NSE Monoclonal Antibody (5E2) | Invitrogen MA1-16696 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NSE Monoclonal Antibody (5E2)

catalog :

MA1-16696

quantity :

100 µg

price :

425 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5E2

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:8000; loading ...; fig 1e	Czirr E, Castello N, Mosher K, Castellano J, Hinkson I, Lucin K, et al. Microglial complement receptor 3 regulates brain A? levels through secreted proteolytic activity. J Exp Med. 2017;214:1081-1092 pubmed publisher
western blot; mouse; 1:1000; fig 7	O Brien C, Bonanno L, Zhang H, Wyss Coray T. Beclin 1 regulates neuronal transforming growth factor-Î² signaling by mediating recycling of the type I receptor ALK5. Mol Neurodegener. 2015;10:69 pubmed publisher
	He Y, Zhang H, Yung A, Villeda S, Jaeger P, Olayiwola O, et al. ALK5-dependent TGF-? signaling is a major determinant of late-stage adult neurogenesis. Nat Neurosci. 2014;17:943-52 pubmed publisher

product information

Product Type :

Antibody

Product Name :

NSE Monoclonal Antibody (5E2)

Catalog # :

MA1-16696

Quantity :

100 µg

Price :

425 USD

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:100-1:2000, Immunohistochemistry (Paraffin): 1:100-1:300, Western Blot: 1:100-1:2000

Species :

Human

Clone :

5E2

Isotype :

IgG2a

Storage :

4° C, do not freeze

Description :

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Immunogen :

Purified full length native human NSE protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:100-1:2000, Immunohistochemistry (Paraffin): 1:100-1:300, Western Blot: 1:100-1:2000

Aliases :

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375e; Eno; Eno2; Eno-2; ENOG; enolase 2; enolase 2 (gamma, neuronal); enolase 2, gamma neuronal; enolase 2, gamma, neuronal; epididymis secretory protein Li 279; gamma enolase; gamma-enolase; HEL-S-279; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE; RNEN3