Assay: 1 µg/10 µg of protein, ELISA: 1:100-1:2,000, Flow Cytometry: 8 µg/mL, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000

Species :

Human, Mouse, Porcine

Clone :

3H2-E8

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Immunogen :

A fragment of the mouse Blimp-1 (residues 199-409).

Format :

Liquid

Applications w/Dilutions :

Aliases :

B lymphocyte-induced maturation protein 1; b2b1765Clo; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; Blimp1; BLIMP-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PR/SET domain 1; PRDI BF1; PRDI-BF1; PRDI-binding factor-1; Prdm1; RP1-134E15.1; ZNFPR1A1

more info or order :

Invitrogen product webpage