This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Progesterone Receptor Antibody Cocktail
catalog :
MA1-12626
quantity :
100 µg
price :
US 546.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
hPRa2, hPRa3
reactivity :
domestic goat, African green monkey, deer, human, mouse, dogs, chicken, bovine, cat, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 71
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; domestic goat; 1:70; fig 6
Ogorevc J, Dovc P. Expression of estrogen receptor 1 and progesterone receptor in primary goat mammary epithelial cells. Anim Sci J. 2016;87:1464-1471 pubmed publisher
  • immunohistochemistry - paraffin section; chicken; 4 ug/ml; fig 2
González Morán M. Immunohistochemical localization of progesterone receptor isoforms and estrogen receptor alpha in the chicken oviduct magnum during development. Acta Histochem. 2015;117:681-7 pubmed publisher
  • immunohistochemistry; bovine; 1:20
de Sant Ana F, Carvalho F, de O Gamba C, Cassali G, Riet Correa F, Schild A. Mammary diffuse fibroadenomatoid hyperplasia in water buffalo (Bubalus bubalis): three cases. J Vet Diagn Invest. 2014;26:453-456 pubmed
  • chromatin immunoprecipitation; human
  • immunoprecipitation; human
  • western blot; human
Dressing G, Knutson T, Schiewer M, Daniel A, Hagan C, Diep C, et al. Progesterone receptor-cyclin D1 complexes induce cell cycle-dependent transcriptional programs in breast cancer cells. Mol Endocrinol. 2014;28:442-57 pubmed publisher
  • western blot; bovine; 1:250
O Shea L, Hensey C, Fair T. Progesterone regulation of AVEN protects bovine oocytes from apoptosis during meiotic maturation. Biol Reprod. 2013;89:146 pubmed publisher
  • chromatin immunoprecipitation; human; fig 7
  • western blot; human; fig 3
Hagan C, Knutson T, Lange C. A Common Docking Domain in Progesterone Receptor-B links DUSP6 and CK2 signaling to proliferative transcriptional programs in breast cancer cells. Nucleic Acids Res. 2013;41:8926-42 pubmed publisher
  • western blot; mouse
Bascom J, Radisky D, Koh E, Fata J, Lo A, Mori H, et al. Epimorphin is a novel regulator of the progesterone receptor isoform-a. Cancer Res. 2013;73:5719-29 pubmed publisher
  • immunohistochemistry; bovine
Pezzolato M, Richelmi G, Maurella C, Pitardi D, Varello K, Caramelli M, et al. Histopathology as a simple and reliable method to detect 17?-oestradiol illegal treatment in male calves. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30:1096-9 pubmed publisher
  • immunohistochemistry; bovine; 1:50
Palm F, Walter I, Nowotny N, Budik S, Helmreich M, Aurich C. Progestin treatment does not affect expression of cytokines, steroid receptors, oxytocin receptor, and cyclooxygenase 2 in fetal membranes and endometrium from pony mares at parturition. Theriogenology. 2013;79:59-68 pubmed publisher
  • immunocytochemistry; human
D Amato N, OSTRANDER J, Bowie M, Sistrunk C, Borowsky A, Cardiff R, et al. Evidence for phenotypic plasticity in aggressive triple-negative breast cancer: human biology is recapitulated by a novel model system. PLoS ONE. 2012;7:e45684 pubmed publisher
  • immunohistochemistry; dogs; 1:20
Gamba C, Dias E, Ribeiro L, Campos L, Estrela Lima A, Ferreira E, et al. Histopathological and immunohistochemical assessment of invasive micropapillary mammary carcinoma in dogs: a retrospective study. Vet J. 2013;196:241-6 pubmed publisher
  • immunocytochemistry; chicken; 2 ug/ml
  • immunohistochemistry; chicken
Machado S, Bahr J, Hales D, Braundmeier A, Quade B, Nowak R. Validation of the aging hen (Gallus gallus domesticus) as an animal model for uterine leiomyomas. Biol Reprod. 2012;87:86 pubmed publisher
  • immunohistochemistry; rhesus macaque
Almeida Francia C, Keator C, Mah K, Holden L, Hergert C, Slayden O. Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque. Hum Reprod. 2012;27:1723-34 pubmed publisher
  • immunohistochemistry; human
Schillaci R, Guzman P, Cayrol F, Beguelin W, Díaz Flaqué M, Proietti C, et al. Clinical relevance of ErbB-2/HER2 nuclear expression in breast cancer. BMC Cancer. 2012;12:74 pubmed publisher
  • immunohistochemistry; human
van der Horst P, Wang Y, Vandenput I, Kühne L, Ewing P, van Ijcken W, et al. Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer. PLoS ONE. 2012;7:e30840 pubmed publisher
  • western blot; human
Charles N, Thomas P, Lange C. Expression of membrane progesterone receptors (mPR/PAQR) in ovarian cancer cells: implications for progesterone-induced signaling events. Horm Cancer. 2010;1:167-76 pubmed publisher
  • immunohistochemistry; dogs; 1:20
Ferreira E, Gobbi H, Saraiva B, Cassali G. Histological and immunohistochemical identification of atypical ductal mammary hyperplasia as a preneoplastic marker in dogs. Vet Pathol. 2012;49:322-9 pubmed publisher
  • western blot; human
Kyo S, Sakaguchi J, Kiyono T, Shimizu Y, Maida Y, Mizumoto Y, et al. Forkhead transcription factor FOXO1 is a direct target of progestin to inhibit endometrial epithelial cell growth. Clin Cancer Res. 2011;17:525-37 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human; 1:200
  • western blot; human
Daniel A, Gaviglio A, Czaplicki L, Hillard C, Housa D, Lange C. The progesterone receptor hinge region regulates the kinetics of transcriptional responses through acetylation, phosphorylation, and nuclear retention. Mol Endocrinol. 2010;24:2126-38 pubmed publisher
  • immunohistochemistry; bovine; 1:200
Saruhan B, Sagsoz H, Akbalik M, Ketani M. Distribution of estrogen receptor ? and progesterone receptor B in the bovine oviduct during the follicular and luteal phases of the sexual cycle: an immunohistochemical and semi-quantitative study. Biotech Histochem. 2011;86:315-25 pubmed publisher
  • immunohistochemistry; bovine; 1:200
Sagsoz H, Akbalik M, Saruhan B, Ketani M. Localization of estrogen receptor ? and progesterone receptor B in bovine cervix and vagina during the follicular and luteal phases of the sexual cycle. Biotech Histochem. 2011;86:262-71 pubmed publisher
  • immunohistochemistry; mouse; 1:20
Ferreira E, Gobbi H, Saraiva B, Cassali G. Columnar cell lesions of the canine mammary gland: pathological features and immunophenotypic analysis. BMC Cancer. 2010;10:61 pubmed publisher
  • western blot; human
Afhüppe W, Beekman J, Otto C, Korr D, Hoffmann J, Fuhrmann U, et al. In vitro characterization of ZK 230211--A type III progesterone receptor antagonist with enhanced antiproliferative properties. J Steroid Biochem Mol Biol. 2010;119:45-55 pubmed publisher
  • western blot; human; 1:100
Chang C, Chiou P, Chen W, Li Y, Yiu J, Cheng Y, et al. Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines. Res Vet Sci. 2010;88:285-93 pubmed publisher
  • immunohistochemistry; human; 1:50
Wang Y, Hanifi Moghaddam P, Hanekamp E, Kloosterboer H, Franken P, Veldscholte J, et al. Progesterone inhibition of Wnt/beta-catenin signaling in normal endometrium and endometrial cancer. Clin Cancer Res. 2009;15:5784-93 pubmed publisher
  • immunohistochemistry; cat
Misirlioglu D, Nak D, Ozyigit M, Nak Y, Akkoc A. HER-2/neu (c-erbB-2) oncoprotein in hyperplastic endometrial polyps detected in two cats. J Feline Med Surg. 2009;11:885-8 pubmed publisher
  • immunohistochemistry; human; 1:50
Chakravarty D, Gupta N, Goda J, Srinivasan R, Patel F, Dhaliwal L. Steroid receptors, HER2/neu and Ki-67, in endometrioid type of endometrial carcinoma: Correlation with conventional histomorphological features of prognosis. Acta Histochem. 2010;112:355-63 pubmed publisher
  • immunohistochemistry; deer
Yanagawa Y, Matsuura Y, Suzuki M, Katagiri S, Tsubota T. Immunohistochemical localization of the estrogen receptor alpha (ERalpha) and progesterone receptor (PR) in the uterus of sika deer (Cervus nippon) during pregnancy. Jpn J Vet Res. 2008;56:139-49 pubmed
  • immunohistochemistry; human; 1:50
Chakravarty D, Srinivasan R, Ghosh S, Rajwanshi A, Gopalan S. Estrogen receptor beta (ERbeta) in endometrial simple hyperplasia and endometrioid carcinoma. Appl Immunohistochem Mol Morphol. 2008;16:535-42 pubmed publisher
  • immunohistochemistry; mouse; 1:50
Giulianelli S, Cerliani J, Lamb C, Fabris V, Bottino M, Gorostiaga M, et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int J Cancer. 2008;123:2518-31 pubmed publisher
  • western blot; human
Honorat M, Mesnier A, Di Pietro A, Lin V, Cohen P, Dumontet C, et al. Dexamethasone down-regulates ABCG2 expression levels in breast cancer cells. Biochem Biophys Res Commun. 2008;375:308-14 pubmed publisher
  • immunocytochemistry; African green monkey; 0.1 ug/ml
  • immunocytochemistry; human; 0.1 ug/ml
Bishop C, Filtz T, Zhang Y, Slayden O, Stormshak F. Progesterone suppresses an oxytocin-stimulated signal pathway in COS-7 cells transfected with the oxytocin receptor. Steroids. 2008;73:1367-74 pubmed publisher
  • immunohistochemistry; human; 4 ug/ml
Bao H, Yu D, Wang J, Qiu T, Yang J, Wang L. Predictive value of serum anti-p53 antibodies, carcino-embryonic antigen, carbohydrate antigen 15-3, estrogen receptor, progesterone receptor and human epidermal growth factor receptor-2 in taxane-based and anthracycline-based neoadjuvant chemotherap. Anticancer Drugs. 2008;19:317-23 pubmed
  • immunohistochemistry; human; 1:50
Peluso J, Liu X, Saunders M, Claffey K, Phoenix K. Regulation of ovarian cancer cell viability and sensitivity to cisplatin by progesterone receptor membrane component-1. J Clin Endocrinol Metab. 2008;93:1592-9 pubmed publisher
  • immunohistochemistry; bovine
Martin I, Torres Neto R, Oba E, Buratini J, Binelli M, Laufer Amorim R, et al. Immunohistochemical detection of receptors for oestrogen and progesterone in endometrial glands and stroma during the oestrous cycle in Nelore (Bos taurus indicus) cows. Reprod Domest Anim. 2008;43:415-21 pubmed publisher
  • immunohistochemistry; human; 1:400
Yaren A, Kelten C, Akbulut M, Teke Z, Duzcan E, Erdem E. Primary neuroendocrine carcinoma of the breast: a case report. Tumori. 2007;93:496-8 pubmed
  • immunocytochemistry; human
Brayman M, Dharmaraj N, Lagow E, Carson D. MUC1 expression is repressed by protein inhibitor of activated signal transducer and activator of transcription-y. Mol Endocrinol. 2007;21:2725-37 pubmed
  • immunocytochemistry; human
  • western blot; human
Zheng Z, Zheng S, Bay B, Aw S, C L Lin V. Anti-estrogenic mechanism of unliganded progesterone receptor isoform B in breast cancer cells. Breast Cancer Res Treat. 2008;110:111-25 pubmed
  • EMSA; human
  • western blot; human
Daniel A, Qiu M, Faivre E, Ostrander J, Skildum A, Lange C. Linkage of progestin and epidermal growth factor signaling: phosphorylation of progesterone receptors mediates transcriptional hypersensitivity and increased ligand-independent breast cancer cell growth. Steroids. 2007;72:188-201 pubmed
  • western blot; human
Hanifi Moghaddam P, Sijmons B, Ott M, van Ijcken W, Nowzari D, Kuhne E, et al. The hormone replacement therapy drug tibolone acts very similar to medroxyprogesterone acetate in an estrogen-and progesterone-responsive endometrial cancer cell line. J Mol Endocrinol. 2006;37:405-13 pubmed
  • immunohistochemistry; human; 1:200
Ishizuka M, Hatori M, Dohi O, Suzuki T, Miki Y, Tazawa C, et al. Expression profiles of sex steroid receptors in desmoid tumors. Tohoku J Exp Med. 2006;210:189-98 pubmed
  • western blot; human
Faivre E, Lange C. Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells. Mol Cell Biol. 2007;27:466-80 pubmed
  • immunohistochemistry; human; 1:100
Saito S, Ito K, Nagase S, Suzuki T, Akahira J, Okamura K, et al. Progesterone receptor isoforms as a prognostic marker in human endometrial carcinoma. Cancer Sci. 2006;97:1308-14 pubmed
  • immunocytochemistry; human; 1:50
Engmann L, Losel R, Wehling M, Peluso J. Progesterone regulation of human granulosa/luteal cell viability by an RU486-independent mechanism. J Clin Endocrinol Metab. 2006;91:4962-8 pubmed
  • immunohistochemistry; human; 4 ug/ml
Gao R, Bao H, Yang Q, Cong Q, Song J, Wang L. The presence of serum anti-p53 antibodies from patients with invasive ductal carcinoma of breast: correlation to other clinical and biological parameters. Breast Cancer Res Treat. 2005;93:111-5 pubmed
  • immunohistochemistry; human; 1:50
Cabioglu N, Sahin A, Doucet M, Yavuz E, Igci A, O Yildirim E, et al. Chemokine receptor CXCR4 expression in breast cancer as a potential predictive marker of isolated tumor cells in bone marrow. Clin Exp Metastasis. 2005;22:39-46 pubmed
  • immunohistochemistry; human; 1:100
Akhan S, Yavuz E, Tecer A, Iyibozkurt C, Topuz S, Tuzlali S, et al. The expression of Ki-67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study. Gynecol Oncol. 2005;99:36-42 pubmed
  • western blot; human
Leo J, Wang S, Guo C, Aw S, Zhao Y, Li J, et al. Gene regulation profile reveals consistent anticancer properties of progesterone in hormone-independent breast cancer cells transfected with progesterone receptor. Int J Cancer. 2005;117:561-8 pubmed
  • western blot; human; 1:200
Mrusek S, Classen Linke I, Vloet A, Beier H, Krusche C. Estradiol and medroxyprogesterone acetate regulated genes in T47D breast cancer cells. Mol Cell Endocrinol. 2005;235:39-50 pubmed
  • immunohistochemistry; human
Hasegawa J, Yanaihara A, Iwasaki S, Otsuka Y, Negishi M, Akahane T, et al. Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality. Hum Reprod. 2005;20:2194-200 pubmed
  • western blot; human
Fritah A, Saucier C, Mester J, Redeuilh G, Sabbah M. p21WAF1/CIP1 selectively controls the transcriptional activity of estrogen receptor alpha. Mol Cell Biol. 2005;25:2419-30 pubmed
  • immunohistochemistry; human; 1:100
Nishimura Y, Watanabe J, Jobo T, Hattori M, Arai T, Kuramoto H. Cytologic scoring of endometrioid adenocarcinoma of the endometrium. Cancer. 2005;105:8-12 pubmed
  • immunoprecipitation; human; 5 ug/time
  • immunocytochemistry; human; 1:500
  • immunohistochemistry; human; 1:25
  • western blot; human
Leslie K, Stein M, Kumar N, Dai D, Stephens J, Wandinger Ness A, et al. Progesterone receptor isoform identification and subcellular localization in endometrial cancer. Gynecol Oncol. 2005;96:32-41 pubmed
  • western blot; human
Skildum A, Faivre E, Lange C. Progesterone receptors induce cell cycle progression via activation of mitogen-activated protein kinases. Mol Endocrinol. 2005;19:327-39 pubmed
Segabinazzi L, Canisso I, Podico G, Cunha L, Novello G, Rosser M, et al. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel). 2021;10: pubmed publisher
El Sheikh Ali H, Scoggin K, Ruby R, Loynachan A, Boakari Y, Fernandes C, et al. Equine cervical remodeling during placentitis and the prepartum period: a transcriptomic approach. Reproduction. 2021;161:603-621 pubmed publisher
El Sheikh Ali H, Legacki E, Scoggin K, Loux S, Dini P, Esteller Vico A, et al. Steroid synthesis and metabolism in the equine placenta during placentitis. Reproduction. 2020;159:289-302 pubmed publisher
Ando H, Miyamoto T, Kashima H, Higuchi S, Ida K, Mvunta D, et al. Panobinostat Enhances Growth Suppressive Effects of Progestin on Endometrial Carcinoma by Increasing Progesterone Receptor and Mitogen-Inducible Gene-6. Horm Cancer. 2017;8:257-267 pubmed publisher
Brenner R, Slayden O. Steroid receptors in blood vessels of the rhesus macaque endometrium: a review. Arch Histol Cytol. 2004;67:411-6 pubmed
Greenberg L, Slayden O. Human endometriotic xenografts in immunodeficient RAG-2/gamma(c)KO mice. Am J Obstet Gynecol. 2004;190:1788-95; discussion 1795-6 pubmed
Guler G, Uner A, Guler N, Han S, Iliopoulos D, Hauck W, et al. The fragile genes FHIT and WWOX are inactivated coordinately in invasive breast carcinoma. Cancer. 2004;100:1605-14 pubmed
Roberts K, Bhatia K, Stanton P, Lord R. Proteomic analysis of selected prognostic factors of breast cancer. Proteomics. 2004;4:784-92 pubmed
Abd Elaziz M, Akahira J, Moriya T, Suzuki T, Yaegashi N, Sasano H. Nuclear receptor DAX-1 in human common epithelial ovarian carcinoma: an independent prognostic factor of clinical outcome. Cancer Sci. 2003;94:980-5 pubmed
Hanekamp E, Gielen S, Smid Koopman E, Kuhne L, De Ruiter P, Chadha Ajwani S, et al. Consequences of loss of progesterone receptor expression in development of invasive endometrial cancer. Clin Cancer Res. 2003;9:4190-9 pubmed
González Morán G, Camacho Arroyo I. Changes in the presence of progesterone receptor isoforms in the oviduct magnum of newly-hatched chicks after gonadotropins treatment. Life Sci. 2003;73:871-82 pubmed
Akahira J, Suzuki T, Ito K, Kaneko C, Darnel A, Moriya T, et al. Differential expression of progesterone receptor isoforms A and B in the normal ovary, and in benign, borderline, and malignant ovarian tumors. Jpn J Cancer Res. 2002;93:807-15 pubmed
Joki Erkkilä M, Karikoski R, Rantala I, Lenko H, Visakorpi T, Heinonen P. Gonadoblastoma and dysgerminoma associated with XY gonadal dysgenesis in an adolescent with chronic renal failure: a case of Frasier syndrome. J Pediatr Adolesc Gynecol. 2002;15:145-9 pubmed
González Morán G, García Izquierdo J, Camacho Arroyo I. Progesterone receptor isoforms in ovary of newly-hatched chick after gonadotropin treatment during embryonic development. Comp Biochem Physiol A Mol Integr Physiol. 2002;132:519-26 pubmed
Skinner D, Caraty A, Allingham R. Unmasking the progesterone receptor in the preoptic area and hypothalamus of the ewe: no colocalization with gonadotropin-releasing neurons. Endocrinology. 2001;142:573-9 pubmed
Akahira J, Inoue T, Suzuki T, Ito K, Konno R, Sato S, et al. Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies. Br J Cancer. 2000;83:1488-94 pubmed
O Brien S, Welter B, Mantzke K, Price T. Identification of progesterone receptor in human subcutaneous adipose tissue. J Clin Endocrinol Metab. 1998;83:509-13 pubmed
product information
Product Type :
Antibody
Product Name :
Progesterone Receptor Antibody Cocktail
Catalog # :
MA1-12626
Quantity :
100 µg
Price :
US 546.00
Clonality :
Cocktail
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
ChIP Assay: 2 µL/10^6 cells, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:25-1:50, Immunohistochemistry (Paraffin): 1:25-1:50, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL
Species :
Human
Clone :
hPRa2, hPRa3
Isotype :
IgG
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
Immunogen :
Human endometrial carcinoma from in athymic mice.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 2 µL/10^6 cells, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 1:25-1:50, Immunohistochemistry (Paraffin): 1:25-1:50, Immunoprecipitation: 2 µg/mL, Western Blot: 1 µg/mL
Aliases :
9930019P03Rik; BB114106; ENSMUSG00000074510; LOW QUALITY PROTEIN: progesterone receptor; NR3C3; Nuclear receptor subfamily 3 group C member 3; p4 receptor; PGR; pot. alt. progesterone receptor; putative; PR; PR-A; PR-B; Progesterone receptor; progesterone receptor A; progesterone-receptor; putative form B; steroid hormone receptor; testicular progesterone receptor; unnamed protein product
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA