This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Bcl-xL Monoclonal Antibody (2H12)
catalog :
MA1-12241
quantity :
100µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2H12
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 29
Published Application/Species/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:50; tbl 2
Sjöström J, Blomqvist C, von Boguslawski K, Bengtsson N, Mjaaland I, Malmström P, et al. The predictive value of bcl-2, bax, bcl-xL, bag-1, fas, and fasL for chemotherapy response in advanced breast cancer. Clin Cancer Res. 2002;8:811-6 pubmed
  • western blot; human; 0.5 ug/ml; fig 5
Li K, Pang J, Lau K, Zhou L, Mao Y, Wang Y, et al. MiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3). Brain Pathol. 2013;23:413-25 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:10
Alonso S, Ortiz P, Pollan M, Perez-Gomez B, Sanchez L, Acuña M, et al. Progression in cutaneous malignant melanoma is associated with distinct expression profiles: a tissue microarray-based study. Am J Pathol. 2004;164:193-203 pubmed
  • immunohistochemistry - paraffin section; human; 1:20; fig 3b
Kim L, Nadarajah V, Peh S, Poppema S. Expression of Bcl-2 family members and presence of Epstein-Barr virus in the regulation of cell growth and death in classical Hodgkin's lymphoma. Histopathology. 2004;44:257-67 pubmed
  • western blot; human; fig 1
Nuutinen U, Simelius N, Ropponen A, Eeva J, Mättö M, Eray M, et al. PDTC enables type I TRAIL signaling in type II follicular lymphoma cells. Leuk Res. 2009;33:829-36 pubmed publisher
  • immunohistochemistry; human; 1:150; fig 1-4
Korkolopoulou P, Lazaris A, Konstantinidou A, Kavantzas N, Patsouris E, Christodoulou P, et al. Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival. Eur Urol. 2002;41:274-83 pubmed
  • western blot; human; fig 4
Goetzl E, Kong Y, Mei B. Lysophosphatidic acid and sphingosine 1-phosphate protection of T cells from apoptosis in association with suppression of Bax. J Immunol. 1999;162:2049-56 pubmed
  • immunohistochemistry - paraffin section; human; 1:10
Garcia J, Mollejo M, Fraga M, Forteza J, Muniesa J, Perez-Guillermo M, et al. Large B-cell lymphoma with Hodgkin's features. Histopathology. 2005;47:101-10 pubmed
  • western blot; human
Nuutinen U, Ropponen A, Eeva J, Eray M, Pellinen R, Wahlfors J, et al. The effect of microenvironmental CD40 signals on TRAIL- and drug-induced apoptosis in follicular lymphoma cells. Scand J Immunol. 2009;70:565-73 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 2C
Takei H, Buckleair L, Powell S. Immunohistochemical expression of apoptosis regulating proteins and sex hormone receptors in meningiomas. Neuropathology. 2008;28:62-8 pubmed
  • western blot; human; 1:200
Kobayashi S, Lee S, Meng X, Mott J, Bronk S, Werneburg N, et al. Serine 64 phosphorylation enhances the antiapoptotic function of Mcl-1. J Biol Chem. 2007;282:18407-17 pubmed
  • western blot; human
Holland H, Koschny R, Krupp W, Meixensberger J, Bauer M, Schober R, et al. Cytogenetic and molecular biological characterization of an adult medulloblastoma. Cancer Genet Cytogenet. 2007;178:104-13 pubmed
  • immunohistochemistry; human
Hyjek E, Chadburn A, Liu Y, Cesarman E, Knowles D. BCL-6 protein is expressed in precursor T-cell lymphoblastic lymphoma and in prenatal and postnatal thymus. Blood. 2001;97:270-6 pubmed
  • western blot; mouse
Gali-Muhtasib H, Abou Kheir W, Kheir L, Darwiche N, Crooks P. Molecular pathway for thymoquinone-induced cell-cycle arrest and apoptosis in neoplastic keratinocytes. Anticancer Drugs. 2004;15:389-99 pubmed
  • immunohistochemistry - paraffin section; human; 1:10
Garcia J, Camacho F, Morente M, Fraga M, Montalban C, Alvaro T, et al. Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays. Blood. 2003;101:681-9 pubmed
  • immunohistochemistry - paraffin section; human; 1:10; tbl 1
Sáez A, Sáez A, Artiga M, Pérez-Rosado A, Camacho F, Diez A, et al. Building an outcome predictor model for diffuse large B-cell lymphoma. Am J Pathol. 2004;164:613-22 pubmed
  • immunocytochemistry; rat; 1:100; fig 1
Lindenboim L, Schlipf S, Kaufmann T, Borner C, Stein R. Bcl-x(S) induces an NGF-inhibitable cytochrome c release. Exp Cell Res. 2004;297:392-403 pubmed
  • western blot; human
Koschny R, Ganten T, Sykora J, Haas T, Sprick M, Kolb A, et al. TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window. Hepatology. 2007;45:649-58 pubmed
  • immunohistochemistry - paraffin section; human; fig 1
Dawson M, Opat S, Taouk Y, Donovan M, Zammit M, Monaghan K, et al. Clinical and immunohistochemical features associated with a response to bortezomib in patients with multiple myeloma. Clin Cancer Res. 2009;15:714-22 pubmed publisher
  • western blot; mouse
Götschel F, Kern C, Lang S, Sparna T, Markmann C, Schwager J, et al. Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis. Exp Cell Res. 2008;314:1351-66 pubmed publisher
  • immunohistochemistry; human; 1:100
Hsieh P, Olsen R, O'Malley D, Konoplev S, Hussong J, Dunphy C, et al. The role of Janus Kinase 2 V617F mutation in extramedullary hematopoiesis of the spleen in neoplastic myeloid disorders. Mod Pathol. 2007;20:929-35 pubmed
  • immunohistochemistry - paraffin section; human
Lahat G, Tuvin D, Wei C, Wang W, Pollock R, Anaya D, et al. Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study. Ann Oncol. 2010;21:1112-20 pubmed publisher
  • immunohistochemistry; human; 1:25; fig 3
Rassidakis G, Jones D, Lai R, Ramalingam P, Sarris A, McDonnell T, et al. BCL-2 family proteins in peripheral T-cell lymphomas: correlation with tumour apoptosis and proliferation. J Pathol. 2003;200:240-8 pubmed
Schulze-Bergkamen H, Ehrenberg R, Hickmann L, Vick B, Urbanik T, Schimanski C, et al. Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells. World J Gastroenterol. 2008;14:3829-40 pubmed
Ferrero E, Belloni D, Contini P, Foglieni C, Ferrero M, Fabbri M, et al. Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation. Blood. 2003;101:186-93 pubmed
Wang J, Wei Q, Wang C, Hill W, Hess D, Dong Z. Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria. J Biol Chem. 2004;279:19948-54 pubmed
Shaknovich R, Basso K, Bhagat G, Mansukhani M, Hatzivassiliou G, Murty V, et al. Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling. Haematologica. 2006;91:1313-20 pubmed
Sharif M, Whitehouse A, Sharman P, Perry M, Adams M. Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3. Arthritis Rheum. 2004;50:507-15 pubmed
Dong Z, Wang J. Hypoxia selection of death-resistant cells. A role for Bcl-X(L). J Biol Chem. 2004;279:9215-21 pubmed
product information
Product Type :
Antibody
Product Name :
Bcl-xL Monoclonal Antibody (2H12)
Catalog # :
MA1-12241
Quantity :
100µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Mouse, Rat
Applications :
Western Blot: 1 - 5 ug/ml
Species :
Mouse, Rat
Clone :
2H12
Isotype :
IgG2a
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Bcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells.
Immunogen :
Synthetic peptide corresponding to residues 3-14 of the human bcl-xl.
Format :
Liquid
Applications w/Dilutions :
Western Blot: 1 - 5 ug/ml
Aliases :
BCL-XL/S, BCL2L, BCLX, Bcl-X, DKFZp781P2092, bcl-2 L1, bcl-xS
PubMed References :
11133771, 11895913, 12180228, 12393683, 12393747, 12754745, 14676192, 14695333, 14742266, 14872493, 14987230, 15004018, 15057144, 15212942, 15982329, 17018379, 17326159, 17463001, 17643100, 17954265, 18021195, 18261723, 18609706, 18977530, 19147779, 19875755, 19906199, 23227829, 9973477
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA