CUGBP1 Monoclonal Antibody (3B1) | Invitrogen MA1-16675 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CUGBP1 Monoclonal Antibody (3B1)

catalog :

MA1-16675

quantity :

100 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B1

reactivity :

human, mouse, rat, bovine, pigs , domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:500 western blot; human; 1:500	Santoro M, Modoni A, Masciullo M, Gidaro T, Broccolini A, Ricci E, et al. Analysis of MTMR1 expression and correlation with muscle pathological features in juvenile/adult onset myotonic dystrophy type 1 (DM1) and in myotonic dystrophy type 2 (DM2). Exp Mol Pathol. 2010;89:158-68 pubmed publisher

image

image 1 :

Detection of CUG-BP1 in several cell lysates using MA1-16675.

image 2 :

Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts using MA1-16675.

product information

Product Type :

Antibody

Product Name :

CUGBP1 Monoclonal Antibody (3B1)

Catalog # :

MA1-16675

Quantity :

100 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat

Applications :

Flow Cytometry: 1 µg per million cells, Gel Shift: Assay-Dependent, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: Assay-Dependent, Western Blot: 1:200-1:500

Species :

Bovine, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat

Clone :

3B1

Isotype :

IgG1, kappa

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG) n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG) 8 oligonucleotides in vitro. CUG-BP1 is the major (CUG) 8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3-UTR. The (CUG) n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts.

Immunogen :

CUG-BP1 human nuclear RNA binding protein.

Format :

Liquid

Applications w/Dilutions :

Aliases :

1600010O03Rik; 50 kDa nuclear polyadenylated RNA-binding protein; AA407467; Brain protein F41; Brunol2; bruno-like 2; bruno-like protein 2; Celf1; CELF-1; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; Cugbp; CUG-BP; CUG-BP- and ETR-3-like factor 1; CUGBP Ela; CUGBP Elav-like family member 1; CUGBP, Elav-like family member 1; CUGBP, Elav-like family member 1-like; CUGBP1; CUG-BP1; D2Wsu101e; Deadenylation factor CUG-BP; deadenylation factor EDEN-BP; EDEN-BP; EDEN-BP homolog; embryo deadenylation element binding protein; embryo deadenylation element-binding protein homolog; HNAB50; NAB50; NAPOR; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2; RP23-147D3.7

more info or order :

Invitrogen product webpage