product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD86 Monoclonal Antibody (BU63)
catalog :
MA1-10293
quantity :
100 ug
price :
US 238.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BU63
reactivity :
baboons, human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 34
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3d
Mytych J, Romerowicz Misielak M, Koziorowski M. Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes. Toxicol In Vitro. 2017;42:1-9 pubmed publisher
  • flow cytometry; human; fig 8
Babu R, Brown A. A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages. Retrovirology. 2013;10:155 pubmed publisher
  • flow cytometry; human
St Gelais C, Coleman C, Wang J, Wu L. HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation. PLoS ONE. 2012;7:e34521 pubmed publisher
  • flow cytometry; human; tbl 3
Garbe K, Bratke K, Wagner S, Virchow J, Lommatzsch M. Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age. Hum Immunol. 2012;73:493-7 pubmed publisher
  • flow cytometry; human; fig 1
Kim J, Kim Y, Jeoung D, Choe J. Human follicular dendritic cells promote the APC capability of B cells by enhancing CD86 expression levels. Cell Immunol. 2012;273:109-14 pubmed publisher
  • flow cytometry; human; tbl 1
Jones H, Klein N, Dixon G. Human dendritic cell culture and bacterial infection. Methods Mol Biol. 2012;799:217-35 pubmed publisher
  • flow cytometry; human
Coleman C, Spearman P, Wu L. Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef. Retrovirology. 2011;8:26 pubmed publisher
  • flow cytometry; human; fig 5
Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger G, Stuhlmeier K, et al. The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells. J Immunol. 2009;183:4440-8 pubmed publisher
  • flow cytometry; human; fig 3
Kuhne M, Erben U, Schulze Tanzil G, Köhler D, Wu P, Richter F, et al. HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis. Arthritis Rheum. 2009;60:1635-46 pubmed publisher
  • flow cytometry; human; fig 3
Stephens T, Nikoopour E, Rider B, Leon Ponte M, Chau T, Mikolajczak S, et al. Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E. J Immunol. 2008;181:6859-71 pubmed
  • flow cytometry; human; fig 3
Jacobi A, Reiter K, Mackay M, Aranow C, Hiepe F, Radbruch A, et al. Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95. Arthritis Rheum. 2008;58:1762-73 pubmed publisher
  • flow cytometry; human; tbl 1
Kolar G, Mehta D, Pelayo R, Capra J. A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007;109:2545-52 pubmed
  • flow cytometry; human; tbl 2
  • immunocytochemistry; human; fig 2h
Sandilands G, McCrae J, Hill K, Perry M, Baxter D. Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils. Immunology. 2006;119:562-71 pubmed
  • flow cytometry; human
Summers K, Marleau A, Mahon J, McManus R, Hramiak I, Singh B. Reduced IFN-alpha secretion by blood dendritic cells in human diabetes. Clin Immunol. 2006;121:81-9 pubmed
  • flow cytometry; human; fig 1
Reis E, Barbuto J, Isaac L. Human monocyte-derived dendritic cells are a source of several complement proteins. Inflamm Res. 2006;55:179-84 pubmed
  • flow cytometry; human
Lopez Santalla M, Valeri A, Perez Blas M, Aguilera Montilla N, Gutierrez A, Lasa I, et al. Expression of CD45 and proliferative response to CD3 as suitable classification markers of patients with gastric adenocarcinoma. Cancer Immunol Immunother. 2006;55:744-8 pubmed
  • flow cytometry; human; fig 4
Kirchberger S, Majdic O, Steinberger P, Bluml S, Pfistershammer K, Zlabinger G, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005;175:1145-52 pubmed
  • flow cytometry; human; fig 1
Bluml S, Kirchberger S, Bochkov V, Kronke G, Stuhlmeier K, Majdic O, et al. Oxidized phospholipids negatively regulate dendritic cell maturation induced by TLRs and CD40. J Immunol. 2005;175:501-8 pubmed
  • flow cytometry; human; 3.3 ug/ml
McIlroy D, Tanguy Royer S, Le Meur N, Guisle I, Royer P, Léger J, et al. Profiling dendritic cell maturation with dedicated microarrays. J Leukoc Biol. 2005;78:794-803 pubmed
  • flow cytometry; human
Game D, Rogers N, Lechler R. Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells. Am J Transplant. 2005;5:1614-25 pubmed
  • flow cytometry; human; tbl 1
Lozza L, Lilleri D, Percivalle E, Fornara C, Comolli G, Revello M, et al. Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV-infected immature dendritic cells. Eur J Immunol. 2005;35:1795-804 pubmed
  • flow cytometry; human
Aguilera Montilla N, Perez Blas M, Valeri A, Lopez Santalla M, Rodríguez Juan C, Mencia A, et al. Higher proliferative capacity of T lymphocytes from patients with Crohn disease than from ulcerative colitis is disclosed by use of Herpesvirus saimiri-transformed T-cell lines. Scand J Gastroenterol. 2004;39:1236-42 pubmed
  • flow cytometry; human; fig 1
Kudela P, Paukner S, Mayr U, Cholujova D, Schwarczova Z, Sedlak J, et al. Bacterial ghosts as novel efficient targeting vehicles for DNA delivery to the human monocyte-derived dendritic cells. J Immunother. 2005;28:136-43 pubmed
  • flow cytometry; human; fig 6
Sandilands G, Ahmed Z, Perry N, Davison M, Lupton A, Young B. Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation. Immunology. 2005;114:354-68 pubmed
  • flow cytometry; human; fig 4
Pfistershammer K, Majdic O, Stockl J, Zlabinger G, Kirchberger S, Steinberger P, et al. CD63 as an activation-linked T cell costimulatory element. J Immunol. 2004;173:6000-8 pubmed
  • flow cytometry; human
Zingoni A, Sornasse T, Cocks B, Tanaka Y, Santoni A, Lanier L. Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions. J Immunol. 2004;173:3716-24 pubmed
  • flow cytometry; baboons
Frost P, Hubbard G, Dammann M, Snider C, Moore C, Hodara V, et al. White monkey syndrome in infant baboons (Papio species). J Med Primatol. 2004;33:197-213 pubmed
  • flow cytometry; human
Valeri A, Perez Blas M, Gutierrez A, Lopez Santalla M, Aguilera N, Rodríguez Juan C, et al. Intrinsic defects explain altered proliferative responses of T lymphocytes and HVS-derived T-cell lines in gastric adenocarcinoma. Cancer Immunol Immunother. 2003;52:708-14 pubmed
  • ELISA; human; fig 6
Selenko Gebauer N, Majdic O, Szekeres A, Höfler G, Guthann E, Korthauer U, et al. B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy. J Immunol. 2003;170:3637-44 pubmed
  • flow cytometry; human
McDowell M, Marovich M, Lira R, Braun M, Sacks D. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect Immun. 2002;70:3994-4001 pubmed
  • flow cytometry; human; fig 3
Berg L, James M, Alvarez Iglesias M, Glennie S, Lechler R, Marelli Berg F. Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium. J Immunol. 2002;168:3227-34 pubmed
  • flow cytometry; human
Steinberger P, Szekeres A, Wille S, Stockl J, Selenko N, Prager E, et al. Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning. J Leukoc Biol. 2002;71:133-40 pubmed
Hou T, Li S, Zhang G, Li Y. High-fluence low-power laser irradiation promotes odontogenesis and inflammation resolution in periodontitis by enhancing stem cell proliferation and differentiation. Int J Mol Med. 2018;42:2107-2119 pubmed publisher
Morbach H, Wiegering V, Richl P, Schwarz T, Suffa N, Eichhorn E, et al. Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis. Arthritis Rheum. 2011;63:3458-66 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD86 Monoclonal Antibody (BU63)
Catalog # :
MA1-10293
Quantity :
100 ug
Price :
US 238.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 5 ug/ml, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent
Species :
Human
Clone :
BU63
Isotype :
IgG1
Storage :
4 C, do not freeze
Description :
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Immunogen :
B-lymphoblastoid cell line ARH 77
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 ug/ml, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent
Aliases :
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA