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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD16 Monoclonal Antibody (3G8)
catalog :
MA1-10112
quantity :
100 ug
price :
US 238.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3G8
reactivity :
human, mouse, rhesus macaque
application :
ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 46
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; tbl 4
Fromm J, Tagliente D, Shaver A, Neppalli V, Craig F. Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014. Cytometry B Clin Cytom. 2015;88:413-24 pubmed publisher
  • flow cytometry; human
Stacchini A, Pacchioni D, Demurtas A, Aliberti S, Cassenti A, Isolato G, et al. Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas. Cytometry B Clin Cytom. 2015;88:320-9 pubmed publisher
  • flow cytometry; human
Kobie J, Treanor J, Ritchlin C. Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody. Immunol Invest. 2014;43:606-15 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 3
Cepeda M, Salas M, Folwarczny J, Leandro A, Hodara V, De La Garza M, et al. Establishment of a neonatal rhesus macaque model to study Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2013;93 Suppl:S51-9 pubmed publisher
  • flow cytometry; human; fig 1
Kumpel B, Hazell M, Guest A, Dixey J, Mushens R, Bishop D, et al. Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis. Transfusion. 2014;54:1305-16 pubmed publisher
  • flow cytometry; human; tbl 1
Stacchini A, Aliberti S, Pacchioni D, Demurtas A, Isolato G, Gazzera C, et al. Flow cytometry significantly improves the diagnostic value of fine needle aspiration cytology of lymphoproliferative lesions of salivary glands. Cytopathology. 2014;25:231-40 pubmed publisher
  • flow cytometry; human; fig 2
Luiza Silva M, Campi Azevedo A, Batista M, Martins M, Avelar R, da Silveira Lemos D, et al. Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody. J Infect Dis. 2011;204:873-83 pubmed publisher
  • flow cytometry; mouse
Qian B, Li J, Zhang H, Kitamura T, Zhang J, Campion L, et al. CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis. Nature. 2011;475:222-5 pubmed publisher
  • flow cytometry; human; fig 1, 2, 3
Bratke K, Klein C, Kuepper M, Lommatzsch M, Virchow J. Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. Allergy. 2011;66:386-95 pubmed publisher
  • flow cytometry; human
Schrauf C, Kirchberger S, Majdic O, Seyerl M, Zlabinger G, Stuhlmeier K, et al. The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells. J Immunol. 2009;183:4440-8 pubmed publisher
  • flow cytometry; human; fig 3
Tanaka M, Krutzik S, Sieling P, Lee D, Rea T, Modlin R. Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses. J Immunol. 2009;183:2349-55 pubmed publisher
  • flow cytometry; rhesus macaque
Hokey D, Yan J, Hirao L, Dai A, Boyer J, Jure Kunkel M, et al. CLTA-4 blockade in vivo promotes the generation of short-lived effector CD8 T cells and a more persistent central memory CD4 T cell response. J Med Primatol. 2008;37 Suppl 2:62-8 pubmed publisher
  • flow cytometry; human
Giannelli S, Taddeo A, Presicce P, Villa M, Della Bella S. A six-color flow cytometric assay for the analysis of peripheral blood dendritic cells. Cytometry B Clin Cytom. 2008;74:349-55 pubmed publisher
  • flow cytometry; human; fig 1
Sathler Avelar R, Vitelli Avelar D, Massara R, de Lana M, Pinto Dias J, Teixeira Carvalho A, et al. Etiological treatment during early chronic indeterminate Chagas disease incites an activated status on innate and adaptive immunity associated with a type 1-modulated cytokine pattern. Microbes Infect. 2008;10:103-13 pubmed publisher
  • flow cytometry; human; fig 4
Lee D, Sieling P, Ochoa M, Krutzik S, Guo B, Hernandez M, et al. LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007;179:8128-36 pubmed
  • flow cytometry; human
Summers K, Marleau A, Mahon J, McManus R, Hramiak I, Singh B. Reduced IFN-alpha secretion by blood dendritic cells in human diabetes. Clin Immunol. 2006;121:81-9 pubmed
  • flow cytometry; human
Hirano N, Butler M, Xia Z, Ansén S, von Bergwelt Baildon M, Neuberg D, et al. Engagement of CD83 ligand induces prolonged expansion of CD8+ T cells and preferential enrichment for antigen specificity. Blood. 2006;107:1528-36 pubmed
  • flow cytometry; human
Siliciano J, Siliciano R. Enhanced culture assay for detection and quantitation of latently infected, resting CD4+ T-cells carrying replication-competent virus in HIV-1-infected individuals. Methods Mol Biol. 2005;304:3-15 pubmed
  • flow cytometry; human
Kirchberger S, Majdic O, Steinberger P, Bluml S, Pfistershammer K, Zlabinger G, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005;175:1145-52 pubmed
  • flow cytometry; human
Mittag A, Lenz D, Gerstner A, Sack U, Steinbrecher M, Koksch M, et al. Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets. Cytometry A. 2005;65:103-15 pubmed
  • flow cytometry; human; fig 2
Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, et al. Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy. J Immunol Methods. 2004;295:67-78 pubmed
  • flow cytometry; human
Pfistershammer K, Majdic O, Stockl J, Zlabinger G, Kirchberger S, Steinberger P, et al. CD63 as an activation-linked T cell costimulatory element. J Immunol. 2004;173:6000-8 pubmed
  • flow cytometry; human; fig 2C
  • immunohistochemistry; human; fig 2C
Eriksson M, Meadows S, Wira C, Sentman C. Unique phenotype of human uterine NK cells and their regulation by endogenous TGF-beta. J Leukoc Biol. 2004;76:667-75 pubmed
  • flow cytometry; human; fig 1
Steinberger P, Majdic O, Derdak S, Pfistershammer K, Kirchberger S, Klauser C, et al. Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains. J Immunol. 2004;172:2352-9 pubmed
  • flow cytometry; human
Suskind D, Muench M. Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells. J Hepatol. 2004;40:261-8 pubmed
  • flow cytometry; human
Rahimi K, Maerz H, Zotz R, Tarnok A. Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease. Cytometry B Clin Cytom. 2003;53:63-9 pubmed
  • ELISA; human; fig 6
Selenko Gebauer N, Majdic O, Szekeres A, Höfler G, Guthann E, Korthauer U, et al. B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy. J Immunol. 2003;170:3637-44 pubmed
  • flow cytometry; human
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed
  • flow cytometry; human
Di Bona E, Sartori R, Zambello R, Guercini N, Madeo D, Rodeghiero F. Prognostic significance of CD56 antigen expression in acute myeloid leukemia. Haematologica. 2002;87:250-6 pubmed
  • flow cytometry; human; 2 ug/ml
McIlroy D, Troadec C, Grassi F, Samri A, Barrou B, Autran B, et al. Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors. Blood. 2001;97:3470-7 pubmed
  • flow cytometry; human; fig 2
Sharron M, Pohlmann S, Price K, Lolis E, Tsang M, Kirchhoff F, et al. Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes. Blood. 2000;96:41-9 pubmed
  • flow cytometry; human
Gopinath R, Hanna L, Kumaraswami V, Perumal V, Kavitha V, Vijayasekaran V, et al. Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis. Infect Immun. 2000;68:93-9 pubmed
  • flow cytometry; human
Lee B, Sharron M, Montaner L, Weissman D, Doms R. Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages. Proc Natl Acad Sci U S A. 1999;96:5215-20 pubmed
  • flow cytometry; human; fig 1
  • immunoprecipitation; human; fig 2
Ravetch J, Perussia B. Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J Exp Med. 1989;170:481-97 pubmed
  • immunoprecipitation; human; fig 4
Peltz G, Grundy H, Lebo R, Yssel H, Barsh G, Moore K. Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG. Proc Natl Acad Sci U S A. 1989;86:1013-7 pubmed
Bauman J, Ohr J, Gooding W, Ferris R, Duvvuri U, Kim S, et al. Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer. Cancers (Basel). 2020;12: pubmed publisher
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Hayashi T, Nakachi K, et al. Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors. J Immunol. 2017;199:2777-2793 pubmed publisher
Cheeseman H, Olejniczak N, Rogers P, Evans A, King D, Ziprin P, et al. Broadly Neutralizing Antibodies Display Potential for Prevention of HIV-1 Infection of Mucosal Tissue Superior to That of Nonneutralizing Antibodies. J Virol. 2017;91: pubmed publisher
Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
Jordan N, Bardia A, Wittner B, Benes C, Ligorio M, Zheng Y, et al. HER2 expression identifies dynamic functional states within circulating breast cancer cells. Nature. 2016;537:102-106 pubmed publisher
Williams D, Engle E, Shirk E, Queen S, Gama L, Mankowski J, et al. Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol. 2016;186:2068-2087 pubmed publisher
Clémenceau B, Valsesia Wittmann S, Jallas A, Vivien R, Rousseau R, Marabelle A, et al. In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer. J Immunol Res. 2015;2015:482089 pubmed publisher
Rosario M, Liu B, Kong L, Collins L, Schneider S, Chen X, et al. The IL-15-Based ALT-803 Complex Enhances FcγRIIIa-Triggered NK Cell Responses and In Vivo Clearance of B Cell Lymphomas. Clin Cancer Res. 2016;22:596-608 pubmed publisher
Kinder M, Greenplate A, Strohl W, Jordan R, Brezski R. An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions. MAbs. 2015;7:494-504 pubmed publisher
Algra S, Groeneveld K, Schadenberg A, Haas F, Evens F, Meerding J, et al. Cerebral ischemia initiates an immediate innate immune response in neonates during cardiac surgery. J Neuroinflammation. 2013;10:24 pubmed publisher
Berard F, Blanco P, Davoust J, Neidhart Berard E, Nouri Shirazi M, Taquet N, et al. Cross-priming of naive CD8 T cells against melanoma antigens using dendritic cells loaded with killed allogeneic melanoma cells. J Exp Med. 2000;192:1535-44 pubmed
product information
Product Type :
Antibody
Product Name :
CD16 Monoclonal Antibody (3G8)
Catalog # :
MA1-10112
Quantity :
100 ug
Price :
US 238.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Non-human primate
Applications :
Flow Cytometry: 6 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent
Species :
Human, Non-human primate
Clone :
3G8
Isotype :
IgG1, kappa
Storage :
4 C, do not freeze
Description :
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
Immunogen :
Human neutrophils
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 6 ug/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent
Aliases :
4833442P21Rik; CD16; CD16-2; CD16A; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor IIIb; Fc gamma RIIIa; Fc gammaRIV; Fc receptor, IgG, low affinity III; Fc receptor, IgG, low affinity IV; Fc receptor-like 3; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; fc-gamma RIIIb; fc-gamma RIII-beta; Fc-gamma-RIIb; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIII a.1; FcgammaRIII a.2; FcgammaRIII a.3; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FCGR3; Fcgr3a; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FcR-10; FcRII-b; FcRII-c; FcRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IgG Fc gamma receptor III; igG Fc receptor III; IgG Fc receptor III-1; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; LOC100911825; Low affinity immunoglobulin gamma Fc region receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; low affinity immunoglobulin gamma Fc region receptor III-like; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA