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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
beta Actin Monoclonal Antibody (15G5A11/E2)
catalog :
MA1-140
quantity :
100 µg
price :
US 385.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
15G5A11/E2
reactivity :
human, mouse, rat, dogs
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
citations: 57
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:10,000; fig 5
Yao Y, Wei W, Sun J, Chen L, Deng X, Ma L, et al. Proteomic analysis of exosomes derived from human lymphoma cells. Eur J Med Res. 2015;20:8 pubmed publisher
Schwab K, Coronel L, Riege K, Sacramento E, Rahnis N, H xe4 ckes D, et al. Multi-omics analysis identifies RFX7 targets involved in tumor suppression and neuronal processes. Cell Death Discov. 2023;9:80 pubmed publisher
Sun L, Ding M, Chen F, Zhu D, Xie X. Breviscapine alleviates podocyte injury by inhibiting NF-κB/NLRP3-mediated pyroptosis in diabetic nephropathy. Peerj. 2023;11:e14826 pubmed publisher
Jacksi M, Schad E, Buday L, Tantos A. Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells. Int J Mol Sci. 2023;24: pubmed publisher
Sun B, Zhang Y, Zhang M, Liu R, Yang W. Gene therapy targeting miR‑212‑3p exerts therapeutic effects on MAFLD similar to those of exercise. Int J Mol Med. 2023;51: pubmed publisher
S xe1 nchez J, Mu xf1 oz L, Galindo M xe1 rquez M, Valencia V xe1 squez A, Garc xed a A. Paclitaxel Regulates TRPA1 Function and Expression Through PKA and PKC. Neurochem Res. 2023;48:295-304 pubmed publisher
Muranyi W, Schwerk C, Herold R, Stump Guthier C, Lampe M, Fallier Becker P, et al. Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus. iScience. 2022;25:104383 pubmed publisher
Vanhulle E, Stroobants J, Provinciael B, Camps A, Noppen S, Maes P, et al. SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening. Antiviral Res. 2022;203:105342 pubmed publisher
Gay M, Cao H, Shivapurkar N, Dakshanamurthy S, Kallakury B, Tucker R, et al. Proglumide Reverses Nonalcoholic Steatohepatitis by Interaction with the Farnesoid X Receptor and Altering the Microbiome. Int J Mol Sci. 2022;23: pubmed publisher
Pandya P, Kublo L, Stewart Ornstein J. p53 Promotes Cytokine Expression in Melanoma to Regulate Drug Resistance and Migration. Cells. 2022;11: pubmed publisher
Jang S, Jung B, An Y, Jang H. The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant New castle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse model. Virol J. 2022;19:21 pubmed publisher
Pauwels E, Provinciael B, Camps A, Hartmann E, Vermeire K. Reduced DNAJC3 Expression Affects Protein Translocation across the ER Membrane and Attenuates the Down-Modulating Effect of the Translocation Inhibitor Cyclotriazadisulfonamide. Int J Mol Sci. 2022;23: pubmed publisher
Cluff E, Magdaleno C, Fernandez E, House T, Swaminathan S, Varadaraj A, et al. Hypoxia-inducible factor-1 alpha expression is induced by IL-2 via the PI3K/mTOR pathway in hypoxic NK cells and supports effector functions in NKL cells and ex vivo expanded NK cells. Cancer Immunol Immunother. 2022;: pubmed publisher
Ol xe1 h J, Lehotzky A, Sz xe9 n xe1 si T, Ov xe1 di J. Anti-Aggregative Effect of the Antioxidant DJ-1 on the TPPP/p25-Derived Pathological Associations of Alpha-Synuclein. Cells. 2021;10: pubmed publisher
Zhang H, Wu G, Feng J, Lu X, Liu P. Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1. J Orthop Surg Res. 2021;16:667 pubmed publisher
Malchiodi Z, Cao H, Gay M, Safronenka A, Bansal S, Tucker R, et al. Cholecystokinin Receptor Antagonist Improves Efficacy of Chemotherapy in Murine Models of Pancreatic Cancer by Altering the Tumor Microenvironment. Cancers (Basel). 2021;13: pubmed publisher
Pauwels E, Rutz C, Provinciael B, Stroobants J, Schols D, Hartmann E, et al. A Proteomic Study on the Membrane Protein Fraction of T Cells Confirms High Substrate Selectivity for the ER Translocation Inhibitor Cyclotriazadisulfonamide. Mol Cell Proteomics. 2021;20:100144 pubmed publisher
Ezzeldeen Y, Swidan S, ElMeshad A, Sebak A. Green Synthesized Honokiol Transfersomes Relieve the Immunosuppressive and Stem-Like Cell Characteristics of the Aggressive B16F10 Melanoma. Int J Nanomedicine. 2021;16:5693-5712 pubmed publisher
Pandolfi L, Marengo A, Japiassu K, Frangipane V, Tsapis N, Bincoletto V, et al. Liposomes Loaded with Everolimus and Coated with Hyaluronic Acid: A Promising Approach for Lung Fibrosis. Int J Mol Sci. 2021;22: pubmed publisher
Xu X, Ni K, He Y, Ren J, Sun C, Liu Y, et al. The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation. Nat Commun. 2021;12:3520 pubmed publisher
Miano M, Grossi A, Dell Orso G, Lanciotti M, Fioredda F, Palmisani E, et al. Genetic screening of children with marrow failure. The role of primary Immunodeficiencies. Am J Hematol. 2021;96:1077-1086 pubmed publisher
Bandyopadhyay S, Douglass J, Kapell S, Khan N, Feitosa Suntheimer F, Klein J, et al. DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci. G3 (Bethesda). 2021;: pubmed publisher
Ghosh S, Won S, Wang J, Fong R, Butler N, Moss A, et al. α-synuclein aggregates induce c-Abl activation and dopaminergic neuronal loss by a feed-forward redox stress mechanism. Prog Neurobiol. 2021;202:102070 pubmed publisher
Gupta V, Hirst J, Petersen S, Roby K, Kusch M, Zhou H, et al. Entinostat, a selective HDAC1/2 inhibitor, potentiates the effects of olaparib in homologous recombination proficient ovarian cancer. Gynecol Oncol. 2021;162:163-172 pubmed publisher
Koldobskiy M, Jenkinson G, Abante J, Rodriguez DiBlasi V, Zhou W, Pujadas E, et al. Converging genetic and epigenetic drivers of paediatric acute lymphoblastic leukaemia identified by an information-theoretic analysis. Nat Biomed Eng. 2021;5:360-376 pubmed publisher
Aoto K, Kato M, Akita T, Nakashima M, Mutoh H, Akasaka N, et al. ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H+-ATPases is essential for brain development in humans and mice. Nat Commun. 2021;12:2107 pubmed publisher
Hua Z, Li D, Wu A, Cao T, Luo S. miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus. Open Med (Wars). 2021;16:464-471 pubmed publisher
Sun B, Jiang J, Zhu X, Yang D, Cui Z, Zhang Y, et al. Protective effects of gastrodin pretreatment on mouse hepatic ischemia-reperfusion occurring through antioxidant and anti-apoptotic mechanisms. Exp Ther Med. 2021;21:471 pubmed publisher
Zhang M, Fei Z, Huang L. Micro-RNA-451 Reduces Proliferation of B-CPAP Human Papillary Thyroid Cancer Cells by Downregulating Expression of Activating Transcription Factor 2. Med Sci Monit. 2021;27:e929774 pubmed publisher
Azad T, Singaravelu R, Brown E, Taha Z, Rezaei R, Arulanandam R, et al. SARS-CoV-2 S1 NanoBiT: A nanoluciferase complementation-based biosensor to rapidly probe SARS-CoV-2 receptor recognition. Biosens Bioelectron. 2021;180:113122 pubmed publisher
Villamizar O, Waters S, Scott T, Grepo N, Jaffe A, Morris K. Mesenchymal Stem Cell exosome delivered Zinc Finger Protein activation of cystic fibrosis transmembrane conductance regulator. J Extracell Vesicles. 2021;10:e12053 pubmed publisher
Magdaleno C, House T, Pawar J, Carvalho S, Rajasekaran N, Varadaraj A. Fibronectin assembly regulates lumen formation in breast acini. J Cell Biochem. 2021;122:524-537 pubmed publisher
Sztretye M, Singlár Z, Balogh N, Kis G, Szentesi P, Angyal Á, et al. The Role of Orai1 in Regulating Sarcoplasmic Calcium Release, Mitochondrial Morphology and Function in Myostatin Deficient Skeletal Muscle. Front Physiol. 2020;11:601090 pubmed publisher
Liu Q, Xie Z, Qiu M, Shim I, Yang Y, Xie S, et al. Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer. Adv Sci (Weinh). 2020;7:2001191 pubmed publisher
Sanders J, Freeman T, Xu Y, Golloshi R, Stallard M, Hill A, et al. Radiation-induced DNA damage and repair effects on 3D genome organization. Nat Commun. 2020;11:6178 pubmed publisher
Pandolfi L, Fusco R, Frangipane V, D Amico R, Giustra M, Bozzini S, et al. Loading Imatinib inside targeted nanoparticles to prevent Bronchiolitis Obliterans Syndrome. Sci Rep. 2020;10:20726 pubmed publisher
Magdaleno C, Dixon L, Rajasekaran N, Varadaraj A. HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl2. Sci Rep. 2020;10:18560 pubmed publisher
Yang L, Slone J, Zou W, Queme L, Jankowski M, Yin F, et al. Systemic Delivery of AAV-Fdxr Mitigates the Phenotypes of Mitochondrial Disorders in Fdxr Mutant Mice. Mol Ther Methods Clin Dev. 2020;18:84-97 pubmed publisher
Liu B, Shen Y, Huang H, Croce K, Wu M, Fan Y, et al. Curcumin derivative C212 inhibits Hsp90 and eliminates both growing and quiescent leukemia cells in deep dormancy. Cell Commun Signal. 2020;18:159 pubmed publisher
Makinwa Y, Cartwright B, Musich P, Li Z, Biswas H, Zou Y. PP2A Regulates Phosphorylation-Dependent Isomerization of Cytoplasmic and Mitochondrial-Associated ATR by Pin1 in DNA Damage Responses. Front Cell Dev Biol. 2020;8:813 pubmed publisher
Fefilova A, Melnikov P, Prikazchikova T, Abakumova T, Kurochkin I, Mazin P, et al. Murine Long Noncoding RNA Morrbid Contributes in the Regulation of NRAS Splicing in Hepatocytes In Vitro. Int J Mol Sci. 2020;21: pubmed publisher
Dibitetto D, Sims J, Ascenção C, Feng K, Kim D, Oberly S, et al. Intrinsic ATR signaling shapes DNA end resection and suppresses toxic DNA-PKcs signaling. NAR Cancer. 2020;2:zcaa006 pubmed publisher
Besson B, Basset J, Gatellier S, Chabrolles H, Chaze T, Hourdel V, et al. Comparison of a human neuronal model proteome upon Japanese encephalitis or West Nile Virus infection and potential role of mosquito saliva in neuropathogenesis. PLoS ONE. 2020;15:e0232585 pubmed publisher
Sergeeva O, Sergeev P, Melnikov P, Prikazchikova T, Dontsova O, Zatsepin T. Modification of Adenosine196 by Mettl3 Methyltransferase in the 5'-External Transcribed Spacer of 47S Pre-rRNA Affects rRNA Maturation. Cells. 2020;9: pubmed publisher
Kumai A, Tsugami Y, Wakasa H, Suzuki N, Suzuki T, Nishimura T, et al. Adverse Effects of Coumestrol and Genistein on Mammary Morphogenesis and Future Milk Production Ability of Mammary Epithelial Cells. Adv Biosyst. 2020;4:e1900187 pubmed publisher
Kobayashi K, Tsugami Y, Suzuki N, Suzuki T, Nishimura T. Nicotine directly affects milk production in lactating mammary epithelial cells concurrently with inactivation of STAT5 and glucocorticoid receptor in vitro. Toxicol In Vitro. 2020;63:104741 pubmed publisher
Lloyd J, Copaciu R, Yahyabeik A, DeWit C, Cummings K, Lacey M, et al. Characterization of polyclonal antibodies to Herpes Simplex Virus types 1 and 2. J Histotechnol. 2019;42:202-214 pubmed publisher
Osborne N, Sundseth R, Gay M, Cao H, Tucker R, Nadella S, et al. Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases. Am J Physiol Gastrointest Liver Physiol. 2019;317:G682-G693 pubmed publisher
Tucker R, Ciofoaia V, Nadella S, Gay M, Cao H, Huber M, et al. A Cholecystokinin Receptor Antagonist Halts Nonalcoholic Steatohepatitis and Prevents Hepatocellular Carcinoma. Dig Dis Sci. 2020;65:189-203 pubmed publisher
Chen T, Lee R, Tsai S, Kanamaluru D, Gray N, Yiv N, et al. An ANGPTL4-ceramide-protein kinase Cζ axis mediates chronic glucocorticoid exposure-induced hepatic steatosis and hypertriglyceridemia in mice. J Biol Chem. 2019;294:9213-9224 pubmed publisher
Schmit N, Neopane K, Hantschel O. Targeted Protein Degradation through Cytosolic Delivery of Monobody Binders Using Bacterial Toxins. ACS Chem Biol. 2019;14:916-924 pubmed publisher
Chen L, Zhang J, Suo H, Wang W, Wang H, Zhang Y, et al. Preventive Effects of Different Fermentation Times of Shuidouchi on Diphenoxylate-Induced Constipation in Mice. Foods. 2019;8: pubmed publisher
Wang R, Zhang Z, Kumar M, Xu G, Zhang M. Neuroprotective potential of ketamine prevents developing brain structure impairment and alteration of neurocognitive function induced via isoflurane through the PI3K/AKT/GSK-3β pathway. Drug Des Devel Ther. 2019;13:501-512 pubmed publisher
Jiao H, Yan Z, Ma Q, Li X, Jiang Y, Liu Y, et al. Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus. Neuropsychiatr Dis Treat. 2019;15:21-31 pubmed publisher
Liu Q, Fan D, Adah D, Wu Z, Liu R, Yan Q, et al. CRISPR/Cas9‑mediated hypoxia inducible factor‑1α knockout enhances the antitumor effect of transarterial embolization in hepatocellular carcinoma. Oncol Rep. 2018;40:2547-2557 pubmed publisher
Qian Y, Song J, Sun P, Yi R, Liu H, Feng X, et al. Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells. Molecules. 2018;23: pubmed publisher
Daly C, Flemban A, Shafei M, Conway M, Qualtrough D, Dean S. Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line. Oncol Rep. 2017;: pubmed publisher
product information
Product Type :
Antibody
Product Name :
beta Actin Monoclonal Antibody (15G5A11/E2)
Catalog # :
MA1-140
Quantity :
100 µg
Price :
US 385.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Canine, Human, Mouse, Non-human primate, Rat
Applications :
Immunocytochemistry: 1:1,000-1:4,000, Immunoprecipitation: 2 µg, Western Blot: 1:5,000-1:20,000
Species :
Canine, Human, Mouse, Non-human primate, Rat
Clone :
15G5A11/E2
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Immunogen :
Purified full-length recombinant human beta-actin expressed in E. coli
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:1,000-1:4,000, Immunoprecipitation: 2 µg, Western Blot: 1:5,000-1:20,000
Aliases :
0610041G09Rik; AA959943; AAT6; ACT; Act4; Act-4; ACT-5; ACTA; acta1; acta1b; ACTA2; Acta-2; ACTA3; actb; actb.L; actb1; actba; ACTC; actc1; Actc-1; actc1b; ACTE; Actg; ACTG1; Actg2; ACTGE; actin; actin alpha 1; actin alpha 1, skeletal muscle; actin alpha 1, skeletal muscle b; actin alpha 2, smooth muscle; actin alpha cardiac; actin alpha cardiac 1; actin alpha cardiac muscle 1; actin alpha cardiac muscle 1b; actin beta; actin gamma 1; actin gamma 2, smooth muscle; actin, alpha 1, skeletal muscle; actin, alpha 1b, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; actin, alpha cardiac muscle 1b; actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, cardiac muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; actin, beta 1; actin, beta L homeolog; actin, beta, cytoplasmic; actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma 1; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; Actx; AL023024; alpha actin 1; alpha-actin cardiac; alpha-actin-1; Alpha-actin-2; alpha-actin-3; alphac-actin; Alpha-cardiac actin; alphaSMA; alpha-smooth muscle actin; ASD5; ASMA; a-SMA; Bact; Bact; actin; B-actin; bactin1; bactin1 protein; bactzf; B-ACTZF; beta actin; beta cytoskeletal actin; beta-actin; beta-actin FE-3; beta-actin-1; BRWS1; BRWS2; cardiac muscle alpha actin 1; cardiofunk; cell growth-inhibiting gene 46 protein; Cfk; CFTD; CFTD1; CFTDM; CMD1R; CMH11; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeletal gamma-actin; cytoskeletal protein; deafness, autosomal dominant 20; deafness, autosomal dominant 26; DFNA20; DFNA26; E430023M04Rik; E51; epididymis luminal protein 176; fa27h01; fb83f06; gamma non-muscle actin; gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; HEL-176; hm:zeh0631; I79_002310; I79_013242; I79_019066; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; nemaline myopathy type 3; PS1TP5-binding protein 1; PS1TP5BP1; SHPM; similar to beta actin; skeletal alpha actin; skeletal alpha1 actin; sma; SMalphaA; SMGA; smooth muscle alpha-actin; smooth muscle gamma-actin; vascular smooth muscle alpha-actin; VSCM; wu:fa27h01; wu:fb63d03; wu:fb83f06; wu:fd18f05; XELAEV_18045052mg; zeh0631
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA