product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GAPDH Monoclonal Antibody (1D4)
catalog :
MA1-16757
quantity :
100 µL
price :
US 460.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D4
reactivity :
Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , zebrafish , cat, domestic sheep, domestic rabbit, domestic horse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 40
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:40,000; loading ...
Yu X, Curlik D, Oh M, Yin J, Disterhoft J. CREB overexpression in dorsal CA1 ameliorates long-term memory deficits in aged rats. elife. 2017;6: pubmed publisher
  • western blot; mouse; loading ...; fig 4
Yang T, Wang J, Pang Y, Dang X, Ren H, Liu Y, et al. Emodin suppresses silica-induced lung fibrosis by promoting Sirt1 signaling via direct contact. Mol Med Rep. 2016;14:4643-4649 pubmed publisher
  • western blot; human
Bulk E, Ay A, Hammadi M, Ouadid Ahidouch H, Schelhaas S, Hascher A, et al. Epigenetic dysregulation of KCa 3.1 channels induces poor prognosis in lung cancer. Int J Cancer. 2015;137:1306-17 pubmed publisher
  • western blot; human
Xue Y, Zhang H, Wang H, Hu J, Du M, Zhu M. Host inflammatory response inhibits Escherichia coli O157:H7 adhesion to gut epithelium through augmentation of mucin expression. Infect Immun. 2014;82:1921-30 pubmed publisher
  • western blot; human; 1:5000
Jandu S, Webb A, Pak A, Sevinc B, Nyhan D, Belkin A, et al. Nitric oxide regulates tissue transglutaminase localization and function in the vasculature. Amino Acids. 2013;44:261-9 pubmed publisher
Yang M, Luo J, Zhang S, Huang Q, Cao Q. Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway. Open Med (Wars). 2023;18:20230687 pubmed publisher
Li Y, Zhang C, Zhao Z. CircSLCO3A1 depletion ameliorates lipopolysaccharide-induced inflammation and apoptosis of human pulmonary alveolar epithelial cells through the miR-424-5p/HMGB3 pathway. Mol Cell Toxicol. 2023;:1-12 pubmed publisher
He L, Shi Y. Reduced glutathione ameliorates acute kidney injury by inhibiting ferroptosis. Mol Med Rep. 2023;27: pubmed publisher
Mao Z, Nakamura F. Interaction of LARP4 to filamin A mechanosensing domain regulates cell migrations. Front Cell Dev Biol. 2023;11:1152109 pubmed publisher
Sui C, Zhou D. ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway. Exp Ther Med. 2023;25:260 pubmed publisher
Fu X, Mishra R, Chen L, Arfat M, Sharma S, Kingsbury T, et al. Exosomes mediated fibrogenesis in dilated cardiomyopathy through a MicroRNA pathway. iScience. 2023;26:105963 pubmed publisher
J xe4 nsch M, Lubomirov L, Trum M, Williams T, Schmitt J, Schuh K, et al. Inducible over-expression of cardiac Nos1ap causes short QT syndrome in transgenic mice. FEBS Open Bio. 2023;13:118-132 pubmed publisher
Peng S, Luo Y, Chen L, Dai K, Wang Q. lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB. Open Med (Wars). 2022;17:1999-2012 pubmed publisher
Li F, Negi V, Yang P, Lee J, Ma K, Moulik M, et al. TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism. Nucleic Acids Res. 2022;50:12723-12738 pubmed publisher
Zhang H, Du Y, Xin P, Man X. The LINC00852/miR-29a-3p/JARID2 axis regulates the proliferation and invasion of prostate cancer cell. BMC Cancer. 2022;22:1269 pubmed publisher
Blanco L, Patiño Martínez E, Nakabo S, Zhang M, Pedersen H, Wang X, et al. Modulation of the Itaconate Pathway Attenuates Murine Lupus. Arthritis Rheumatol. 2022;74:1971-1983 pubmed publisher
Taiarol L, Bigogno C, Sesana S, Kravicz M, Viale F, Pozzi E, et al. Givinostat-Liposomes: Anti-Tumor Effect on 2D and 3D Glioblastoma Models and Pharmacokinetics. Cancers (Basel). 2022;14: pubmed publisher
Feng G, Sun H, Piao M. FBXL6 is dysregulated in keloids and promotes keloid fibroblast growth by inducing c-Myc expression. Int Wound J. 2022;: pubmed publisher
Owumi S, Kazeem A, Wu B, Ishokare L, Arunsi U, Oyelere A. Apigeninidin-rich Sorghum bicolor (L. Moench) extracts suppress A549 cells proliferation and ameliorate toxicity of aflatoxin B1-mediated liver and kidney derangement in rats. Sci Rep. 2022;12:7438 pubmed publisher
Nguyen M, McAvoy K, Liao S, Doric Z, Lo I, Li H, et al. Mouse midbrain dopaminergic neurons survive loss of the PD-associated mitochondrial protein CHCHD2. Hum Mol Genet. 2022;31:1500-1518 pubmed publisher
Zhang M, Yu G, Liu G, Liu W. Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR-433-3p/ IGF1R axis. J Cell Mol Med. 2022;26:1806-1816 pubmed publisher
Deng P, Halmai J, Beitnere U, Cameron D, Martinez M, Lee C, et al. An in vivo Cell-Based Delivery Platform for Zinc Finger Artificial Transcription Factors in Pre-clinical Animal Models. Front Mol Neurosci. 2021;14:789913 pubmed publisher
Qu X, Sun Z, Wang Y, Ong H. Zoledronic acid promotes osteoclasts ferroptosis by inhibiting FBXO9-mediated p53 ubiquitination and degradation. Peerj. 2021;9:e12510 pubmed publisher
Marullo R, Castro M, Yomtoubian S, Calvo Vidal M, Revuelta M, Krumsiek J, et al. The metabolic adaptation evoked by arginine enhances the effect of radiation in brain metastases. Sci Adv. 2021;7:eabg1964 pubmed publisher
Wang J, Shen J. LncRNA HOXA11-AS aggravates the keloid formation by targeting miR-148b-3p/IGFBP5 axis. Biochem Biophys Res Commun. 2021;581:60-67 pubmed publisher
Azeem W, Bakke R, Gabriel B, Appel S, Øyan A, Kalland K. Evaluation of β-Catenin Inhibition of Axitinib and Nitazoxanide in Human Monocyte-Derived Dendritic Cells. Biomedicines. 2021;9: pubmed publisher
Hu X, Lou T, Yuan C, Wang Y, Tu X, Wang Y, et al. Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells. Oncol Lett. 2021;22:621 pubmed publisher
Chen C, Kawasumi M, Lan T, Poon C, Lin Y, Wu P, et al. Adaptation to Endoplasmic Reticulum Stress Enhances Resistance of Oral Cancer Cells to Cisplatin by Up-Regulating Polymerase η and Increasing DNA Repair Efficiency. Int J Mol Sci. 2020;22: pubmed publisher
Yu C, Liu C, Lin T, Su N, Yang L, Chang Y. The regulation of Oct4 in human gingival fibroblasts stimulated by cyclosporine A: Preliminary observations. J Dent Sci. 2020;15:176-180 pubmed publisher
Li B, Zhao H, Wu Y, Zhu Y, Zhang J, Yang G, et al. Mitochondrial-Derived Vesicles Protect Cardiomyocytes Against Hypoxic Damage. Front Cell Dev Biol. 2020;8:214 pubmed publisher
Hung K, Liao P, Chen C, Chiu Y, Cheng D, Kawasumi M, et al. ASB6 Promotes the Stemness Properties and Sustains Metastatic Potential of Oral Squamous Cell Carcinoma Cells by Attenuating ER Stress. Int J Biol Sci. 2019;15:1080-1090 pubmed publisher
Cargnin F, Kwon J, Katzman S, Chen B, Lee J, Lee S. FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections. Neuron. 2018;100:1083-1096.e5 pubmed publisher
Corona C, Pasini S, Liu J, Amar F, Greene L, Shelanski M. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci. 2018;38:6102-6113 pubmed publisher
Chittoor Vinod V, Lee S, Judge S, Notterpek L. Inducible HSP70 is critical in preventing the aggregation and enhancing the processing of PMP22. ASN Neuro. 2015;7: pubmed publisher
Chen C, Woodruff M, Chen F, Chen Y, Cilluffo M, Tranchina D, et al. Modulation of mouse rod response decay by rhodopsin kinase and recoverin. J Neurosci. 2012;32:15998-6006 pubmed publisher
Moritz O, Tam B, Papermaster D, Nakayama T. A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern. J Biol Chem. 2001;276:28242-51 pubmed
Cideciyan A, Hood D, Huang Y, Banin E, Li Z, Stone E, et al. Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man. Proc Natl Acad Sci U S A. 1998;95:7103-8 pubmed
DeCaluwé G, Degrip W. Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin. Biochem J. 1996;320 ( Pt 3):807-15 pubmed
Molday R, Mackenzie D. Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes. Biochemistry. 1983;22:653-60 pubmed
Hodges R, Heaton R, Parker J, Molday L, Molday R. Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin. J Biol Chem. 1988;263:11768-75 pubmed
image
image 1 :
Invitrogen MA1-16757 image 1
Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (MA1-16757) [green] and an anti-NF-H [red], counterstained with a fluorescent DNA probe [blue].
image 2 :
Invitrogen MA1-16757 image 2
Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots. Blots were probed with MA1-16757 mouse monoclonal antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Antibody was used at dilution of 1:1,000. Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38kDa, the expected molecular weight for GAPDH.
product information
Product Type :
Antibody
Product Name :
GAPDH Monoclonal Antibody (1D4)
Catalog # :
MA1-16757
Quantity :
100 µL
Price :
US 460.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Avian, Bovine, Canine, Chicken, Drosophila, Equine, Feline, Hamster, Human, Marsupial, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Zebrafish
Applications :
ChIP Assay: 1:10 -1:500, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Species :
Avian, Bovine, Canine, Chicken, Drosophila, Equine, Feline, Hamster, Human, Marsupial, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Zebrafish
Clone :
1D4
Isotype :
IgM
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
Immunogen :
Extensively purified pig GAPDH.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 1:10 -1:500, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Aliases :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; GAPD; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; Gapds; Gapd-s; glceraldehyde-3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (G3PDH); glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; multifunctional protein, glycolytic enzyme; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); unnamed protein product; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA