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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Cytokeratin 18 Monoclonal Antibody (DC-10), FITC
catalog :
MA1-10327
quantity :
100 ug
price :
US 427.00
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
DC-10
reactivity :
African green monkey, human, mouse, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
Invitrogen product webpage
citations: 25
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, et al. A novel mechanism of keratin cytoskeleton organization through casein kinase I? and FAM83H in colorectal cancer. J Cell Sci. 2013;126:4721-31 pubmed publisher
  • immunoprecipitation; human
Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
  • immunohistochemistry; human; 1:200
Marian C, Boilă A, Soanca D, Malau M, Podeanu D, Resetkova E, et al. Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature. APMIS. 2013;121:272-9 pubmed publisher
  • immunohistochemistry; human; 1:500
Szponar A, Kovacs G. Expression of KRT7 and WT1 differentiates precursor lesions of Wilms' tumours from those of papillary renal cell tumours and mucinous tubular and spindle cell carcinomas. Virchows Arch. 2012;460:423-7 pubmed publisher
  • western blot; mouse
Singla A, Moons D, Snider N, Wagenmaker E, Jayasundera V, Omary M. Oxidative stress, Nrf2 and keratin up-regulation associate with Mallory-Denk body formation in mouse erythropoietic protoporphyria. Hepatology. 2012;56:322-31 pubmed publisher
  • western blot; mouse; 1:1000
Ku N, Toivola D, Strnad P, Omary M. Cytoskeletal keratin glycosylation protects epithelial tissue from injury. Nat Cell Biol. 2010;12:876-85 pubmed publisher
  • immunohistochemistry; human; 1:500
Robertson F, Ogasawara M, Ye Z, Chu K, Pickei R, Debeb B, et al. Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype. J Biomol Screen. 2010;15:820-9 pubmed publisher
  • western blot; human; 1:2000
Yalcin Ozuysal O, Fiche M, Guitierrez M, Wagner K, Raffoul W, Brisken C. Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates. Cell Death Differ. 2010;17:1600-12 pubmed publisher
  • immunohistochemistry; African green monkey
Wood C, Kaplan J, Fontenot M, Williams J, Cline J. Endometrial profile of tamoxifen and low-dose estradiol combination therapy. Clin Cancer Res. 2010;16:946-56 pubmed publisher
  • flow cytometry; human
Metallo C, Ji L, de Pablo J, Palecek S. Directed differentiation of human embryonic stem cells to epidermal progenitors. Methods Mol Biol. 2010;585:83-92 pubmed publisher
  • western blot; human; 1:200
Chang C, Chiou P, Chen W, Li Y, Yiu J, Cheng Y, et al. Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines. Res Vet Sci. 2010;88:285-93 pubmed publisher
  • immunohistochemistry; human
Yu G, Goodloe S, D Angelis C, McGrath B, Chen F. Giant clear cell hidradenoma of the knee. J Cutan Pathol. 2010;37:e37-41 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Røsland G, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res. 2009;69:5331-9 pubmed publisher
  • ELISA; human; 10 ug/ml
Tao G, Li D, Zhou Q, Toivola D, Strnad P, Sandesara N, et al. Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation. J Pathol. 2008;215:164-74 pubmed publisher
  • immunohistochemistry; rhesus macaque; 1:100
Wood C, Hester J, Cline J. Mammary gland development in early pubertal female macaques. Toxicol Pathol. 2007;35:795-805 pubmed
  • western blot; human
Tao G, Strnad P, Zhou Q, Kamal A, Zhang L, Madani N, et al. Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease. Clin Gastroenterol Hepatol. 2007;5:857-64 pubmed
  • immunohistochemistry; human
Gurbuz Y, Kose N, Aydin O, Ozturk M. An immunohistochemical profile of giant cell carcinoma of the larynx. Auris Nasus Larynx. 2007;34:413-6 pubmed
  • western blot; human
Tao G, Toivola D, Zhou Q, Strnad P, Xu B, Michie S, et al. Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner. J Cell Sci. 2006;119:1425-32 pubmed
  • immunohistochemistry; human
Tao G, Nakamichi I, Ku N, Wang J, Frolkis M, Gong X, et al. Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res. 2006;312:411-22 pubmed
Doerfler R, Melamed J, Whitehead K. The Effect of Infant Gastric Digestion on Human Maternal Milk Cells. Mol Nutr Food Res. 2022;66:e2200090 pubmed publisher
Ku N, Toivola D, Zhou Q, Tao G, Zhong B, Omary M. Studying simple epithelial keratins in cells and tissues. Methods Cell Biol. 2004;78:489-517 pubmed
Toivola D, Ku N, Resurreccion E, Nelson D, Wright T, Omary M. Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease. Hepatology. 2004;40:459-66 pubmed
Apaydin R, Gurbuz Y, Bayramgurler D, Muezzinoglu B, Bilen N. Cytokeratin expression in lichen amyloidosus and macular amyloidosis. J Eur Acad Dermatol Venereol. 2004;18:305-9 pubmed
Ku N, Soetikno R, Omary M. Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury. Hepatology. 2003;37:1006-14 pubmed
Ku N, Gish R, Wright T, Omary M. Keratin 8 mutations in patients with cryptogenic liver disease. N Engl J Med. 2001;344:1580-7 pubmed
product information
Product Type :
Antibody
Product Name :
Cytokeratin 18 Monoclonal Antibody (DC-10), FITC
Catalog # :
MA1-10327
Quantity :
100 ug
Price :
US 427.00
Clonality :
Monoclonal
Purity :
Size-exclusion chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 1-5 ug/mL
Species :
Human
Clone :
DC-10
Isotype :
IgG1
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Immunogen :
Human breast carcinoma cell line PMC-42.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1-5 ug/mL
Aliases :
cb83; Cell proliferation-inducing gene 46 protein; CK 18; CK18; CK-18; CYK18; cytokeratin 18; cytokeratin endo B; cytokeratin type -1; DAPK-1; Cytokeratin18; cytokeratin-18; dapk1; dorsal aorta proneprin kinesin-1; DreK18; Endo B; I79_023185; K 18; K18; keratin 18; keratin 18, type I; keratin 18a, tandem duplicate 1; keratin complex 1, acidic, gene 18; keratin D; Keratin, type I cytoskeletal 18; Keratin18; keratin-18; Kerd; KRT 18; Krt1-18; KRT18; krt18 {ECO:0000250; krt18a.1; LOC100008885; PIG46; sb:cb83; type I cytoskeletal 18; UniProtKB:P05783}; wu:fa13f02; wu:fb36b04
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA