HIF-1 beta Monoclonal Antibody (H1beta234) | Invitrogen MA1-16512 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF-1 beta Monoclonal Antibody (H1beta234)

catalog :

MA1-16512

quantity :

100 µL

price :

US 436.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H1beta234

reactivity :

human, mouse, rat, bovine, domestic sheep

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; rat	Maloyan A, Eli Berchoer L, Semenza G, Gerstenblith G, Stern M, Horowitz M. HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart. Physiol Genomics. 2005;23:79-88 pubmed
	Miyazaki K, Kawamoto T, Tanimoto K, Nishiyama M, Honda H, Kato Y. Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes. J Biol Chem. 2002;277:47014-21 pubmed

image

image 1 :

Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.

product information

Product Type :

Antibody

Product Name :

HIF-1 beta Monoclonal Antibody (H1beta234)

Catalog # :

MA1-16512

Quantity :

100 µL

Price :

US 436.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Ferret, Human, Mouse, Non-human primate, Ovine, Rat

Applications :

ChIP Assay: 1:10-1:500, ChIP-Sequencing: Assay-Dependent, Gel Shift: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: Assay-Dependent, Western Blot: 1:500

Species :

Bovine, Ferret, Human, Mouse, Non-human primate, Ovine, Rat

Clone :

H1beta234

Isotype :

IgG1, kappa

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.

Immunogen :

Fusion protein containing residues 496-789 of human ARNT.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AHA-1; ARNT; ARNT protein; Arnt1; ARNT1a; ARNT1b; aryl hydrocarbon receptor nuclear translocater; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; aryl hydrocarbon receptor nuclear translocator type 1a; aryl hydrocarbon receptor nuclear translocator type 1b; bHLHe2; Class E basic helix-loop-helix protein 2; D3Ertd557e; dioxin receptor, nuclear translocator; Drnt; ESTM42; hif 1; HIF1 beta; HIF-1 beta; Hif1b; HIF1BETA; HIF-1beta; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; hypoxia-inducible factor 1-beta; mKIAA4051; TANGO; W08714; zgc:136664

more info or order :

Invitrogen product webpage