This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HA Tag Monoclonal Antibody (12CA5)
catalog :
MA1-12429
quantity :
100 ug
price :
US 478.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12ca5
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 53
Reference
Houles T, Boucher J, Lavoie G, Macleod G, Lin S, Angers S, et al. The CDK12 inhibitor SR-4835 functions as a molecular glue that promotes cyclin K degradation in melanoma. Cell Death Discov. 2023;9:459 pubmed publisher
Kordus S, Kroh H, Rodr xed guez R, Shrem R, Peritore Galve F, Shupe J, et al. Nanobodies against C. difficile TcdA and TcdB reveal unexpected neutralizing epitopes and provide a toolkit for toxin quantitation in vivo. PLoS Pathog. 2023;19:e1011496 pubmed publisher
Spencer Z, Ng V, Benchabane H, Siddiqui G, Duwadi D, Maines B, et al. The USP46 deubiquitylase complex increases Wingless/Wnt signaling strength by stabilizing Arrow/LRP6. Nat Commun. 2023;14:6174 pubmed publisher
van de Kooij B, de Vries E, Rooswinkel R, Janssen G, Kok F, van Veelen P, et al. N-terminal acetylation can stabilize proteins independent of their ubiquitination. Sci Rep. 2023;13:5333 pubmed publisher
Rossitto M, D xe9 jardin S, Rands C, Le Gras S, Migale R, Rafiee M, et al. TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway. Nat Commun. 2022;13:4412 pubmed publisher
Mitgau J, Franke J, Schinner C, Stephan G, Berndt S, Placantonakis D, et al. The N Terminus of Adhesion G Protein-Coupled Receptor GPR126/ADGRG6 as Allosteric Force Integrator. Front Cell Dev Biol. 2022;10:873278 pubmed publisher
Baron D, Fenton A, Saez Atienzar S, Giampetruzzi A, Sreeram A, Shankaracharya -, et al. ALS-associated KIF5A mutations abolish autoinhibition resulting in a toxic gain of function. Cell Rep. 2022;39:110598 pubmed publisher
Garipler G, Lu C, Morrissey A, Lopez Zepeda L, Pei Y, Vidal S, et al. The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by repressing pro-differentiation genes. Cell Rep. 2022;38:110524 pubmed publisher
Hayashi H, Kishi T. A Set of Plasmid-Based Modules for Easy Switching of C-Terminal Epitope Tags in Saccharomyces cerevisiae. Microorganisms. 2021;9: pubmed publisher
Galati E, Bosio M, Novarina D, Chiara M, Bernini G, Mozzarelli A, et al. VID22 counteracts G-quadruplex-induced genome instability. Nucleic Acids Res. 2021;49:12785-12804 pubmed publisher
Gough R, Jones M, Zacharchenko T, Le S, Yu M, Jacquemet G, et al. Talin mechanosensitivity is modulated by a direct interaction with cyclin-dependent kinase-1. J Biol Chem. 2021;297:100837 pubmed publisher
Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold G, et al. Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. Sci Rep. 2021;11:5412 pubmed publisher
Choi H, Zou S, Wu J, Wang H, Phan L, Li K, et al. EGF Relays Signals to COP1 and Facilitates FOXO4 Degradation to Promote Tumorigenesis. Adv Sci (Weinh). 2020;7:2000681 pubmed publisher
Micic J, Li Y, Wu S, Wilson D, Tutuncuoglu B, Gao N, et al. Coupling of 5S RNP rotation with maturation of functional centers during large ribosomal subunit assembly. Nat Commun. 2020;11:3751 pubmed publisher
Hsieh Y, Makrantoni V, Robertson D, Marston A, Murray A. Evolutionary repair: Changes in multiple functional modules allow meiotic cohesin to support mitosis. PLoS Biol. 2020;18:e3000635 pubmed publisher
Zhao N, Kamijo K, Fox P, Oda H, Morisaki T, Sato Y, et al. A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Nat Commun. 2019;10:2947 pubmed publisher
Baeuerle P, Ding J, Patel E, Thorausch N, Horton H, Gierut J, et al. Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response. Nat Commun. 2019;10:2087 pubmed publisher
Walker M, Lindner S. Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii. J Biol Chem. 2019;294:9555-9566 pubmed publisher
van Domselaar R, Njenda D, Rao R, Sonnerborg A, Singh K, Neogi U. HIV-1 Subtype C with PYxE Insertion Has Enhanced Binding of Gag-p6 to Host Cell Protein ALIX and Increased Replication Fitness. J Virol. 2019;93: pubmed publisher
Magliozzi R, Carrero Z, Low T, Yuniati L, Valdes Quezada C, Kruiswijk F, et al. Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1. Cell Rep. 2018;23:3381-3391.e4 pubmed publisher
Martins A, Pfirrmann T, Heessen S, Sundqvist G, Bulone V, Andréasson C, et al. Ssy5 is a signaling serine protease that exhibits atypical biogenesis and marked S1 specificity. J Biol Chem. 2018;293:8362-8378 pubmed publisher
Zeng F, Hua Y, Liu X, Liu S, Lao K, Zhang Z, et al. Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II. Mol Cell Biol. 2018;38: pubmed publisher
Pentimalli F, Forte I, Esposito L, Indovina P, Iannuzzi C, Alfano L, et al. RBL2/p130 is a direct AKT target and is required to induce apoptosis upon AKT inhibition in lung cancer and mesothelioma cell lines. Oncogene. 2018;37:3657-3671 pubmed publisher
Rojas Ríos P, Chartier A, Pierson S, Simonelig M. Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogene Cbl. EMBO J. 2017;36:3194-3211 pubmed publisher
Okumura F, Joo Okumura A, Obara K, Petersen A, Nishikimi A, Fukui Y, et al. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2. Mol Biol Cell. 2017;28:3532-3541 pubmed publisher
Schmidt O, Weyer Y, Fink M, Muller M, Weys S, Bindreither M, et al. Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast. FEBS Lett. 2017;591:2803-2815 pubmed publisher
Kiprowska M, Stepanova A, Todaro D, Galkin A, Haas A, Wilson S, et al. Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: Relevance to Alzheimer's disease. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1157-1170 pubmed publisher
Kawaai K, Ando H, Satoh N, Yamada H, Ogawa N, Hirose M, et al. Splicing variation of Long-IRBIT determines the target selectivity of IRBIT family proteins. Proc Natl Acad Sci U S A. 2017;114:3921-3926 pubmed publisher
Yuan W, Guo S, Gao J, Zhong M, Yan G, Wu W, et al. General Control Nonderepressible 2 (GCN2) Kinase Inhibits Target of Rapamycin Complex 1 in Response to Amino Acid Starvation in Saccharomyces cerevisiae. J Biol Chem. 2017;292:2660-2669 pubmed publisher
Schiffmacher A, Xie V, Taneyhill L. Cadherin-6B proteolysis promotes the neural crest cell epithelial-to-mesenchymal transition through transcriptional regulation. J Cell Biol. 2016;215:735-747 pubmed
Webb B, White K, Grillo Hill B, Schönichen A, Choi C, Barber D. A Histidine Cluster in the Cytoplasmic Domain of the Na-H Exchanger NHE1 Confers pH-sensitive Phospholipid Binding and Regulates Transporter Activity. J Biol Chem. 2016;291:24096-24104 pubmed
Berquist B, Canugovi C, Sykora P, Wilson D, Bohr V. Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation. Nucleic Acids Res. 2012;40:8392-405 pubmed
Lefebvre F, Prouzet Mauleon V, Hugues M, Crouzet M, Vieillemard A, McCusker D, et al. Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites. Eukaryot Cell. 2012;11:590-600 pubmed publisher
Raspelli E, Cassani C, Lucchini G, Fraschini R. Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization. Mol Biol Cell. 2011;22:2185-97 pubmed publisher
Clifford D, Wolfe B, Roberts Galbraith R, McDonald W, Yates J, Gould K. The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. J Cell Biol. 2008;181:79-88 pubmed publisher
Olzmann J, Li L, Chudaev M, Chen J, Perez F, Palmiter R, et al. Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6. J Cell Biol. 2007;178:1025-38 pubmed
Phalen T, Weirather K, Deming P, Anathy V, Howe A, van Der Vliet A, et al. Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery. J Cell Biol. 2006;175:779-89 pubmed
Fraschini R, D Ambrosio C, Venturetti M, Lucchini G, Piatti S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol. 2006;172:335-46 pubmed
Zacharek S, Xiong Y, Shumway S. Negative regulation of TSC1-TSC2 by mammalian D-type cyclins. Cancer Res. 2005;65:11354-60 pubmed
Sarkar S, Floto R, Berger Z, Imarisio S, Cordenier A, Pasco M, et al. Lithium induces autophagy by inhibiting inositol monophosphatase. J Cell Biol. 2005;170:1101-11 pubmed
Ehrenreiter K, Piazzolla D, Velamoor V, Sobczak I, Small J, Takeda J, et al. Raf-1 regulates Rho signaling and cell migration. J Cell Biol. 2005;168:955-64 pubmed
Ricke R, Bielinsky A. Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Mol Cell. 2004;16:173-85 pubmed
Goel H, Fornaro M, Moro L, Teider N, Rhim J, King M, et al. Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins. J Cell Biol. 2004;166:407-18 pubmed
Vindis C, Cerretti D, Daniel T, Huynh Do U. EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis. J Cell Biol. 2003;162:661-71 pubmed
Slack Davis J, Eblen S, Zecevic M, Boerner S, Tarcsafalvi A, Diaz H, et al. PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation. J Cell Biol. 2003;162:281-91 pubmed
Zhang H, Webb D, Asmussen H, Horwitz A. Synapse formation is regulated by the signaling adaptor GIT1. J Cell Biol. 2003;161:131-42 pubmed
Lavoie B, Hogan E, Koshland D. In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin. J Cell Biol. 2002;156:805-15 pubmed
Lindsay M, Holaska J, Welch K, Paschal B, Macara I. Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export. J Cell Biol. 2001;153:1391-402 pubmed
Helliwell S, Losko S, Kaiser C. Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease. J Cell Biol. 2001;153:649-62 pubmed
Cronin S, Khoury A, Ferry D, Hampton R. Regulation of HMG-CoA reductase degradation requires the P-type ATPase Cod1p/Spf1p. J Cell Biol. 2000;148:915-24 pubmed
Ahmed N, Grimes H, Bellacosa A, Chan T, Tsichlis P. Transduction of interleukin-2 antiapoptotic and proliferative signals via Akt protein kinase. Proc Natl Acad Sci U S A. 1997;94:3627-32 pubmed
Alessi D, Andjelkovic M, Caudwell B, Cron P, Morrice N, Cohen P, et al. Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J. 1996;15:6541-51 pubmed
Meng X, Devin J, Sullivan W, Toft D, Baulieu E, Catelli M. Mutational analysis of Hsp90 alpha dimerization and subcellular localization: dimer disruption does not impede "in vivo' interaction with estrogen receptor. J Cell Sci. 1996;109 ( Pt 7):1677-87 pubmed
product information
Product Type :
Antibody
Product Name :
HA Tag Monoclonal Antibody (12CA5)
Catalog # :
MA1-12429
Quantity :
100 ug
Price :
US 478.00
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Tag
Applications :
ELISA: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Species :
Tag
Clone :
12CA5
Isotype :
IgG2b
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
Immunogen :
9 amino acid peptide to influenza virus hemagglutinin.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Aliases :
HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA