Bcl-2 Monoclonal Antibody (10C4) | Invitrogen MA1-12246 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bcl-2 Monoclonal Antibody (10C4)

catalog :

MA1-12246

quantity :

100 µg

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C4

reactivity :

mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6d	Visekruna A, Hartmann S, Sillke Y, Glauben R, Fischer F, Raifer H, et al. Intestinal development and homeostasis require activation and apoptosis of diet-reactive T cells. J Clin Invest. 2019;129:1972-1983 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 5e	Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
flow cytometry; mouse; loading ...	Saha A, O Connor R, Thangavelu G, Lovitch S, Dandamudi D, Wilson C, et al. Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality. J Clin Invest. 2016;126:2642-60 pubmed publisher
western blot; rat	Xu H, Zhu G, Tian Y. Protective effects of trimetazidine on bone marrow mesenchymal stem cells viability in an ex vivo model of hypoxia and in vivo model of locally myocardial ischemia. J Huazhong Univ Sci Technolog Med Sci. 2012;32:36-41 pubmed publisher
western blot; rat; 1:1000	Baquedano E, García Cáceres C, Diz Chaves Y, Lagunas N, Calmarza Font I, Azcoitia I, et al. Prenatal stress induces long-term effects in cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats. PLoS ONE. 2011;6:e27549 pubmed publisher
western blot; mouse	Hirai H, Verma M, Watanabe S, Tastad C, Asakura Y, Asakura A. MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3. J Cell Biol. 2010;191:347-65 pubmed publisher
western blot; rat; 1:1000	Delgado Rubín A, Chowen J, Argente J, Frago L. Growth hormone-releasing peptide 6 protection of hypothalamic neurons from glutamate excitotoxicity is caspase independent and not mediated by insulin-like growth factor I. Eur J Neurosci. 2009;29:2115-24 pubmed publisher
western blot; rat; 1:200	Nahas Z, Jiang Y, Zeidan Y, Bielawska A, Szulc Z, DeVane L, et al. Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats. Behav Brain Res. 2009;197:41-4 pubmed publisher
western blot; mouse	Götschel F, Kern C, Lang S, Sparna T, Markmann C, Schwager J, et al. Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis. Exp Cell Res. 2008;314:1351-66 pubmed publisher
western blot; mouse	Schneider L, Essmann F, Kletke A, Rio P, Hanenberg H, Schulze Osthoff K, et al. TACC3 depletion sensitizes to paclitaxel-induced cell death and overrides p21WAF-mediated cell cycle arrest. Oncogene. 2008;27:116-25 pubmed
western blot; rat; 1:1000	Delgado Rubín de Célix A, Chowen J, Argente J, Frago L. Growth hormone releasing peptide-6 acts as a survival factor in glutamate-induced excitotoxicity. J Neurochem. 2006;99:839-49 pubmed
immunocytochemistry; rat; 1:100	Chai Y, Niu L, Sun X, Ding J, Hu G. Iptakalim protects PC12 cell against H2O2-induced oxidative injury via opening mitochondrial ATP-sensitive potassium channel. Biochem Biophys Res Commun. 2006;350:307-14 pubmed
western blot; mouse	López Diazguerrero N, López Araiza H, Conde Perezprina J, Bucio L, Cárdenas Aguayo M, Ventura J, et al. Bcl-2 protects against oxidative stress while inducing premature senescence. Free Radic Biol Med. 2006;40:1161-9 pubmed
immunohistochemistry - frozen section; rat; 2.5 ug/ml; fig 7	Yamanaka T, Helgeland L, Farstad I, Fukushima H, Midtvedt T, Brandtzaeg P. Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches. J Immunol. 2003;170:816-22 pubmed
immunocytochemistry; rat; 1:100; fig 1 western blot; rat; 1:1000; fig 1	Kaufmann T, Schlipf S, Sanz J, Neubert K, Stein R, Borner C. Characterization of the signal that directs Bcl-x(L), but not Bcl-2, to the mitochondrial outer membrane. J Cell Biol. 2003;160:53-64 pubmed
	Tan L, Qu W, Wu D, Liu M, Ai Q, Hu H, et al. The interferon regulatory factor 6 promotes cisplatin sensitivity in colorectal cancer. Bioengineered. 2022;13:10504-10517 pubmed publisher
	Niogret C, Miah S, Rota G, Fonta N, Wang H, Held W, et al. Shp-2 is critical for ERK and metabolic engagement downstream of IL-15 receptor in NK cells. Nat Commun. 2019;10:1444 pubmed publisher
	Mayorga M, Bahi N, Ballester M, Comella J, Sanchis D. Bcl-2 is a key factor for cardiac fibroblast resistance to programmed cell death. J Biol Chem. 2004;279:34882-9 pubmed
	Putcha G, Harris C, Moulder K, Easton R, Thompson C, Johnson E. Intrinsic and extrinsic pathway signaling during neuronal apoptosis: lessons from the analysis of mutant mice. J Cell Biol. 2002;157:441-53 pubmed

product information

Product Type :

Antibody

Product Name :

Bcl-2 Monoclonal Antibody (10C4)

Catalog # :

MA1-12246

Quantity :

100 µg

Price :

US 446.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

Western Blot: 1-10 µg/mL

Species :

Mouse, Rat

Clone :

10C4

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Immunogen :

Synthetic peptide corresponding to residues 61-76 of mouse bcl-2.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1-10 µg/mL

Aliases :

apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50

more info or order :

Invitrogen product webpage