Caspase 9 Monoclonal Antibody (LAP6 96-2-22) | Invitrogen MA1-16842 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Caspase 9 Monoclonal Antibody (LAP6 96-2-22)

catalog :

MA1-16842

quantity :

200 µL

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LAP6 96-2-22

reactivity :

human, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; domestic sheep; 1:10	Gulbahar M, Kabak Y, Karayigit M, Yarim M, Guvenc T, Parlak U. The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs. J Vet Sci. 2011;12:65-73 pubmed
immunohistochemistry; human; 1:200	Vital Reyes V, Rodríguez Burford C, Chhieng D, Alvarado Cabrero I, Reyes Fuentes A, Grizzle W. Ovarian expression of markers associated with proliferation or apoptosis in women with diminished ovarian reserve. Fertil Steril. 2006;86:176-85 pubmed
	Senichkin V, Prokhorova E, Zhivotovsky B, Kopeina G. Simple and Efficient Protocol for Subcellular Fractionation of Normal and Apoptotic Cells. Cells. 2021;10: pubmed publisher

image

image 1 :

The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.

product information

Product Type :

Antibody

Product Name :

Caspase 9 Monoclonal Antibody (LAP6 96-2-22)

Catalog # :

MA1-16842

Quantity :

200 µL

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry (Paraffin): 8-25 µg/mL, Western Blot: 1 µg/mL

Species :

Human

Clone :

LAP6 96-2-22

Isotype :

IgG1, kappa

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

Immunogen :

Recombinant human Caspase 9 pro-domain protein.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2-10 µg/mL, Immunohistochemistry (Paraffin): 8-25 µg/mL, Western Blot: 1 µg/mL

Aliases :

25 kDa caspase-9 dominant negative protein; AI115399; APAF3; APAF-3; apoptotic protease activating factor 3; apoptotic protease MCH-6; Apoptotic protease-activating factor 3; AW493809; cas9; CASP 9; CASP9; CASP-9; Casp9_v1; Casp-9-CTD; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; Caspase9; caspase-9; caspase-9 dominant negative form; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; caspase-9-carboxyl-terminal divergent; CTD; Ice-Lap6; ICE-like apoptotic protease 6; Mch6; OTTHUMP00000002324; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3

more info or order :

Invitrogen product webpage