GLO1 Monoclonal Antibody (Glo1a) | Invitrogen MA1-13029 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GLO1 Monoclonal Antibody (Glo1a)

catalog :

MA1-13029

quantity :

100 µg

price :

US 486

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Glo1a

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 1d	Qi W, Keenan H, Li Q, Ishikado A, Kannt A, Sadowski T, et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med. 2017;23:753-762 pubmed publisher
	Palmerini M, Macchiarelli G, Cocciolone D, Mascitti I, Placidi M, Vergara T, et al. Modulating Morphological and Redox/Glycative Alterations in the PCOS Uterus: Effects of Carnitines in PCOS Mice. Biomedicines. 2023;11: pubmed publisher
	Stratmann B, Eggers B, Mattern Y, Silva de Carvalho T, Marcus K, Tschoepe D. Chronic Hyperglycaemia Inhibits Tricarboxylic Acid Cycle in Rat Cardiomyoblasts Overexpressing Glucose Transporter Type 4. Int J Mol Sci. 2022;23: pubmed publisher
	Emidio G, Placidi M, Rea F, Rossi G, Falone S, Cristiano L, et al. Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice. Cells. 2020;9: pubmed publisher
	Michel M, Hollenbach M, Pohl S, Ripoll C, Zipprich A. Inhibition of Glyoxalase-I Leads to Reduced Proliferation, Migration and Colony Formation, and Enhanced Susceptibility to Sorafenib in Hepatocellular Carcinoma. Front Oncol. 2019;9:785 pubmed publisher
	Wang Z, Zhang J, Chen L, Li J, Zhang H, Guo X. Glycine Suppresses AGE/RAGE Signaling Pathway and Subsequent Oxidative Stress by Restoring Glo1 Function in the Aorta of Diabetic Rats and in HUVECs. Oxid Med Cell Longev. 2019;2019:4628962 pubmed publisher
	Wang Z, Zhao D, Chen L, Li J, Yuan G, Yang G, et al. Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats. J Diabetes Investig. 2019;10:1189-1198 pubmed publisher

image

image 1 :

Western blot analysis of Glyoxylase (Glo1) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a Goat anti-Mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077). For siRNA knock-down experiments HeLa and U20S cells were treated with 100nM Glo1 siRNA or control siRNA for 48 hours. Lysates were collected and Western blot analysis performed as described above.

image 2 :

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:800 with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin. Images are displayed at 40X magnification. Results demonstrate cytoplasmic localization of Glyoxylase.

product information

Product Type :

Antibody

Product Name :

GLO1 Monoclonal Antibody (Glo1a)

Catalog # :

MA1-13029

Quantity :

100 µg

Price :

US 486

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunohistochemistry (Paraffin): 1:800, Western Blot: 1:1,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

Glo1a

Isotype :

IgG

Storage :

-20°C

Description :

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Immunogen :

Recombinant human GLOI-GST fusion protein.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:800, Western Blot: 1:1,000

Aliases :

0610009E22Rik; 1110008E19Rik; 2510049H23Rik; aldoketomutase; AW550643; cb554; epididymis secretory protein Li 74; GLO1; Glo-1; Glo-1r; Glo1-r; Glo-1s; Glo1-s; GLOD1; glx I; GLY1; GLYI; glyoxalase 1; glyoxalase 1 complex; glyoxalase 1 regulatory; glyoxalase 1 structural; glyoxalase domain containing 1; glyoxalase I; glyoxylase 1; HEL-S-74; ketone-aldehyde mutase; lactoyl glutathione lyase; lactoylglutathione lyase; lactoylglutathione lyase GLO1; methylglyoxalase; Qglo; S-D-lactoylglutathione methylglyoxal lyase; wu:fb82g09; YM9571.15C; YML004C; zgc:66035

more info or order :

Invitrogen product webpage