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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
catalog :
MA1-135
quantity :
100 µg
price :
US 269.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4E3D10H2/E3
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
more info or order :
citations: 35
Reference
Guo Q, Xin M, Lu Q, Feng D, Yang V, Peng L, et al. A novel NEDD4L-TXNIP-CHOP axis in the pathogenesis of nonalcoholic steatohepatitis. Theranostics. 2023;13:2210-2225 pubmed publisher
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Kelkka T, Tyster M, Lundgren S, Feng X, Kerr C, Hosokawa K, et al. Anti-COX-2 autoantibody is a novel biomarker of immune aplastic anemia. Leukemia. 2022;36:2317-2327 pubmed publisher
Menzies S, Dawson C, Crittenden E, Edge R, Hall S, Alsolaiss J, et al. Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition. Sci Rep. 2022;12:11328 pubmed publisher
Chen W, Wilson M, Wang Y, Bergmeier W, Lanza F, Li R. Fast clearance of platelets in a commonly used mouse model for GPIbα is impeded by an anti-GPIbβ antibody derivative. J Thromb Haemost. 2022;20:1451-1463 pubmed publisher
Gui L, Zhang S, Xu Y, Zhang H, Zhu Y, Kong L. UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma. Cell Death Discov. 2021;7:357 pubmed publisher
Dai S, Liu F, Ren M, Qin Z, Rout N, Yang X, et al. Complement Inhibition Targeted to Injury Specific Neoepitopes Attenuates Atherogenesis in Mice. Front Cardiovasc Med. 2021;8:731315 pubmed publisher
Song X, Cronan J. A conserved and seemingly redundant Escherichia coli biotin biosynthesis gene expressed only during anaerobic growth. Mol Microbiol. 2021;116:1315-1327 pubmed publisher
Pandey S, Singh A, Yang G, d Andrea F, Jiang X, Hartman T, et al. Characterization of Phosphopantetheinyl Hydrolase from Mycobacterium tuberculosis. Microbiol Spectr. 2021;9:e0092821 pubmed publisher
Guerrero Santoro J, Khor J, A xe7 x131 kba x15f A, Jaynes J, Ettensohn C. Analysis of the DNA-binding properties of Alx1, an evolutionarily conserved regulator of skeletogenesis in echinoderms. J Biol Chem. 2021;297:100901 pubmed publisher
Bamra T, Shafi T, Das S, Kumar M, Dikhit M, Kumar A, et al. Leishmania donovani Secretory Mevalonate Kinase Regulates Host Immune Response and Facilitates Phagocytosis. Front Cell Infect Microbiol. 2021;11:641985 pubmed publisher
Lin B, Phung T, Higgins N, Greenslade J, Prado M, Finley D, et al. ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import. Hum Mol Genet. 2021;30:1230-1246 pubmed publisher
Schneid S, Wolff F, Buchner K, Bertram N, Bayg xfc n S, Barbosa P, et al. The BRCT domains of ECT2 have distinct functions during cytokinesis. Cell Rep. 2021;34:108805 pubmed publisher
Großkopf A, Schlagowski S, Fricke T, Ensser A, Desrosiers R, Hahn A. Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV). PLoS Pathog. 2021;17:e1008979 pubmed publisher
Muñoz Alía M, Nace R, Tischer A, Zhang L, Bah E, Auton M, et al. Mev-stealth: A cd46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum. PLoS Pathog. 2021;17:e1009283 pubmed publisher
Gupta A, Arora G, Rosen C, Kloos Z, Cao Y, Cerny J, et al. A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLoS Pathog. 2020;16:e1009030 pubmed publisher
Jang J, Nguyen N, Légeret B, Beisson F, Kim Y, Sim H, et al. Phospholipase pPLAIIIα Increases Germination Rate and Resistance to Turnip Crinkle Virus when Overexpressed. Plant Physiol. 2020;184:1482-1498 pubmed publisher
Kimura S, Hunter K, Vaahtera L, Tran H, Citterico M, Vaattovaara A, et al. CRK2 and C-terminal Phosphorylation of NADPH Oxidase RBOHD Regulate Reactive Oxygen Species Production in Arabidopsis. Plant Cell. 2020;32:1063-1080 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
Catalog # :
MA1-135
Quantity :
100 µg
Price :
US 269.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Tag
Applications :
ELISA: 25-500 ng/mL, Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg, Western Blot: 1:250-1:1,000
Species :
Tag
Clone :
4E3D10H2/E3
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC
Format :
Liquid
Applications w/Dilutions :
ELISA: 25-500 ng/mL, Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg, Western Blot: 1:250-1:1,000
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA