This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Ubiquitin Monoclonal Antibody (Ubi-1)
catalog :
MA1-10035
quantity :
100µL
price :
US 375.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Ubi-1
reactivity :
fission yeast, human, mouse, rat, chicken, cow, pig, fruit fly, rabbit, equine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 25
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; fig 1d
  • western blot; mouse; fig 1e
Leal-Lasarte M, Franco J, Labrador-Garrido A, Pozo D, Roodveldt C. Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia.. FASEB J. 2017;0: pubmed publisher
  • western blot; rat; 1:1000; fig 5
Grindheim A, Hollås H, Raddum A, Saraste J, Vedeler A. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2. J Cell Sci. 2016;129:314-28 pubmed publisher
  • western blot; human; 1:1000; fig 6b
Koh H, Scruggs A, Huang S. Transforming Growth Factor-β1 Increases DNA Methyltransferase 1 and 3a Expression through Distinct Post-transcriptional Mechanisms in Lung Fibroblasts. J Biol Chem. 2016;291:19287-98 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
Peters O, Shelkovnikova T, Highley J, Cooper-Knock J, Hortobagyi T, Troakes C, et al. Gamma-synuclein pathology in amyotrophic lateral sclerosis. Ann Clin Transl Neurol. 2015;2:29-37 pubmed publisher
  • western blot; fruit fly; 1:500
Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun. 2015;6:7520 pubmed publisher
  • immunohistochemistry - paraffin section; human
Guarino M, Ballabio G, Rubino B, Nebuloni M, Tosoni A. Soft tissue sacrococcygeal chordoma with intracytoplasmic filamentous inclusions. Pathol Res Pract. 2005;201:699-704 pubmed
  • western blot; human
Wang Y, Bruce A, Tu C, Ma K, Zeng L, Zheng P, et al. Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes. J Cell Sci. 2011;124:3568-80 pubmed publisher
  • western blot; human
Nakamura N, Hirose S. Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane. Mol Biol Cell. 2008;19:1903-11 pubmed publisher
  • immunohistochemistry - paraffin section; rabbit; 1:1500; fig 3
Huang Y, Herman M, Liu J, Katsetos C, Wills M, Savory J. Neurofibrillary lesions in experimental aluminum-induced encephalopathy and Alzheimer's disease share immunoreactivity for amyloid precursor protein, A beta, alpha 1-antichymotrypsin and ubiquitin-protein conjugates. Brain Res. 1997;771:213-20 pubmed
  • immunohistochemistry; human; 1:3
Lobrinus J, Schorderet D, Payot M, Jeanrenaud X, Bottani A, Superti-Furga A, et al. Morphological, clinical and genetic aspects in a family with a novel LAMP-2 gene mutation (Danon disease). Neuromuscul Disord. 2005;15:293-8 pubmed
  • immunocytochemistry; mouse; 2.5 ug/ml
Koga H, Martinez-Vicente M, Macian F, Verkhusha V, Cuervo A. A photoconvertible fluorescent reporter to track chaperone-mediated autophagy. Nat Commun. 2011;2:386 pubmed publisher
  • western blot; human; 1:1000
Mullally J, Moos P, Edes K, Fitzpatrick F. Cyclopentenone prostaglandins of the J series inhibit the ubiquitin isopeptidase activity of the proteasome pathway. J Biol Chem. 2001;276:30366-73 pubmed
  • western blot; human; 1 ug/ml; fig 2
Mori T, Li Y, Hata H, Kochi H. NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein. FEBS Lett. 2004;557:209-14 pubmed
  • western blot; human
Sternsdorf T, Puccetti E, Jensen K, Hoelzer D, Will H, Ottmann O, et al. PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia. Mol Cell Biol. 1999;19:5170-8 pubmed
  • western blot; fission yeast; fig 4
  • western blot; human; fig 4
Pebernard S, Perry J, Tainer J, Boddy M. Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Mol Biol Cell. 2008;19:4099-109 pubmed publisher
  • immunocytochemistry; human
Pogacic V, Dragon F, Filipowicz W. Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10. Mol Cell Biol. 2000;20:9028-40 pubmed
  • western blot; human; 1:1000; fig 4
Shintani T, Yamazaki F, Katoh T, Umekawa M, Matahira Y, Hori S, et al. Glucosamine induces autophagy via an mTOR-independent pathway. Biochem Biophys Res Commun. 2010;391:1775-9 pubmed publisher
  • immunocytochemistry; human
Zeng L, Wang Y, Baba O, Zheng P, Liu Y, Liu Y. Laforin is required for the functional activation of malin in endoplasmic reticulum stress resistance in neuronal cells. FEBS J. 2012;279:2467-78 pubmed publisher
  • western blot; human
van Bemmelen M, Rougier J, Gavillet B, Apothéloz F, Daidié D, Tateyama M, et al. Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination. Circ Res. 2004;95:284-91 pubmed
  • immunohistochemistry - paraffin section; human; 1:1000; tbl 2
Arnold S, Han L, Clark C, Grossman M, Trojanowski J. Quantitative neurohistological features of frontotemporal degeneration. Neurobiol Aging. 2000;21:913-9 pubmed
  • immunohistochemistry - frozen section; rat; 1:200; fig 3
Tan Z, Tu W, Schreiber S. Downregulation of free ubiquitin: a novel mechanism of p53 stabilization and neuronal cell death. Brain Res Mol Brain Res. 2001;91:179-88 pubmed
  • western blot; mouse; fig 8
Van der Put E, Frasca D, King A, Blomberg B, Riley R. Decreased E47 in senescent B cell precursors is stage specific and regulated posttranslationally by protein turnover. J Immunol. 2004;173:818-27 pubmed
  • immunohistochemistry - frozen section; rat
Tan Z, Qu W, Tu W, Liu W, Baudry M, Schreiber S. p53 accumulation due to down-regulation of ubiquitin: relevance for neuronal apoptosis. Cell Death Differ. 2000;7:675-81 pubmed
  • western blot; mouse; fig 4A
King A, Van der Put E, Blomberg B, Riley R. Accelerated Notch-dependent degradation of E47 proteins in aged B cell precursors is associated with increased ERK MAPK activation. J Immunol. 2007;178:3521-9 pubmed
Willuweit A, Velden J, Godemann R, Manook A, Jetzek F, Tintrup H, et al. Early-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease. PLoS ONE. 2009;4:e7931 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Ubiquitin Monoclonal Antibody (Ubi-1)
Catalog # :
MA1-10035
Quantity :
100µL
Price :
US 375.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Bovine, C. elegans, Chicken, Drosophila, Equine, Human, Mouse, Porcine, Rat
Applications :
Immunocytochemistry: 1:5000, Immunofluorescence: 1:5000, Immunohistochemistry (Frozen): 1:5000, Immunohistochemistry (Paraffin): 1:5000, Western Blot: 1:1000
Species :
Bovine, C. elegans, Chicken, Drosophila, Equine, Human, Mouse, Porcine, Rat
Clone :
Ubi-1
Isotype :
IgG1, kappa
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
Ubiquitin is a highly conserved globular 76 amino acid protein of about 8.5 kDa molecular weight. It has an important role in the targeting of proteins for proteolytic degradation. Proteins to be degraded are covalantly coupled to the C-terminus of ubiquitin by means of ubiquitin ligases. The ubiquitin itself is frequently also ubiquitinated, producing a polyubiquitin chain.
Immunogen :
Purified ubiquitin conjugated with glutaroldehyde to KLH
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:5000, Immunofluorescence: 1:5000, Immunohistochemistry (Frozen): 1:5000, Immunohistochemistry (Paraffin): 1:5000, Western Blot: 1:1000
Aliases :
60S ribosomal protein L40, HUBCEP52, RPL40, Ubiquitin 52 amino acid fusion protein, Ubiquitin A52 residue ribosomal protein fusion product 1, Ubiquiti
PubMed References :
10373566, 10889512, 11074001, 11124442, 11390388, 11457508, 14741369, 15217910, 15240668, 15792868, 16325512, 17339447, 18287522, 18667531, 20045674, 21750540, 22045735, 22578008, 26138142, 26644180, 9401741
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
www.thermofisher.com
800-678-5599
headquarters: USA