RPE65 Monoclonal Antibody (401.8B11.3D9) | Invitrogen MA1-16578 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RPE65 Monoclonal Antibody (401.8B11.3D9)

catalog :

MA1-16578

quantity :

200 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

401.8B11.3D9

reactivity :

human, mouse, rat, dogs, chicken, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100	Mathivanan I, Trepp C, Brunold C, Baerlocher G, Enzmann V. Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro. Exp Cell Res. 2015;333:11-20 pubmed publisher
western blot; human western blot; mouse	Lee H, Chung H, Arnouk H, Lamoke F, Hunt R, Hrushesky W, et al. Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress. Int J Biol Macromol. 2010;47:104-8 pubmed publisher
	Dong C, Zou D, Duan H, Hu X, Zhou Q, Shi W, et al. Ex vivo cultivated retinal pigment epithelial cell transplantation for the treatment of rabbit corneal endothelial dysfunction. Eye Vis (Lond). 2023;10:34 pubmed publisher
	Wei Y, Alexandre U, Ma X. Hydrogels to Support Transplantation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells. Brain Sci. 2022;12: pubmed publisher
	Wu L, Yang S, Li H, Zhang Y, Feng L, Zhang C, et al. TSPAN4-positive migrasome derived from retinal pigmented epithelium cells contributes to the development of proliferative vitreoretinopathy. J Nanobiotechnology. 2022;20:519 pubmed publisher
	Hu X, Binter M, Hufendiek K, Tode J, Framme C, Fuchs H. MiR-302d inhibits TGFB-induced EMT and promotes MET in primary human RPE cells. PLoS ONE. 2022;17:e0278158 pubmed publisher
	Jahagirdar D, Yadav S, Gore M, Korpale V, Mathpati C, Chidambaram S, et al. Compartmentalized microfluidic device for in vitro co-culture of retinal cells. Biotechnol J. 2022;17:e2100530 pubmed publisher
	Gallo R, Qureshi F, Strong T, Lang S, Pino K, Dvoriantchikova G, et al. Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines. Transl Vis Sci Technol. 2022;11:4 pubmed publisher
	Wang Y, Chang T, Wu T, Ye W, Wang Y, Dou G, et al. Connective tissue growth factor promotes retinal pigment epithelium mesenchymal transition via the PI3K/AKT signaling pathway. Mol Med Rep. 2021;23: pubmed publisher
	Saddala M, Lennikov A, Mukwaya A, Huang H. Transcriptome-Wide Analysis of CXCR5 Deficient Retinal Pigment Epithelial (RPE) Cells Reveals Molecular Signatures of RPE Homeostasis. Biomedicines. 2020;8: pubmed publisher
	Lyu Q, Ludwig I, Kooten P, Sijts A, Rutten V, van Eden W, et al. Leucinostatin acts as a co-inducer for heat shock protein 70 in cultured canine retinal pigment epithelial cells. Cell Stress Chaperones. 2020;25:235-243 pubmed publisher

image

image 1 :

RPE65 detected in bovine and human samples. Lane 1: bovine RPE membrane, lane 2: recombinant human RPE transfected COS7 cell lysate.

image 2 :

Immunohistochemical staining of RPE65 in mouse retina tissue using MA1-16578.

product information

Product Type :

Antibody

Product Name :

RPE65 Monoclonal Antibody (401.8B11.3D9)

Catalog # :

MA1-16578

Quantity :

200 µL

Price :

US 450.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Canine, Chicken, Human, Mouse, Non-human primate, Porcine, Rat, Xenopus laevis

Applications :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Frozen): 1:250, Immunohistochemistry (Paraffin): 1:250-1:500, Western Blot: 1-2 µg/mL

Species :

Bovine, Canine, Chicken, Human, Mouse, Non-human primate, Porcine, Rat, Xenopus laevis

Clone :

401.8B11.3D9

Isotype :

IgG1, kappa

Storage :

-20°C or -80°C if preferred

Description :

RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.

Immunogen :

Bovine RPE65 microsomal membrane proteins.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Frozen): 1:250, Immunohistochemistry (Paraffin): 1:250-1:500, Western Blot: 1-2 µg/mL

Aliases :

65kDa; A930029L06Rik; all-trans-retinyl-palmitate hydrolase; BCO family, member 3; BCO3; LCA2; Lutein isomerase; membrane receptor p63; meso-zeaxanthin isomerase; modifier of retinal degeneration 1; Mord1; mRPE65; p63; RBP-binding membrane protein; rd12; retinal pigment epithelium 65; retinal pigment epithelium 65-like; retinal pigment epithelium abundant protein RPE65; retinal pigment epithelium specific protein 65; retinal pigment epithelium, 65 kDa; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific 65kD protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; retinal pigment epithelium-specific protein RPE65; Retinitis pigmentos; retinitis pigmentosa 20 (autosomal recessive); retinoid isomerohydrolase; retinoid isomerohydrolase RPE65; retinol isomerase; RP20; RPE65; rpe65 gene for retinal pigment epithelium-specific protein; RPE65, retinoid isomerohydrolase; sRPE65; truncated RPE65

more info or order :

Invitrogen product webpage