CD86 Monoclonal Antibody (GL-1) | Invitrogen MA1-10299 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD86 Monoclonal Antibody (GL-1)

catalog :

MA1-10299

quantity :

100 ug

price :

US 209.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

GL-1

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:200; loading ...; fig s10b	Stoff M, Ebbecke T, Ciurkiewicz M, Pavasutthipaisit S, Mayer Lambertz S, St xf6 rk T, et al. C-type lectin receptor DCIR contributes to hippocampal injury in acute neurotropic virus infection. Sci Rep. 2021;11:23819 pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Weng X, Zhao H, Guan Q, Shi G, Feng S, Gleave M, et al. Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys. Immunol Cell Biol. 2021;99:274-287 pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Ano Y, Ohya R, Takaichi Y, Washinuma T, Uchida K, Takashima A, et al. β-Lactolin, a Whey-Derived Lacto-Tetrapeptide, Prevents Alzheimer's Disease Pathologies and Cognitive Decline. J Alzheimers Dis. 2020;73:1331-1342 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b, 1e	Mizuno R, Sugiura D, Shimizu K, Maruhashi T, Watada M, Okazaki I, et al. PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Front Immunol. 2019;10:630 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Lee Y, Ju J, Shon W, Oh S, Min C, Kang M, et al. Skewed Dendritic Cell Differentiation of MyD88-Deficient Donor Bone Marrow Cells, Instead of Massive Expansion as Myeloid-Derived Suppressor Cells, Aggravates GVHD. Immune Netw. 2018;18:e44 pubmed publisher
flow cytometry; mouse; loading ...; fig e5c	Du X, Wen J, Wang Y, Karmaus P, Khatamian A, Tan H, et al. Hippo/Mst signalling couples metabolic state and immune function of CD8α+ dendritic cells. Nature. 2018;558:141-145 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Singla B, Ghoshal P, Lin H, Wei Q, Dong Z, Csanyi G. PKCδ-Mediated Nox2 Activation Promotes Fluid-Phase Pinocytosis of Antigens by Immature Dendritic Cells. Front Immunol. 2018;9:537 pubmed publisher
flow cytometry; mouse; fig 2d	Wasiuk A, Testa J, Weidlick J, Sisson C, Vitale L, Widger J, et al. CD27-Mediated Regulatory T Cell Depletion and Effector T Cell Costimulation Both Contribute to Antitumor Efficacy. J Immunol. 2017;199:4110-4123 pubmed publisher
flow cytometry; mouse; loading ...; fig 5d	Shrestha B, You D, Saravia J, Siefker D, Jaligama S, Lee G, et al. IL-4R? on dendritic cells in neonates and Th2 immunopathology in respiratory syncytial virus infection. J Leukoc Biol. 2017;102:153-161 pubmed publisher
flow cytometry; mouse; fig 3a	van der Vlugt L, Obieglo K, Ozir Fazalalikhan A, Sparwasser T, Haeberlein S, Smits H. Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. Int J Parasitol. 2017;47:545-554 pubmed publisher
flow cytometry; mouse; loading ...; fig 5d	Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig S8b	Srinivasan S, Su M, Ravishankar S, Moore J, Head P, Dixon J, et al. TLR-exosomes exhibit distinct kinetics and effector function. Sci Rep. 2017;7:41623 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Baranek T, Morello E, Valayer A, Aimar R, Bréa D, Henry C, et al. FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection. Front Immunol. 2017;8:123 pubmed publisher
immunohistochemistry; rat; 1:100; loading ...; fig 6a	Gomaa A, El Aziz E. Vitamin D reduces high-fat diet induced weight gain and C-reactive protein, increases interleukin-10, and reduces CD86 and caspase-3. Pathophysiology. 2017;24:31-37 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig S5	Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b	Tahiri H, Omri S, Yang C, Duhamel F, Samarani S, Ahmad A, et al. Lymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization. Sci Rep. 2016;6:37391 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Theisen E, Sauer J. Listeria monocytogenes-Induced Cell Death Inhibits the Generation of Cell-Mediated Immunity. Infect Immun. 2017;85: pubmed publisher
flow cytometry; mouse; fig 4B	Veinotte L, Gebremeskel S, Johnston B. CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFN? production and tumor control. Oncoimmunology. 2016;5:e1160979 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Chen S, Miyazaki M, Chandra V, Fisch K, Chang A, Murre C. Id3 Orchestrates Germinal Center B Cell Development. Mol Cell Biol. 2016;36:2543-52 pubmed publisher
flow cytometry; mouse	Knobeloch K, Wright M, Ochsenbein A, Liesenfeld O, Lohler J, Zinkernagel R, et al. Targeted inactivation of the tetraspanin CD37 impairs T-cell-dependent B-cell response under suboptimal costimulatory conditions. Mol Cell Biol. 2000;20:5363-9 pubmed
	van der Sluis T, Beyrend G, van der Gracht E, Abdelaal T, Jochems S, Belderbos R, et al. OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum. Cell Rep Med. 2023;4:100939 pubmed publisher
	Wu X, Zeng H, Xu C, Chen H, Fan L, Zhou H, et al. TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage. Front Immunol. 2021;12:766178 pubmed publisher
	Salerno F, Guislain A, Freen van Heeren J, Nicolet B, Young H, Wolkers M. Critical role of post-transcriptional regulation for IFN-γ in tumor-infiltrating T cells. Oncoimmunology. 2019;8:e1532762 pubmed publisher
	Guerrini M, Okamoto K, Komatsu N, Sawa S, Danks L, Penninger J, et al. Inhibition of the TNF Family Cytokine RANKL Prevents Autoimmune Inflammation in the Central Nervous System. Immunity. 2015;43:1174-85 pubmed publisher
	Attridge K, Kenefeck R, Wardzinski L, Qureshi O, Wang C, Manzotti C, et al. IL-21 promotes CD4 T cell responses by phosphatidylinositol 3-kinase-dependent upregulation of CD86 on B cells. J Immunol. 2014;192:2195-201 pubmed publisher
	Thomas D, Wong F, Zaccone P, Green E, Wallberg M. Protection of islet grafts through transforming growth factor-?-induced tolerogenic dendritic cells. Diabetes. 2013;62:3132-42 pubmed publisher
	Leonhardt F, Grundmann S, Behe M, Bluhm F, Dumont R, Braun F, et al. Inflammatory neovascularization during graft-versus-host disease is regulated by ?v integrin and miR-100. Blood. 2013;121:3307-18 pubmed publisher
	Fontenot J, Dooley J, Farr A, Rudensky A. Developmental regulation of Foxp3 expression during ontogeny. J Exp Med. 2005;202:901-6 pubmed

product information

Product Type :

Antibody

Product Name :

CD86 Monoclonal Antibody (GL-1)

Catalog # :

MA1-10299

Quantity :

100 ug

Price :

US 209.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 2 ug/mL, Functional Assay: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Mouse

Clone :

GL-1

Isotype :

IgG2a, kappa

Storage :

4 C, do not freeze

Description :

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Immunogen :

LPS-activated CBA/Cs mouse splenic B cells

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 2 ug/mL, Functional Assay: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Aliases :

activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2

more info or order :

Invitrogen product webpage