p19ARF Monoclonal Antibody (12-A1-1) | Invitrogen MA1-16664 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p19ARF Monoclonal Antibody (12-A1-1)

catalog :

MA1-16664

quantity :

100 µL

price :

US 436.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

12-A1-1

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; fig 1 western blot; mouse; fig 1	Hobbs R, Batazzi A, Han M, Coulombe P. Loss of Keratin 17 induces tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo. Oncogene. 2016;35:5653-5662 pubmed publisher
western blot; mouse	Funaki T, Kon S, Ronn R, Henmi Y, Kobayashi Y, Watanabe T, et al. Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport. Cell Struct Funct. 2011;36:83-95 pubmed

image

image 1 :

Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with MA1-16664, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).

image 2 :

image 3 :

Western blot analysis of endogenous p19Arf using MA1-16664. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf MA1-16664.

product information

Product Type :

Antibody

Product Name :

p19ARF Monoclonal Antibody (12-A1-1)

Catalog # :

MA1-16664

Quantity :

100 µL

Price :

US 436.00

Clonality :

Monoclonal

Host :

Rat

Reactivity :

Mouse, Rat

Applications :

ELISA: 1:5,000-1:10,000, Immunocytochemistry: 1:10-1:500, Immunohistochemistry (Frozen): 1:10-1:500, Immunohistochemistry (Paraffin): 1:10-1:500, Immunoprecipitation: 1:100, Western Blot: 1:500

Species :

Mouse, Rat

Clone :

12-A1-1

Isotype :

IgG2b

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

Immunogen :

Synthetic peptide containing amino acids within 1-100 of the murine p19ARF.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:5,000-1:10,000, Immunocytochemistry: 1:10-1:500, Immunohistochemistry (Frozen): 1:10-1:500, Immunohistochemistry (Paraffin): 1:10-1:500, Immunoprecipitation: 1:100, Western Blot: 1:500

Aliases :

Alternative reading frame; Arf; ARF-INK4a; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; CDKN2A; cdkn2a {ECO:0000312; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; CMM2; cyclin dependent kinase inhibitor 2A; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3-like; cyclin-dependent kinase inhibitor protein; EMBL:AAL76336.1, ECO:0000312; EMBL:AAL76338.1, ECO:0000312; INK4; INK4A; Ink4a/Arf; INK4a-ARF; LOC100764983; mitochondrial smARF; MLM; MTS1; MTS-1; Multiple tumor suppressor 1; P14; P14ARF; p16; p16(INK4a); p16Cdkn2a; P16INK4; p16-INK4; P16INK4A; p16-INK4a; P19; p19 ; p19ARF; Pctr1; RGD:2323}; TP16; Tumor suppressor ARF

more info or order :

Invitrogen product webpage