This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cytokeratin 18 Monoclonal Antibody (DC10)
catalog :
MA1-12589
quantity :
100µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DC10
reactivity :
African green monkey, human, mouse, Rhesus monkey
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 42
Published Application/Species/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 1b
  • immunocytochemistry (knockdown validation); human; fig 2f
  • western blot; human; fig 2e
Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, et al. Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Sci Rep. 2016;6:34472 pubmed publisher
  • western blot; human; 1:1000; fig 2c, ED4b
  • immunocytochemistry; human; 1:2000; fig ED1g
Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;: pubmed publisher
  • western blot; human; fig 5
Thakkar A, Wang B, Picon-Ruiz M, Buchwald P, Ince T. Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer. Breast Cancer Res Treat. 2016;157:77-90 pubmed publisher
  • immunocytochemistry; human; fig 3g
  • western blot; human; fig 3h
Kuga T, Sasaki M, Mikami T, Miake Y, Adachi J, Shimizu M, et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Sci Rep. 2016;6:26557 pubmed publisher
  • immunocytochemistry; human; fig s10
Hines W, Yaswen P, Bissell M. Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias. Nat Commun. 2015;6:6927 pubmed publisher
  • immunohistochemistry; human; 1:100
Basak K, Kiroğlu K. Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report. Medicine (Baltimore). 2014;93:e246 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Zollner G, Thueringer A, Lackner C, Fickert P, Trauner M. Alterations of canalicular ATP-binding cassette transporter expression in drug-induced liver injury. Digestion. 2014;90:81-8 pubmed publisher
  • immunocytochemistry; human; 1:50
Imbalzano K, Cohet N, Wu Q, Underwood J, Imbalzano A, Nickerson J. Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections. PLoS ONE. 2013;8:e55628 pubmed publisher
  • western blot; human; 1:200; fig 6
Page J, Johnson M, Olsavsky K, Strom S, Zarbl H, Omiecinski C. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci. 2007;97:384-97 pubmed
  • flow cytometry; human; 1:200; fig s2
  • immunocytochemistry; human; 1:200; fig 6
  • western blot; human; 1:200; fig 5
Lian X, Selekman J, Bao X, Hsiao C, Zhu K, Palecek S. A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells. PLoS ONE. 2013;8:e60016 pubmed publisher
  • immunocytochemistry; human; fig 1
  • immunohistochemistry; human; fig 9
Duss S, André S, Nicoulaz A, Fiche M, Bonnefoi H, Brisken C, et al. An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells. Breast Cancer Res. 2007;9:R38 pubmed
Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
Snider N, Weerasinghe S, Iniguez-Lluhi J, Herrmann H, Omary M. Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation. J Biol Chem. 2011;286:2273-84 pubmed publisher
Gurbuz Y, Kose N, Aydin O, Ozturk M. An immunohistochemical profile of giant cell carcinoma of the larynx. Auris Nasus Larynx. 2007;34:413-6 pubmed
Ku N, Gish R, Wright T, Omary M. Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med. 2001;344:1580-7 pubmed
Røsland G, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009;69:5331-9 pubmed publisher
Chang C, Chiou P, Chen W, Li Y, Yiu J, Cheng Y, et al. Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines. Res Vet Sci. 2010;88:285-93 pubmed publisher
Tao G, Li D, Zhou Q, Toivola D, Strnad P, Sandesara N, et al. Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation. J Pathol. 2008;215:164-74 pubmed publisher
Németh I, Tiszlavicz L. Biphenotypic surface epithelial cells in the gastrointestinal tube with mixed epithelial-myofibroblastic differentiation: a paradigm. Pathol Oncol Res. 2012;18:391-6 pubmed publisher
Apaydin R, Gurbuz Y, Bayramgurler D, Muezzinoglu B, Bilen N. Cytokeratin expression in lichen amyloidosus and macular amyloidosis. J Eur Acad Dermatol Venereol. 2004;18:305-9 pubmed
Marian C, Boilă A, Soanca D, Malau M, Podeanu D, Resetkova E, et al. Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature. APMIS. 2013;121:272-9 pubmed publisher
Ku N, Toivola D, Strnad P, Omary M. Cytoskeletal keratin glycosylation protects epithelial tissue from injury. Nat Cell Biol. 2010;12:876-85 pubmed publisher
Singla A, Moons D, Snider N, Wagenmaker E, Jayasundera V, Omary M. Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria. Hepatology. 2012;56:322-31 pubmed publisher
Ku N, Toivola D, Zhou Q, Tao G, Zhong B, Omary M. Studying simple epithelial keratins in cells and tissues. Methods Cell Biol. 2004;78:489-517 pubmed
Tao G, Toivola D, Zhou Q, Strnad P, Xu B, Michie S, et al. Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner. J Cell Sci. 2006;119:1425-32 pubmed
Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer. J Cell Sci. 2013;126:4721-31 pubmed publisher
Toivola D, Ku N, Resurreccion E, Nelson D, Wright T, Omary M. Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease. Hepatology. 2004;40:459-66 pubmed
Kalantari F, Miao D, Emadali A, Tzimas G, Goltzman D, Vali H, et al. Cellular and molecular mechanisms of abnormal calcification following ischemia-reperfusion injury in human liver transplantation. Mod Pathol. 2007;20:357-66 pubmed
Liu Y, Liu H, Han B, Zhang J. Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis. Cancer Res. 2006;66:3248-55 pubmed
Metallo C, Ji L, de Pablo J, Palecek S. Directed differentiation of human embryonic stem cells to epidermal progenitors. Methods Mol Biol. 2010;585:83-92 pubmed publisher
Wood C, Kaplan J, Fontenot M, Williams J, Cline J. Endometrial profile of tamoxifen and low-dose estradiol combination therapy. Clin Cancer Res. 2010;16:946-56 pubmed publisher
Ku N, Soetikno R, Omary M. Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury. Hepatology. 2003;37:1006-14 pubmed
Yalcin-Ozuysal O, Fiche M, Guitierrez M, Wagner K, Raffoul W, Brisken C. Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates. Cell Death Differ. 2010;17:1600-12 pubmed publisher
Wood C, Hester J, Cline J. Mammary gland development in early pubertal female macaques. Toxicol Pathol. 2007;35:795-805 pubmed
Tao G, Strnad P, Zhou Q, Kamal A, Zhang L, Madani N, et al. Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease. Clin Gastroenterol Hepatol. 2007;5:857-64 pubmed
Yu G, Goodloe S, D'Angelis C, McGrath B, Chen F. Giant clear cell hidradenoma of the knee. J Cutan Pathol. 2010;37:e37-41 pubmed publisher
Robertson F, Ogasawara M, Ye Z, Chu K, Pickei R, Debeb B, et al. Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype. J Biomol Screen. 2010;15:820-9 pubmed publisher
Tao G, Nakamichi I, Ku N, Wang J, Frolkis M, Gong X, et al. Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res. 2006;312:411-22 pubmed
Szponar A, Kovacs G. Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas. Virchows Arch. 2012;460:423-7 pubmed publisher
Guo Y, Zi X, Koontz Z, Kim A, Xie J, Gorlick R, et al. Blocking Wnt/LRP5 signaling by a soluble receptor modulates the epithelial to mesenchymal transition and suppresses met and metalloproteinases in osteosarcoma Saos-2 cells. J Orthop Res. 2007;25:964-71 pubmed
Zi X, Guo Y, Simoneau A, Hope C, Xie J, Holcombe R, et al. Expression of Frzb/secreted Frizzled-related protein 3, a secreted Wnt antagonist, in human androgen-independent prostate cancer PC-3 cells suppresses tumor growth and cellular invasiveness. Cancer Res. 2005;65:9762-70 pubmed
Guo Y, Xie J, Rubin E, Tang Y, Lin F, Zi X, et al. Frzb, a secreted Wnt antagonist, decreases growth and invasiveness of fibrosarcoma cells associated with inhibition of Met signaling. Cancer Res. 2008;68:3350-60 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Cytokeratin 18 Monoclonal Antibody (DC10)
Catalog # :
MA1-12589
Quantity :
100µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Human
Applications :
Immunohistochemistry (Frozen): Assay Dependent, Immunohistochemistry (Paraffin): Assay Dependent, Western Blot: Assay Dependent
Species :
Human
Clone :
DC10
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Immunogen :
Human PMC-42 breat carcinoma Cells.
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Frozen): Assay Dependent, Immunohistochemistry (Paraffin): Assay Dependent, Western Blot: Assay Dependent
Aliases :
CYK18, K18
PubMed References :
11372009, 12717381, 15096140, 15368451, 15646629, 16266997, 16343483, 16540677, 16554440, 17318900, 17329237, 17334330, 17376621, 17509943, 17573968, 17943653, 18393369, 18451162, 19509230, 19615032, 19748110, 19907998, 20103679, 20379195, 20639504, 20729838, 21062750, 22382985, 23030630, 23358244, 23405182, 23527294, 23902688, 25196354, 25501093, 25896888, 27120467
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA