product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Nestin Monoclonal Antibody (10C2)
catalog :
MA1-110
quantity :
100 µg
price :
US 500.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10C2
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:200; fig s5
Soldati C, Lopez Fabuel I, Wanderlingh L, García Macia M, Monfregola J, Esposito A, et al. Repurposing of tamoxifen ameliorates CLN3 and CLN7 disease phenotype. EMBO Mol Med. 2021;13:e13742 pubmed publisher
  • immunocytochemistry; human; 1:250; fig s13c
Truong D, Phlairaharn T, Eßwein B, Gruber C, Tümen D, Baligács E, et al. Non-invasive and high-throughput interrogation of exon-specific isoform expression. Nat Cell Biol. 2021;23:652-663 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 2b
Mendivil Perez M, Soto Mercado V, Guerra Librero A, Fernandez Gil B, Florido J, Shen Y, et al. Melatonin enhances neural stem cell differentiation and engraftment by increasing mitochondrial function. J Pineal Res. 2017;63: pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:300; loading ...; tbl 2
Mesa H, Gilles S, Datta M, Murugan P, Larson W, Dachel S, et al. Comparative immunomorphology of testicular Sertoli and sertoliform tumors. Hum Pathol. 2017;61:181-189 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig 1g
  • immunocytochemistry; human; 1:50; fig 2e
Abe S, Yamaguchi S, Sato Y, Harada K. Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells. Stem Cells Transl Med. 2016;5:117-28 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:25; fig 1
Luo W, Li S, Peng B, Ye Y, Deng X, Yao K. Embryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma. PLoS ONE. 2013;8:e56324 pubmed publisher
  • immunohistochemistry; human; 1:20
Claassen H, Mönig H, Sel S, Werner J, Paulsen F. Androgen receptors and gender-specific distribution of alkaline phosphatase in human thyroid cartilage. Histochem Cell Biol. 2006;126:381-8 pubmed
Shin K, Ali G, Ali Moussa H, Gupta V, de la Fuente A, Kim H, et al. Deletion of TRPC6, an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells. Mol Neurobiol. 2023;60:7297-7308 pubmed publisher
Jiang X, Liu S, Fu Y, Liu X, Li X, Yang B, et al. Human cytomegalovirus infection perturbs neural progenitor cell fate via the expression of viral microRNAs. J Med Virol. 2023;95:e28574 pubmed publisher
Kim J, Kim Y, Kim J, Lee S, Kim M, Jekarl D. Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient. Int J Mol Sci. 2023;24: pubmed publisher
Hou H, Wang Y, Yang L, Wang Y. Exosomal miR-128-3p reversed fibrinogen-mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemia. CNS Neurosci Ther. 2023;29:1405-1422 pubmed publisher
Villarroya Beltri C, Martins A, Garc xed a A, Gim xe9 nez D, Zarzuela E, Novo M, et al. Mammalian CDC14 phosphatases control exit from stemness in pluripotent cells. EMBO J. 2023;42:e111251 pubmed publisher
Lakis N, Brodsky A, Karashchuk G, Audesse A, Yang D, Sturtevant A, et al. Stem cell phenotype predicts therapeutic response in glioblastomas with MGMT promoter methylation. Acta Neuropathol Commun. 2022;10:159 pubmed publisher
Schmidt S, Luecken M, Tr xfc mbach D, Hembach S, Niedermeier K, Wenck N, et al. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease. Nat Commun. 2022;13:4819 pubmed publisher
Larrieu C, Storevik S, Guyon J, Pagano Zottola A, Bouchez C, Derieppe M, et al. Refining the Role of Pyruvate Dehydrogenase Kinases in Glioblastoma Development. Cancers (Basel). 2022;14: pubmed publisher
Warrier N, Krishnan R, Prabhu V, Hariharapura R, Agarwal P, Kumar P. Survivin Inhibition by Piperine Sensitizes Glioblastoma Cancer Stem Cells and Leads to Better Drug Response. Int J Mol Sci. 2022;23: pubmed publisher
Liu X, Li C, Li J, Xie L, Hong Z, Zheng K, et al. EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes. Mol Med. 2022;28:50 pubmed publisher
Essien E, Hofer T, Atkinson M, Anastasov N. Combining HDAC and MEK Inhibitors with Radiation against Glioblastoma-Derived Spheres. Cells. 2022;11: pubmed publisher
Rimkus T, Arrigo A, Zhu D, Carpenter R, Sirkisoon S, Doheny D, et al. NEDD4 degrades TUSC2 to promote glioblastoma progression. Cancer Lett. 2022;531:124-135 pubmed publisher
Barnts K, Feng J, Qin C, Zhang H, Cheng Y. Adenomatoid odontogenic tumor: evidence for a mixed odontogenic tumor. Oral Surg Oral Med Oral Pathol Oral Radiol. 2022;133:675-683 pubmed publisher
LaForce G, Farr J, Liu J, Akesson C, Gumus E, Pinkard O, et al. Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. Neuron. 2022;110:1340-1357.e7 pubmed publisher
Lopez Fabuel I, García Macia M, Buondelmonte C, Burmistrova O, Bonora N, Alonso Batan P, et al. Aberrant upregulation of the glycolytic enzyme PFKFB3 in CLN7 neuronal ceroid lipofuscinosis. Nat Commun. 2022;13:536 pubmed publisher
Zyner K, Simeone A, Flynn S, Doyle C, Marsico G, Adhikari S, et al. G-quadruplex DNA structures in human stem cells and differentiation. Nat Commun. 2022;13:142 pubmed publisher
Hong S, Park Y, Lee J, Moon J, Kong E, Jeon J, et al. 3D Visualization of Dynamic Cellular Reaction of Pulpal CD11c+ Dendritic Cells against Pulpitis in Whole Murine Tooth. Int J Mol Sci. 2021;22: pubmed publisher
Frosina G, Fontana V, Verzola D, Rosa A, Gaggero G, Garibotto G, et al. Ultra-hyper-fractionated radiotherapy for high-grade gliomas. J Neurosci Res. 2021;99:3182-3203 pubmed publisher
Kimura K, Koizumi T, Urasawa T, Ohta Y, Takakura D, Kawasaki N. Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells. Sci Rep. 2021;11:11169 pubmed publisher
Fan W, Tang S, Fan X, Fang Y, Xu X, Li L, et al. SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B. elife. 2021;10: pubmed publisher
Park Y, Son C, Seo Y, Lee Y, Har A, Park J. CPNE7-Induced Autophagy Restores the Physiological Function of Mature Odontoblasts. Front Cell Dev Biol. 2021;9:655498 pubmed publisher
Theunissen F, Anderton R, Mastaglia F, Flynn L, Winter S, James I, et al. Novel STMN2 Variant Linked to Amyotrophic Lateral Sclerosis Risk and Clinical Phenotype. Front Aging Neurosci. 2021;13:658226 pubmed publisher
Challagundla N, Agrawal Rajput R. microRNAs (miR 9, 124, 155 and 224) transdifferentiate mouse macrophages to neurons. Exp Cell Res. 2021;402:112563 pubmed publisher
Ahn L, Coatti G, Liu J, Gumus E, Schaffer A, Miranda H. An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model. J Neurosci Res. 2021;99:110-123 pubmed publisher
Renner H, Grabos M, Becker K, Kagermeier T, Wu J, Otto M, et al. A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids. elife. 2020;9: pubmed publisher
Saxena S, Choudhury S, Mohan K. Reproducible differentiation and characterization of neurons from mouse embryonic stem cells. MethodsX. 2020;7:101073 pubmed publisher
Long Y, Hwang T, Gooding A, Goodrich K, Rinn J, Cech T. RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells. Nat Genet. 2020;: pubmed publisher
Breen M, Browne A, Hoffman G, Stathopoulos S, Brennand K, Buxbaum J, et al. Transcriptional signatures of participant-derived neural progenitor cells and neurons implicate altered Wnt signaling in Phelan-McDermid syndrome and autism. Mol Autism. 2020;11:53 pubmed publisher
Sharma Y, Saha S, Joseph A, Krishnan H, Raghu P. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res. 2020;5:16 pubmed publisher
Morrow C, Porter T, Xu N, Arndt Z, Ako Asare K, Heo H, et al. Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. Cell Stem Cell. 2020;26:558-568.e9 pubmed publisher
Lingasamy P, Tobi A, Haugas M, Hunt H, Paiste P, Asser T, et al. Bi-specific tenascin-C and fibronectin targeted peptide for solid tumor delivery. Biomaterials. 2019;219:119373 pubmed publisher
Li T, Zheng Y, Li Y, Ye D. Comparison of in vitro Neuronal Differentiation Capacity Between Mouse Epiblast Stem Cells Derived From Nuclear Transfer and Naturally Fertilized Embryos. Front Mol Neurosci. 2018;11:392 pubmed publisher
Mullins C, Schneider B, Stockhammer F, Krohn M, Classen C, Linnebacher M. Establishment and characterization of primary glioblastoma cell lines from fresh and frozen material: a detailed comparison. PLoS ONE. 2013;8:e71070 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Nestin Monoclonal Antibody (10C2)
Catalog # :
MA1-110
Quantity :
100 µg
Price :
US 500.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:100, Western Blot: 1:500-1:1,500
Species :
Human, Mouse
Clone :
10C2
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
Immunogen :
Human nestin protein corresponding to residues 1464-1614
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1 µg/test, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:100, Western Blot: 1:500-1:1,500
Aliases :
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA