Estrogen Receptor alpha Monoclonal Antibody (AER311) | Invitrogen MA1-12600 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Estrogen Receptor alpha Monoclonal Antibody (AER311)

catalog :

MA1-12600

quantity :

100 µg

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AER311

reactivity :

human, mouse, bovine, domestic horse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; bovine; 1:200	Rantala M, Mutikainen M, Schuler G, Katila T, Taponen J. Endometrial expression of progesterone, estrogen, and oxytocin receptors and of 20?-hydroxysteroid dehydrogenase and cyclooxygenase II 2 and 5 days after ovulation in induced short and normal estrous cycles in dairy cows. Theriogenology. 2014;81:1181-8 pubmed publisher
western blot; human; 1:100	Choudhuri R, DeGraff W, Gamson J, Mitchell J, Cook J. Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines. Front Oncol. 2011;1:19 pubmed publisher
immunocytochemistry; human	Larson J, Goodman L, Tan Y, DeFazio Eli L, Paquet A, Cook J, et al. Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens. Patholog Res Int. 2010;2010:814176 pubmed publisher
immunohistochemistry; human	Peng X, Li T, Xia E, Xia C, Liu Y, Yu D. A comparison of oestrogen receptor and progesterone receptor expression in endometrial polyps and endometrium of premenopausal women. J Obstet Gynaecol. 2009;29:340-6 pubmed
immunohistochemistry; domestic horse	Abd Elnaeim M, Derar I, Wilsher S, Allen W, Leiser R, Schuler G. Immunohistochemical localization of oestrogen receptors alpha and beta, progesterone receptor and aromatase in the equine placenta. Reprod Domest Anim. 2009;44:312-9 pubmed publisher
western blot; human; 1:100	Setlur S, Mertz K, Hoshida Y, Demichelis F, Lupien M, Perner S, et al. Estrogen-dependent signaling in a molecularly distinct subclass of aggressive prostate cancer. J Natl Cancer Inst. 2008;100:815-25 pubmed publisher
western blot; mouse	Tekmal R, Nair H, Perla R, Kirma N. HER-2/neu x aromatase double transgenic mice model: the effects of aromatase overexpression on mammary tumorigenesis. J Steroid Biochem Mol Biol. 2007;106:111-8 pubmed
western blot; mouse	Min C, Kirsch K, Zhao Y, Jeay S, Palamakumbura A, Trackman P, et al. The tumor suppressor activity of the lysyl oxidase propeptide reverses the invasive phenotype of Her-2/neu-driven breast cancer. Cancer Res. 2007;67:1105-12 pubmed
western blot; human	York B, Lou D, Noonan D. Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus. Mol Cancer Res. 2006;4:885-97 pubmed
immunohistochemistry; mouse; 1:100	Mehra R, Sharma K, Nyakas C, Vij U. Estrogen receptor alpha and beta immunoreactive neurons in normal adult and aged female rat hippocampus: a qualitative and quantitative study. Brain Res. 2005;1056:22-35 pubmed
western blot; human	Bourguignon L, Gilad E, Rothman K, Peyrollier K. Hyaluronan-CD44 interaction with IQGAP1 promotes Cdc42 and ERK signaling, leading to actin binding, Elk-1/estrogen receptor transcriptional activation, and ovarian cancer progression. J Biol Chem. 2005;280:11961-72 pubmed
	Tanmahasamut P, Liu J, Hendry L, Sidell N. Conjugated linoleic acid blocks estrogen signaling in human breast cancer cells. J Nutr. 2004;134:674-80 pubmed
	Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, et al. Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition. Biol Reprod. 2002;66:976-82 pubmed
	Feng W, Graumann K, Hahn R, Jungbauer A. Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography. J Chromatogr A. 1999;852:161-73 pubmed
	Huang A, Leygue E, Snell L, Murphy L, Watson P. Expression of estrogen receptor variant messenger RNAs and determination of estrogen receptor status in human breast cancer. Am J Pathol. 1997;150:1827-33 pubmed

product information

Product Type :

Antibody

Product Name :

Estrogen Receptor alpha Monoclonal Antibody (AER311)

Catalog # :

MA1-12600

Quantity :

100 µg

Price :

US 496.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Bovine, Human, Mouse

Applications :

ChIP Assay: 2.5 µg/10^6 cells, ELISA: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Bovine, Human, Mouse

Clone :

AER311

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Immunogen :

Estrogen receptors from calf uterus.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 2.5 µg/10^6 cells, ELISA: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 group A member 1; RNESTROR; RP1-130E4.1; unnamed protein product

more info or order :

Invitrogen product webpage