Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11) | Invitrogen MA1-12692 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)

catalog :

MA1-12692

quantity :

100 µg

price :

US 662

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TE111.5D11

reactivity :

Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , domestic sheep, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 71

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s5c	Aiello A, Bacci L, Re A, Ripoli C, Pierconti F, Pinto F, et al. MALAT1 and HOTAIR Long Non-Coding RNAs Play Opposite Role in Estrogen-Mediated Transcriptional Regulation in Prostate Cancer Cells. Sci Rep. 2016;6:38414 pubmed publisher
western blot; mouse; loading ...; fig 1d immunohistochemistry - frozen section; human; loading ...; fig 1a	Svoronos N, Perales Puchalt A, Allegrezza M, Rutkowski M, Payne K, Tesone A, et al. Tumor Cell-Independent Estrogen Signaling Drives Disease Progression through Mobilization of Myeloid-Derived Suppressor Cells. Cancer Discov. 2017;7:72-85 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Nanbo A, Kachi K, Yoshiyama H, Ohba Y. Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse. J Gen Virol. 2016;97:2989-3006 pubmed publisher
chromatin immunoprecipitation; mouse; fig 1a	Garcia Cuellar M, Büttner C, Bartenhagen C, Dugas M, Slany R. Leukemogenic MLL-ENL Fusions Induce Alternative Chromatin States to Drive a Functionally Dichotomous Group of Target Genes. Cell Rep. 2016;15:310-22 pubmed publisher
immunohistochemistry - paraffin section; chicken; 4 ug/ml; fig 3	GonzÃ¡lez MorÃ¡n M. Immunohistochemical localization of progesterone receptor isoforms and estrogen receptor alpha in the chicken oviduct magnum during development. Acta Histochem. 2015;117:681-7 pubmed publisher
chromatin immunoprecipitation; mouse; fig 1h western blot; mouse; fig 1d	Slaats G, Saldivar J, Bacal J, Zeman M, Kile A, Hynes A, et al. DNA replication stress underlies renal phenotypes in CEP290-associated Joubert syndrome. J Clin Invest. 2015;125:3657-66 pubmed publisher
chromatin immunoprecipitation; mouse; fig 1h western blot; mouse; fig 1d	Malaterre J, Pereira L, Putoczki T, Millen R, Paquet Fifield S, Germann M, et al. Intestinal-specific activatable Myb initiates colon tumorigenesis in mice. Oncogene. 2016;35:2475-84 pubmed publisher
immunohistochemistry; chicken; 4 ug/ml	González Morán M. Changes in the cellular localization of estrogen receptor alpha in the growing and regressing ovaries of Gallus domesticus during development. Biochem Biophys Res Commun. 2014;447:197-204 pubmed publisher
chromatin immunoprecipitation; mouse	Gagnidze K, Weil Z, Faustino L, Schaafsma S, Pfaff D. Early histone modifications in the ventromedial hypothalamus and preoptic area following oestradiol administration. J Neuroendocrinol. 2013;25:939-55 pubmed publisher
EMSA; human	Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
chromatin immunoprecipitation; mouse	Miranda T, Voss T, Sung M, Baek S, John S, HAWKINS M, et al. Reprogramming the chromatin landscape: interplay of the estrogen and glucocorticoid receptors at the genomic level. Cancer Res. 2013;73:5130-9 pubmed publisher
chromatin immunoprecipitation; human	Bhat Nakshatri P, Song E, Collins N, Uversky V, Dunker A, O Malley B, et al. Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing. BMC Med Genomics. 2013;6:21 pubmed publisher
western blot; human; 1:200	Arao Y, Hamilton K, Coons L, Korach K. Estrogen receptor ? L543A,L544A mutation changes antagonists to agonists, correlating with the ligand binding domain dimerization associated with DNA binding activity. J Biol Chem. 2013;288:21105-16 pubmed publisher
chromatin immunoprecipitation; mouse	Maethner E, Garcia Cuellar M, Breitinger C, Takacova S, Divoky V, Hess J, et al. MLL-ENL inhibits polycomb repressive complex 1 to achieve efficient transformation of hematopoietic cells. Cell Rep. 2013;3:1553-66 pubmed publisher
chromatin immunoprecipitation; human	Kharbanda A, Rajabi H, Jin C, Raina D, Kufe D. Oncogenic MUC1-C promotes tamoxifen resistance in human breast cancer. Mol Cancer Res. 2013;11:714-23 pubmed publisher
immunohistochemistry; chicken	Katayama M, Fukuda T, Narabara K, Abe A, Kondo Y. Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis. Biosci Biotechnol Biochem. 2012;76:2003-7 pubmed
chromatin immunoprecipitation; human; 1:35	Privette Vinnedge L, Ho S, Wikenheiser Brokamp K, Wells S. The DEK oncogene is a target of steroid hormone receptor signaling in breast cancer. PLoS ONE. 2012;7:e46985 pubmed publisher
immunohistochemistry; mouse; 1:100 western blot; mouse; 1:100	Kam M, Lee K, Tam P, Lui V. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. PLoS ONE. 2012;7:e35799 pubmed publisher
chromatin immunoprecipitation; human	He H, Meyer C, Chen M, Jordan V, Brown M, Liu X. Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics. Genome Res. 2012;22:1015-25 pubmed publisher
western blot; human	Tuttle R, Miller K, Maiorano J, Termuhlen P, Gao Y, Berberich S. Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence. Int J Cancer. 2012;130:2291-9 pubmed publisher
immunoprecipitation; mouse	Miranda Carboni G, Guemes M, Bailey S, Anaya E, Corselli M, Peault B, et al. GATA4 regulates estrogen receptor-alpha-mediated osteoblast transcription. Mol Endocrinol. 2011;25:1126-36 pubmed publisher
chromatin immunoprecipitation; mouse	Zhao L, Glazov E, Pattabiraman D, Al Owaidi F, Zhang P, Brown M, et al. Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res. 2011;39:4664-79 pubmed publisher
EMSA; human	Shu F, Sidell N, Yang D, Kallen C. The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses. J Steroid Biochem Mol Biol. 2010;120:172-9 pubmed publisher
chromatin immunoprecipitation; human	Badve S, Collins N, Bhat Nakshatri P, Turbin D, Leung S, Thorat M, et al. Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications. Am J Pathol. 2010;176:2139-49 pubmed publisher
chromatin immunoprecipitation; human EMSA; human	Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed publisher
immunocytochemistry; human; 1:100	Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 pubmed publisher
western blot; human	Flamini M, Sanchez A, Goglia L, Tosi V, Genazzani A, Simoncini T. Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells. Mol Hum Reprod. 2009;15:675-85 pubmed publisher
chromatin immunoprecipitation; mouse	Tallack M, Keys J, Humbert P, Perkins A. EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2. J Biol Chem. 2009;284:20966-74 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed publisher
chromatin immunoprecipitation; human	Lupien M, Eeckhoute J, Meyer C, Krum S, Rhodes D, Liu X, et al. Coactivator function defines the active estrogen receptor alpha cistrome. Mol Cell Biol. 2009;29:3413-23 pubmed publisher
chromatin immunoprecipitation; human EMSA; human	Wang C, Yu J, Kallen C. Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells. PLoS ONE. 2008;3:e3523 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Giretti M, Fu X, De Rosa G, Sarotto I, Baldacci C, Garibaldi S, et al. Extra-nuclear signalling of estrogen receptor to breast cancer cytoskeletal remodelling, migration and invasion. PLoS ONE. 2008;3:e2238 pubmed publisher
blocking or activating experiments; mouse immunocytochemistry; mouse; 1:50 western blot; mouse; 1:400	Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed publisher
immunocytochemistry; mouse	Krum S, Miranda Carboni G, Hauschka P, Carroll J, Lane T, Freedman L, et al. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival. EMBO J. 2008;27:535-45 pubmed publisher
chromatin immunoprecipitation; human	Caslini C, Yang Z, El Osta M, Milne T, Slany R, Hess J. Interaction of MLL amino terminal sequences with menin is required for transformation. Cancer Res. 2007;67:7275-83 pubmed
chromatin immunoprecipitation; human	Tallack M, Keys J, Perkins A. Erythroid Kruppel-like factor regulates the G1 cyclin dependent kinase inhibitor p18INK4c. J Mol Biol. 2007;369:313-21 pubmed
chromatin immunoprecipitation; rat; 2 ug western blot; rat; 1:300	Kazi A, Koos R. Estrogen-induced activation of hypoxia-inducible factor-1alpha, vascular endothelial growth factor expression, and edema in the uterus are mediated by the phosphatidylinositol 3-kinase/Akt pathway. Endocrinology. 2007;148:2363-74 pubmed
chromatin immunoprecipitation; human immunoprecipitation; human western blot; human	Hirano S, Furutama D, Hanafusa T. Physiologically high concentrations of 17beta-estradiol enhance NF-kappaB activity in human T cells. Am J Physiol Regul Integr Comp Physiol. 2007;292:R1465-71 pubmed
western blot; mouse; 1:2000 chromatin immunoprecipitation; human	Zhang Z, Teng C. Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression. Mol Cell Endocrinol. 2007;264:128-41 pubmed
chromatin immunoprecipitation; human	Carroll J, Meyer C, Song J, Li W, Geistlinger T, Eeckhoute J, et al. Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006;38:1289-97 pubmed
immunoprecipitation; rat; 1:250	Mannella P, Brinton R. Estrogen receptor protein interaction with phosphatidylinositol 3-kinase leads to activation of phosphorylated Akt and extracellular signal-regulated kinase 1/2 in the same population of cortical neurons: a unified mechanism of estrogen action. J Neurosci. 2006;26:9439-47 pubmed
immunocytochemistry; human western blot; human	Simoncini T, Scorticati C, Mannella P, Fadiel A, Giretti M, Fu X, et al. Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway. Mol Endocrinol. 2006;20:1756-71 pubmed
immunoprecipitation; human; 1:30 western blot; human	Cheng A, Jin V, Fan M, Smith L, Liyanarachchi S, Yan P, et al. Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to estrogen receptor-alpha responsive promoters. Mol Cell. 2006;21:393-404 pubmed
immunohistochemistry; rat; 1:150	Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, et al. Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat. Biochem Biophys Res Commun. 2006;341:755-62 pubmed
chromatin immunoprecipitation; mouse	Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
chromatin immunoprecipitation; human	Jeffy B, Hockings J, Kemp M, Morgan S, Hager J, Beliakoff J, et al. An estrogen receptor-alpha/p300 complex activates the BRCA-1 promoter at an AP-1 site that binds Jun/Fos transcription factors: repressive effects of p53 on BRCA-1 transcription. Neoplasia. 2005;7:873-82 pubmed
immunoprecipitation; human	Wessler S, Otto C, Wilck N, Stangl V, Fritzemeier K. Identification of estrogen receptor ligands leading to activation of non-genomic signaling pathways while exhibiting only weak transcriptional activity. J Steroid Biochem Mol Biol. 2006;98:25-35 pubmed
chromatin immunoprecipitation; human	Carroll J, Liu X, Brodsky A, Li W, Meyer C, Szary A, et al. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005;122:33-43 pubmed
immunoprecipitation; human	Sentis S, Le Romancer M, Bianchin C, Rostan M, Corbo L. Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity. Mol Endocrinol. 2005;19:2671-84 pubmed
chromatin immunoprecipitation; human immunoprecipitation; human western blot; human	Park K, Krishnan V, O Malley B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
	Mei C, Xin L, Liu Y, Lin J, Xian H, Zhang X, et al. Establishment of a New Cell Line of Canine Mammary Tumor CMT-1026. Front Vet Sci. 2021;8:744032 pubmed publisher
	Jin F, Li J, Zhang Y, Liu X, Cai M, Liu M, et al. A functional motif of long noncoding RNA Nron against osteoporosis. Nat Commun. 2021;12:3319 pubmed publisher
	Brodzki A, Łopuszyński W, Millan Y, Tatara M, Brodzki P, Kulpa K, et al. Androgen and Estrogen Receptor Expression in Different Types of Perianal Gland Tumors in Male Dogs. Animals (Basel). 2021;11: pubmed publisher
	Stokes K, Alston Mills B, Teng C. Estrogen response element and the promoter context of the human and mouse lactoferrin genes influence estrogen receptor alpha-mediated transactivation activity in mammary gland cells. J Mol Endocrinol. 2004;33:315-34 pubmed
	Capiati D, Rossi A, Picotto G, Benassati S, Boland R. Inhibition of serum-stimulated mitogen activated protein kinase by 1alpha,25(OH)2-vitamin D3 in MCF-7 breast cancer cells. J Cell Biochem. 2004;93:384-97 pubmed
	Kirma N, Luthra R, Jones J, Liu Y, Nair H, Mandava U, et al. Overexpression of the colony-stimulating factor (CSF-1) and/or its receptor c-fms in mammary glands of transgenic mice results in hyperplasia and tumor formation. Cancer Res. 2004;64:4162-70 pubmed
	Flötotto T, Niederacher D, Hohmann D, Heimerzheim T, Dall P, Djahansouzi S, et al. Molecular mechanism of estrogen receptor (ER)alpha-specific, estradiol-dependent expression of the progesterone receptor (PR) B-isoform. J Steroid Biochem Mol Biol. 2004;88:131-42 pubmed
	Letting D, Chen Y, Rakowski C, Reedy S, Blobel G. Context-dependent regulation of GATA-1 by friend of GATA-1. Proc Natl Acad Sci U S A. 2004;101:476-81 pubmed
	Wu K, Tobias M, Kelley D. Estrogen receptor expression in laryngeal muscle in relation to estrogen-dependent increases in synapse strength. Neuroendocrinology. 2003;78:72-80 pubmed
	Simoncini T, Rabkin E, Liao J. Molecular basis of cell membrane estrogen receptor interaction with phosphatidylinositol 3-kinase in endothelial cells. Arterioscler Thromb Vasc Biol. 2003;23:198-203 pubmed
	DiRenzo J, Signoretti S, Nakamura N, Rivera Gonzalez R, Sellers W, Loda M, et al. Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. Cancer Res. 2002;62:89-98 pubmed
	González Agüero G, Ondarza R, Gamboa Dominguez A, Cerbon M, Camacho Arroyo I. Progesterone receptor isoforms expression pattern in human astrocytomas. Brain Res Bull. 2001;56:43-8 pubmed
	Schlegel A, Wang C, Pestell R, Lisanti M. Ligand-independent activation of oestrogen receptor alpha by caveolin-1. Biochem J. 2001;359:203-10 pubmed
	Monje P, Zanello S, Holick M, Boland R. Differential cellular localization of estrogen receptor alpha in uterine and mammary cells. Mol Cell Endocrinol. 2001;181:117-29 pubmed
	Biswas D, Cruz A, Pettit N, Mutter G, Pardee A. A therapeutic target for hormone-independent estrogen receptor-positive breast cancers. Mol Med. 2001;7:59-67 pubmed
	Kirma N, Gill K, Mandava U, Tekmal R. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice. Cancer Res. 2001;61:1910-8 pubmed
	Klinge C. Role of estrogen receptor ligand and estrogen response element sequence on interaction with chicken ovalbumin upstream promoter transcription factor (COUP-TF). J Steroid Biochem Mol Biol. 1999;71:1-19 pubmed
	Biswas D, Reddy P, Pickard M, Makkad B, Pettit N, Pardee A. Calmodulin is essential for estrogen receptor interaction with its motif and activation of responsive promoter. J Biol Chem. 1998;273:33817-24 pubmed
	Zwijsen R, Buckle R, Hijmans E, Loomans C, Bernards R. Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998;12:3488-98 pubmed
	Biswas D, Averboukh L, Sheng S, Martin K, Ewaniuk D, Jawde T, et al. Classification of breast cancer cells on the basis of a functional assay for estrogen receptor. Mol Med. 1998;4:454-67 pubmed
	Neuman E, Ladha M, Lin N, Upton T, Miller S, DiRenzo J, et al. Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4. Mol Cell Biol. 1997;17:5338-47 pubmed

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product information

Product Type :

Antibody

Product Name :

Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11)

Catalog # :

MA1-12692

Quantity :

100 µg

Price :

US 662

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat

Applications :

ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunoprecipitation: 2 µg/mL protein lysate, Western Blot: 1-2 µg/mL

Species :

Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat

Clone :

TE111.5D11

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Immunogen :

C-terminal fragment (aa 302-595) of human ER expressed in E coli.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: Assay-dependent, Gel Shift: Assay-dependent, Immunoprecipitation: 2 µg/mL protein lysate, Western Blot: 1-2 µg/mL

Aliases :

DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product

more info or order :

Invitrogen product webpage