Cdc25A Monoclonal Antibody (DCS121) | Invitrogen MA1-12293 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cdc25A Monoclonal Antibody (DCS121)

catalog :

MA1-12293

quantity :

100 µg

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS121

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 5	Neumann J, Yang Y, KÃ¶hler R, Giaisi M, Witzens Harig M, Liu D, et al. Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest. Int J Cancer. 2015;137:2739-48 pubmed publisher
immunohistochemistry; human; 1:500 western blot; human; 1:200	Wang Z, Trope C, Flørenes V, Suo Z, Nesland J, Holm R. Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes. BMC Cancer. 2010;10:233 pubmed publisher
western blot; human	Zhang Y, Brognard J, Coughlin C, You Z, Dolled Filhart M, Aslanian A, et al. The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress. Mol Cell. 2009;35:442-53 pubmed publisher
western blot; human	Demidova A, Aau M, Zhuang L, Yu Q. Dual regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control. J Biol Chem. 2009;284:4132-9 pubmed publisher
immunohistochemistry; human; 1:20 western blot; human	Kim S, Lee S, Choi Y, Wang L, Park C, Shin Y. Extensive alteration in the expression profiles of TGFB pathway signaling components and TP53 is observed along the gastric dysplasia-carcinoma sequence. Histol Histopathol. 2008;23:1439-52 pubmed publisher
western blot; human	Johnson C, Esquela Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, et al. The let-7 microRNA represses cell proliferation pathways in human cells. Cancer Res. 2007;67:7713-22 pubmed
western blot; human	Berthet C, Raj K, Saudan P, Beard P. How adeno-associated virus Rep78 protein arrests cells completely in S phase. Proc Natl Acad Sci U S A. 2005;102:13634-9 pubmed
	Snow J, Murthy V, Dacus D, Hu C, Wallace N. β-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage. Pathogens. 2019;8: pubmed publisher
	Strom A, Hartman J, Foster J, Kietz S, Wimalasena J, Gustafsson J. Estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of the breast cancer cell line T47D. Proc Natl Acad Sci U S A. 2004;101:1566-71 pubmed
	Xiao Z, Chen Z, Gunasekera A, Sowin T, Rosenberg S, Fesik S, et al. Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents. J Biol Chem. 2003;278:21767-73 pubmed

product information

Product Type :

Antibody

Product Name :

Cdc25A Monoclonal Antibody (DCS121)

Catalog # :

MA1-12293

Quantity :

100 µg

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Human

Applications :

Immunoprecipitation: 1-10 µg/mL, Western Blot: Assay-dependent

Species :

Human

Clone :

DCS121

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

Immunogen :

Purified recombinant CDC25A protein.

Format :

Liquid

Applications w/Dilutions :

Immunoprecipitation: 1-10 µg/mL, Western Blot: Assay-dependent

Aliases :

CDC25 isoform A1-CAG; CDC25A; CDC25A2; CDC25A2-CAG isoform; Cdc25m3; cell division cycle 25A; D9Ertd393e; dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1

more info or order :

Invitrogen product webpage