Phosphotyrosine Monoclonal Antibody (pY20), HRP | Invitrogen MA1-12445 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phosphotyrosine Monoclonal Antibody (pY20), HRP

catalog :

MA1-12445

quantity :

50 ug

price :

US 292.00

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

PY20

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

citations: 16

Reference
Malavaki C, Roussidis A, Gialeli C, Kletsas D, Tsegenidis T, Theocharis A, et al. Imatinib as a key inhibitor of the platelet-derived growth factor receptor mediated expression of cell surface heparan sulfate proteoglycans and functional properties of breast cancer cells. FEBS J. 2013;280:2477-89 pubmed publisher
Crockett J, Newman D, Newman P. PECAM-1 functions as a negative regulator of laminin-induced platelet activation. J Thromb Haemost. 2010;8:1584-93 pubmed publisher
Oda H, Fujimoto M, Patrick M, Chida D, Sato Y, Azuma Y, et al. RhoH plays critical roles in Fc epsilon RI-dependent signal transduction in mast cells. J Immunol. 2009;182:957-62 pubmed
Alvarez Errico D, Sayos J, Lopez Botet M. The IREM-1 (CD300f) inhibitory receptor associates with the p85alpha subunit of phosphoinositide 3-kinase. J Immunol. 2007;178:808-16 pubmed
Favier B, Alam A, Barron P, Bonnin J, Laboudie P, Fons P, et al. Neuropilin-2 interacts with VEGFR-2 and VEGFR-3 and promotes human endothelial cell survival and migration. Blood. 2006;108:1243-50 pubmed
Vadlamudi R, Manavathi B, Balasenthil S, Nair S, Yang Z, Sahin A, et al. Functional implications of altered subcellular localization of PELP1 in breast cancer cells. Cancer Res. 2005;65:7724-32 pubmed
Alam A, Herault J, Barron P, Favier B, Fons P, Delesque Touchard N, et al. Heterodimerization with vascular endothelial growth factor receptor-2 (VEGFR-2) is necessary for VEGFR-3 activity. Biochem Biophys Res Commun. 2004;324:909-15 pubmed
Matheson S, McNamee J, Wang T, Alaoui Jamali M, Tari A, Jean Claude B. The combi-targeting concept: dissection of the binary mechanism of action of the combi-triazene SMA41 in vitro and antitumor activity in vivo. J Pharmacol Exp Ther. 2004;311:1163-70 pubmed
Kenny T, Keen C, Jones P, Kung H, Schmitz H, Gershwin M. Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H2O2. Exp Biol Med (Maywood). 2004;229:765-71 pubmed
Thorne R, Pronk G, Padmanabhan V, Frey W. Delivery of insulin-like growth factor-I to the rat brain and spinal cord along olfactory and trigeminal pathways following intranasal administration. Neuroscience. 2004;127:481-96 pubmed
Burtrum D, Zhu Z, Lu D, Anderson D, Prewett M, Pereira D, et al. A fully human monoclonal antibody to the insulin-like growth factor I receptor blocks ligand-dependent signaling and inhibits human tumor growth in vivo. Cancer Res. 2003;63:8912-21 pubmed
Tamura I, Sakaki T, Chaqour B, Howard P, Ikeo T, Macarak E. Correlation of P-cadherin and beta-catenin expression and phosphorylation with carcinogenesis in rat tongue cancer induced with 4-nitroquinoline 1-oxide. Oral Oncol. 2003;39:506-14 pubmed
Brahimi F, Matheson S, Dudouit F, McNamee J, Tari A, Jean Claude B. Inhibition of epidermal growth factor receptor-mediated signaling by "Combi-triazene" BJ2000, a new probe for Combi-Targeting postulates. J Pharmacol Exp Ther. 2002;303:238-46 pubmed
Nilsen J, Chen S, Brinton R. Dual action of estrogen on glutamate-induced calcium signaling: mechanisms requiring interaction between estrogen receptors and src/mitogen activated protein kinase pathway. Brain Res. 2002;930:216-34 pubmed
Lukong K, Seyrantepe V, Landry K, Trudel S, Ahmad A, Gahl W, et al. Intracellular distribution of lysosomal sialidase is controlled by the internalization signal in its cytoplasmic tail. J Biol Chem. 2001;276:46172-81 pubmed
Sayos J, Nguyen K, Wu C, Stepp S, Howie D, Schatzle J, et al. Potential pathways for regulation of NK and T cell responses: differential X-linked lymphoproliferative syndrome gene product SAP interactions with SLAM and 2B4. Int Immunol. 2000;12:1749-57 pubmed

product information

Product Type :

Antibody

Product Name :

Phosphotyrosine Monoclonal Antibody (pY20), HRP

Catalog # :

MA1-12445

Quantity :

50 ug

Price :

US 292.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Chemical

Applications :

ELISA: 1:2,000, Immunohistochemistry: 1:500, Immunoprecipitation: 1:500, Western Blot: 1:2,000

Species :

Chemical

Clone :

pY20

Isotype :

IgG2b

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Immunogen :

Phosphotyrosine coupled to carrier protein.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:2,000, Immunohistochemistry: 1:500, Immunoprecipitation: 1:500, Western Blot: 1:2,000

Aliases :

Phosphotyrosine; pTyr; pY