HIF1A Monoclonal Antibody (H1alpha67) | Invitrogen MA1-16511 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF1A Monoclonal Antibody (H1alpha67)

catalog :

MA1-16511

quantity :

100 µL

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

H1alpha67

reactivity :

human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 53

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 1b	Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed publisher
immunohistochemistry; pigs ; 1:1000; loading ...; tbl 1	Arvola O, Haapanen H, Herajärvi J, Anttila T, Puistola U, Karihtala P, et al. Remote Ischemic Preconditioning Reduces Cerebral Oxidative Stress Following Hypothermic Circulatory Arrest in a Porcine Model. Semin Thorac Cardiovasc Surg. 2016;28:92-102 pubmed publisher
immunohistochemistry; human; 1:200	BÃ¡nfi G, Teleki I, NyirÃ¡dy P, Keszthelyi A, Romics I, Fintha A, et al. Changes of protein expression in prostate cancer having lost its androgen sensitivity. Int Urol Nephrol. 2015;47:1149-54 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50	Schreurs L, Smit J, Pavlov K, Pultrum B, Pruim J, Groen H, et al. Prognostic impact of clinicopathological features and expression of biomarkers related to (18)F-FDG uptake in esophageal cancer. Ann Surg Oncol. 2014;21:3751-7 pubmed publisher
immunohistochemistry; human	Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, et al. The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma. BMC Cancer. 2014;14:149 pubmed publisher
immunohistochemistry; human; 1:10,000	Choueiri T, Fay A, Gagnon R, Lin Y, Bahamón B, Brown V, et al. The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma. Clin Cancer Res. 2013;19:5218-26 pubmed publisher
immunohistochemistry; human; 1:100	van den Berg L, Crane L, van Oosten M, van Dam G, Simons A, Hofker H, et al. Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging. Int J Gynaecol Obstet. 2013;121:35-40 pubmed publisher
immunohistochemistry; human; 1:50	Park J, Jin Y, Lee Y, Lee J, Lee Y, Ko Y, et al. KAI1 suppresses HIF-1? and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer. BMC Cancer. 2012;12:81 pubmed publisher
immunohistochemistry; rat; 1:200	Hsieh Y, Chou L, Chang P, Yang C, Kao M, Hong C. Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?). J Comp Neurol. 2012;520:2903-16 pubmed publisher
immunohistochemistry; human	Facciabene A, Peng X, Hagemann I, Balint K, Barchetti A, Wang L, et al. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature. 2011;475:226-30 pubmed publisher
immunohistochemistry; mouse	Ahn J, Joo H, Suh J, Kim H, So H, Chung J. Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice. Otol Neurotol. 2011;32:926-32 pubmed publisher
immunohistochemistry; human	Takagi Y, Kikuta K, Moriwaki T, Aoki T, Nozaki K, Hashimoto N, et al. Expression of thioredoxin-1 and hypoxia inducible factor-1? in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property. Surg Neurol Int. 2011;2:61 pubmed publisher
immunohistochemistry; human; 1:20	Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, et al. Immunohistochemical study of the angiogenetic network of VEGF, HIF1?, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer. Pathol Oncol Res. 2012;18:33-41 pubmed publisher
immunohistochemistry; human; 1:20	Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y. Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1? accumulation via phosphatidylinositol 3-kinase/Akt pathway. J Pharmacol Exp Ther. 2011;338:485-91 pubmed publisher
immunohistochemistry; mouse	Qing G, Skuli N, Mayes P, Pawel B, Martinez D, Maris J, et al. Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha. Cancer Res. 2010;70:10351-61 pubmed publisher
immunohistochemistry; human; 1:50	Takala H, Saarnio J, Wiik H, Ohtonen P, Soini Y. HIF-1? and VEGF are associated with disease progression in esophageal carcinoma. J Surg Res. 2011;167:41-8 pubmed publisher
western blot; human	Sumiyoshi M, Kimura Y. Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism. Phytomedicine. 2010;17:820-9 pubmed publisher
western blot; rat	Ali M, Janic B, Babajani Feremi A, Varma N, Iskander A, Anagli J, et al. Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma. PLoS ONE. 2010;5:e8727 pubmed publisher
immunohistochemistry; human; 1:100	Puputti M, Tynninen O, Pernilä P, Salmi M, Jalkanen S, Paetau A, et al. Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors. Brain Pathol. 2010;20:763-70 pubmed publisher
immunohistochemistry; human	Li M, Gu Y, Zhang Z, Zhang S, Zhang D, Saleem A, et al. Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma. Pathol Oncol Res. 2010;16:259-66 pubmed publisher
western blot; mouse	Matsumoto K, Obara N, Ema M, Horie M, Naka A, Takahashi S, et al. Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model. Cancer Sci. 2009;100:1639-47 pubmed publisher
immunohistochemistry; human	Deniz H, Karakok M, Yagci F, Guldur M. Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas. Int Urol Nephrol. 2010;42:103-7 pubmed publisher
immunohistochemistry; mouse; 1:50	Bailey S, Mantena S, Millender Swain T, Cakir Y, Jhala N, Chhieng D, et al. Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease. Free Radic Biol Med. 2009;46:928-38 pubmed
western blot; human	Kang M, Jung S, Kim M, Bae J, Kim H, Kim J, et al. DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells. FEBS J. 2008;275:5969-81 pubmed publisher
immunohistochemistry; human	Arbab A, Janic B, Knight R, Anderson S, Pawelczyk E, Rad A, et al. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. FASEB J. 2008;22:3234-46 pubmed publisher
western blot; mouse; 1:200 western blot; human; 1:200	Rodríguez Jiménez F, Moreno Manzano V, Lucas Dominguez R, Sánchez Puelles J. Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells. Stem Cells. 2008;26:2052-62 pubmed publisher
immunohistochemistry; human; 1:50	Vakkala M, Laurila J, Saarnio J, Koivukangas V, Syrjala H, Karttunen T, et al. Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis. Crit Care. 2007;11:R116 pubmed
immunocytochemistry; rat; 1:200	Agrawal A, Guttapalli A, Narayan S, Albert T, Shapiro I, Risbud M. Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol. 2007;293:C621-31 pubmed
immunohistochemistry; human; 1:100	Sihto H, Tynninen O, Butzow R, Saarialho Kere U, Joensuu H. Endothelial cell KIT expression in human tumours. J Pathol. 2007;211:481-8 pubmed
immunohistochemistry; human	Zagzag D, Lukyanov Y, Lan L, Ali M, Esencay M, Mendez O, et al. Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion. Lab Invest. 2006;86:1221-32 pubmed
western blot; human; 1:1000	Pore N, Jiang Z, Shu H, Bernhard E, Kao G, Maity A. Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway. Mol Cancer Res. 2006;4:471-9 pubmed
immunohistochemistry; domestic rabbit; 1:4000	Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C. Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system. J Orthop Res. 2006;24:653-63 pubmed
immunocytochemistry; human; 1:200	Risbud M, Guttapalli A, Stokes D, Hawkins D, Danielson K, Schaer T, et al. Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem. 2006;98:152-9 pubmed
western blot; rat	Maloyan A, Eli Berchoer L, Semenza G, Gerstenblith G, Stern M, Horowitz M. HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart. Physiol Genomics. 2005;23:79-88 pubmed
immunohistochemistry; human	Huang G, Yang L, Lu W. Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival. World J Gastroenterol. 2005;11:1705-8 pubmed
	K xf6 se F, Pabuccuoglu A, Karakoyun M, Aydogdu S. Peroxiredoxins and Hypoxia-Inducible Factor-1α in Duodenal Tissue: Emerging Factors in the Pathophysiology of Pediatric Celiac Disease Patients. Curr Issues Mol Biol. 2023;45:1779-1793 pubmed publisher
	Chen J, Argemi J, Odena G, Xu M, Cai Y, Massey V, et al. Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension. Sci Rep. 2020;10:15558 pubmed publisher
	Hou P, Chen F, Yong H, Lin T, Li J, Pan Y, et al. PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α. J Exp Clin Cancer Res. 2019;38:301 pubmed publisher
	Hedborg F, Fischer Colbrie R, Ostlin N, Sandstedt B, Tran M, Maxwell P. Differentiation in neuroblastoma: diffusion-limited hypoxia induces neuro-endocrine secretory protein 55 and other markers of a chromaffin phenotype. PLoS ONE. 2010;5: pubmed publisher
	Wang Y, Pakunlu R, Tsao W, Pozharov V, Minko T. Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis. Mol Pharm. 2004;1:156-65 pubmed
	Chung J, Kang H, Shin J, Kim J. Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation. Neuroreport. 2004;15:2353-6 pubmed
	Acs G, Xu X, Chu C, Acs P, Verma A. Prognostic significance of erythropoietin expression in human endometrial carcinoma. Cancer. 2004;100:2376-86 pubmed
	Tuomisto T, Rissanen T, Vajanto I, Korkeela A, Rutanen J, Yla Herttuala S. HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. Atherosclerosis. 2004;174:111-20 pubmed
	Ma W, Tessarollo L, Hong S, Baba M, Southon E, Back T, et al. Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene. Cancer Res. 2003;63:5320-8 pubmed
	Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, et al. Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage. Br J Cancer. 2003;89:1042-7 pubmed
	Mehrabi M, Serbecic N, Tamaddon F, Pacher R, Horvath R, Mall G, et al. Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium. Biomed Pharmacother. 2003;57:173-8 pubmed
	Morita M, Ohneda O, Yamashita T, Takahashi S, Suzuki N, Nakajima O, et al. HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin. EMBO J. 2003;22:1134-46 pubmed
	Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells. Environ Health Perspect. 2002;110 Suppl 5:783-8 pubmed
	Mehrabi M, Serbecic N, Tamaddon F, Kaun C, Huber K, Pacher R, et al. Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease. Cardiovasc Res. 2002;56:214-24 pubmed
	Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis. Cell Biol Toxicol. 2002;18:87-96 pubmed
	Kim M, Kwon H, Lee Y, Baek J, Jang J, Lee S, et al. Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes. Nat Med. 2001;7:437-43 pubmed
	Yamashita K, Discher D, Hu J, Bishopric N, Webster K. Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP. J Biol Chem. 2001;276:12645-53 pubmed
	Salnikow K, Costa M, Figg W, Blagosklonny M. Hyperinducibility of hypoxia-responsive genes without p53/p21-dependent checkpoint in aggressive prostate cancer. Cancer Res. 2000;60:5630-4 pubmed

image

image 1 :

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511.

image 2 :

Immunoprecipitation of HIF-1 alpha; Heavy and light chains are also detected.

image 3 :

Western Blot detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.

product information

Product Type :

Antibody

Product Name :

HIF1A Monoclonal Antibody (H1alpha67)

Catalog # :

MA1-16511

Quantity :

100 µL

Price :

US 450.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Avian, Bovine, Canine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat

Applications :

ChIP Assay: 1:10-1:500, ELISA: 1:100-1:2,000, Flow Cytometry: 1:10-1:1,000, Gel Shift: 1:1-1:100, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 1:100-1:300, Immunoprecipitation: 1:10, Western Blot: 1:500-1:1,000

Species :

Avian, Bovine, Canine, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat

Clone :

H1alpha67

Isotype :

IgG2b

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Immunogen :

Fusion protein containing residues 432-528 of human HIF-1alpha

Format :

Liquid

Applications w/Dilutions :

Aliases :

AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

more info or order :

Invitrogen product webpage