ZPR1 Monoclonal Antibody (LG1) | Invitrogen MA1-13003 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ZPR1 Monoclonal Antibody (LG1)

catalog :

MA1-13003

quantity :

100 µg

price :

US 435

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LG1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse	Gangwani L, Flavell R, Davis R. ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies. Mol Cell Biol. 2005;25:2744-56 pubmed
	Rademacher S, Detering N, Sch xfc ning T, Lindner R, Santonicola P, Wefel I, et al. A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN. Cells. 2020;9: pubmed publisher
	Ito Y, Smith A, Kernohan K, Pena I, Ahmed A, McDonell L, et al. A ZPR1 mutation is associated with a novel syndrome of growth restriction, distinct craniofacial features, alopecia, and hypoplastic kidneys. Clin Genet. 2018;94:303-312 pubmed publisher
	Doran B, Gherbesi N, Hendricks G, Flavell R, Davis R, Gangwani L. Deficiency of the zinc finger protein ZPR1 causes neurodegeneration. Proc Natl Acad Sci U S A. 2006;103:7471-5 pubmed
	Narayanan U, Achsel T, Luhrmann R, Matera A. Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein. Mol Cell. 2004;16:223-34 pubmed
	Gangwani L, Mikrut M, Theroux S, Sharma M, Davis R. Spinal muscular atrophy disrupts the interaction of ZPR1 with the SMN protein. Nat Cell Biol. 2001;3:376-83 pubmed

image

image 1 :

Immunofluorescent analysis of ZPR1 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ZPR1 monoclonal antibody (Product # MA1-13003) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). ZPR1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of ZPR1 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ZPR1 monoclonal antibody (Product # MA1-13003) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). ZPR1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

ZPR1 Monoclonal Antibody (LG1)

Catalog # :

MA1-13003

Quantity :

100 µg

Price :

US 435

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1:500, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

LG1

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The protein encoded by this gene is found in the cytoplasm of quiescent cells but translocates to the nucleolus in proliferating cells. The encoded protein interacts with survival motor neuron protein (SMN1) to enhance pre-mRNA splicing and to induce neuronal differentiation and axonal growth. Defects in this gene or the SMN1 gene can cause spinal muscular atrophy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Immunogen :

murine ZPR1 protein

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:500, Western Blot: 1:500-1:2,000

Aliases :

AI303781; fc17g10; hypothetical protein LOC504523; RGD1562173; si:rp71-56i13.4; wu:fc17g10; Zfp259; zgc:56478; Zinc finger protein 259; zinc finger protein ZPR1; ZNF259; Zpr1; ZPR1 zinc finger

more info or order :

Invitrogen product webpage