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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HMOX1 Monoclonal Antibody (HO-1-1)
catalog :
MA1-112
quantity :
200 µg
price :
US 496.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HO-1-1
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; fig s7b
  • western blot; human; fig 3b
Wang G, Zhou H, Strulovici Barel Y, Al Hijji M, Ou X, Salit J, et al. Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium. Autophagy. 2017;13:1205-1220 pubmed publisher
  • western blot; rat; loading ...; fig 4a
Chen Z, Wang Q. Activation of PPAR? by baicalin attenuates pulmonary hypertension in an infant rat model by suppressing HMGB1/RAGE signaling. FEBS Open Bio. 2017;7:477-484 pubmed publisher
  • western blot; mouse; 1:500; fig 5a
Lee J, Kim H, Han J, Kim Y, Son C. Anti-fatigue effect of Myelophil in a chronic forced exercise mouse model. Eur J Pharmacol. 2015;764:100-8 pubmed publisher
  • western blot; mouse; 1:500
Hong S, Lee J, Lee J, Lee H, Kim H, Lee S, et al. The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model. J Ethnopharmacol. 2015;168:268-78 pubmed publisher
Fagone P, Piombino E, Mangano K, De Pasquale R, Nicoletti F, Caltabiano R. Evaluation of the Involvement of Heme Oxygenase-1 Expression in Discoid Lupus Erythematosus Lesions. Antioxidants (Basel). 2023;12: pubmed publisher
Kong W, Zhou W, He Z, Zhang X, Li S, Zhong R, et al. Polymerized human cord hemoglobin assisted with ascorbic acid as a red blood cell substitute alleviating oxidative stress for blood transfusion. Front Bioeng Biotechnol. 2023;11:1151975 pubmed publisher
Chen H, Nguyen N, Magtoto C, Cobbold S, Bidgood G, Meza Guzman L, et al. Design and characterization of a heterobifunctional degrader of KEAP1. Redox Biol. 2023;59:102552 pubmed publisher
Zhang X, Akcan E, Correia M, Rameika N, Kundu S, Stoimenov I, et al. Enhanced cytotoxicity of a novel family of ATPase inhibitors in colorectal cancer cells with high NAT2 activity. Biochem Pharmacol. 2022;203:115184 pubmed publisher
Ren R, Fang Y, Sherchan P, Lu Q, Lenahan C, Zhang J, et al. Kynurenine/Aryl Hydrocarbon Receptor Modulates Mitochondria-Mediated Oxidative Stress and Neuronal Apoptosis in Experimental Intracerebral Hemorrhage. Antioxid Redox Signal. 2022;37:1111-1129 pubmed publisher
Soliman S, El Labbad E, Abu Qiyas A, Fayed B, Hamoda A, Al Rawi A, et al. Novel Secreted Peptides From Rhizopus arrhizus var. delemar With Immunomodulatory Effects That Enhance Fungal Pathogenesis. Front Microbiol. 2022;13:863133 pubmed publisher
Lawrence M, Elhendawi M, Morlock M, Liu W, Liu S, Palakkan A, et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity. iScience. 2022;25:103884 pubmed publisher
Lin Y, Lin C, Ho E, Ke Y, Petridi S, Elliott C, et al. Glial Nrf2 signaling mediates the neuroprotection exerted by Gastrodia elata Blume in Lrrk2-G2019S Parkinson's disease. elife. 2021;10: pubmed publisher
Wu Y, Liang S, Zhu H, Zhu Y. Analysis of immune-related key genes in Alzheimer's disease. Bioengineered. 2021;12:9610-9624 pubmed publisher
Ebrahimpour A, Wang M, Li L, Jegga A, Bonnen M, Eissa N, et al. Esomeprazole attenuates inflammatory and fibrotic response in lung cells through the MAPK/Nrf2/HO1 pathway. J Inflamm (Lond). 2021;18:17 pubmed publisher
Fige É, Szendrei J, Sós L, Kraszewska I, Potor L, Balla J, et al. Heme Oxygenase-1 Contributes to Both the Engulfment and the Anti-Inflammatory Program of Macrophages during Efferocytosis. Cells. 2021;10: pubmed publisher
Eassawy M, Salem A, Ismail A. Biochemical study on the protective effect of curcumin on acetaminophen and gamma-irradiation induced hepatic toxicity in rats. Environ Toxicol. 2020;: pubmed publisher
Lombard D, Kohler W, Guo A, Gendron C, Han M, Ding W, et al. High-throughput small molecule screening reveals Nrf2-dependent and -independent pathways of cellular stress resistance. Sci Adv. 2020;6: pubmed publisher
De Nuccio C, Bernardo A, Troiano C, Brignone M, Falchi M, Greco A, et al. NRF2 and PPAR-γ Pathways in Oligodendrocyte Progenitors: Focus on ROS Protection, Mitochondrial Biogenesis and Promotion of Cell Differentiation. Int J Mol Sci. 2020;21: pubmed publisher
Chen W, Hu S, Mao S, Xu Y, Guo H, Li H, et al. Discovery of Mitochondrial Transcription Inhibitors Active in Pancreatic Cancer Cells. ChemMedChem. 2020;15:2029-2039 pubmed publisher
Yi W, Lan H, Wen Y, Wang Y, He D, Bai Z, et al. HO-1 overexpression alleviates senescence by inducing autophagy via the mitochondrial route in human nucleus pulposus cells. J Cell Physiol. 2020;: pubmed publisher
Wiel C, Le Gal K, Ibrahim M, Jahangir C, Kashif M, Yao H, et al. BACH1 Stabilization by Antioxidants Stimulates Lung Cancer Metastasis. Cell. 2019;: pubmed publisher
Gill N, Leng Y, Romero R, Xu Y, Panaitescu B, Miller D, et al. The immunophenotype of decidual macrophages in acute atherosis. Am J Reprod Immunol. 2019;81:e13098 pubmed publisher
Forootan S, Mutter F, Kipar A, Iwawaki T, Francis B, Goldring C, et al. Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity. Sci Rep. 2017;7:16084 pubmed publisher
Nguyen Ho Bouldoires T, Clapéron A, Mergey M, Wendum D, Desbois Mouthon C, Tahraoui S, et al. Mitogen-activated protein kinase-activated protein kinase 2 mediates resistance to hydrogen peroxide-induced oxidative stress in human hepatobiliary cancer cells. Free Radic Biol Med. 2015;89:34-46 pubmed publisher
image
image 1 :
Invitrogen MA1-112 image 1
Immunofluorescent analysis of Heme Oxygenase-1 (HO-1, green) in HeLa cells either left untreated (left panel) or treated with 0.5mM copper sulfate (right panel) for 24 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an HO-1 monoclonal antibody (Product # MA1-112) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
product information
Product Type :
Antibody
Product Name :
HMOX1 Monoclonal Antibody (HO-1-1)
Catalog # :
MA1-112
Quantity :
200 µg
Price :
US 496.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Bovine, Canine, Human, Mouse, Rat
Applications :
Flow Cytometry: 10 µg/mL, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:1,000
Species :
Bovine, Canine, Human, Mouse, Rat
Clone :
HO-1-1
Isotype :
IgG1, kappa
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
Immunogen :
Synthetic peptide corresponding to the sequence near the N-terminus (amino acids 1-30) of human Heme Oxygenase 1
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 10 µg/mL, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 1:1,000
Aliases :
bK286B10; D8Wsu38e; heat shock protein 32; heat shock protein, 32-kD; heme orygenase 1; heme oxygenase; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; heme oxygenase 1; heme oxygenase-1; Hemox; hemoxygenase; Heox; HEOXG; Hmox; Hmox1; HMOX1D; HO; Ho1; HO-1; Hsp32; P32 protein
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA