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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
eIF4E Monoclonal Antibody (5D11)
catalog :
MA1-089
quantity :
100 µg
price :
US 480.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5D11
reactivity :
human, mouse
application :
western blot, immunocytochemistry, immunoprecipitation, RNA immunoprecipitation
more info or order :
Invitrogen product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; mouse; loading ...; fig 2b
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4b
  • western blot; mouse; loading ...; fig 2b
Hu J, Sun F, Handel M. Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. Biol Reprod. 2018;98:102-114 pubmed publisher
Keane L, Antignano I, Riechers S, Zollinger R, Dumas A, Offermann N, et al. mTOR-dependent translation amplifies microglia priming in aging mice. J Clin Invest. 2021;131: pubmed publisher
image
image 1 :
Invitrogen MA1-089 image 1
Immunoprecipitation of eIF4E was performed on HeLa cell lysates. The antigen:antibody complex was formed by incubating 500µg whole cell lysate with 3µg of mouse monoclonal antibody recognizing eIF4E (Product # MA1-089) overnight on a rocking platform at 4°C. The immune-complex was captured on 50µl Protein A/G Plus Agarose (Product #20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF4E (Product # MA1-089) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBST and probed with Pierce Clean Blot (Product #21230) at a dilution of 1:1,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
image 2 :
Invitrogen MA1-089 image 2
Western blot analysis of eIF4E was performed by loading 80µg whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF4E (Product #MA1-089) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34080).
product information
Product Type :
Antibody
Product Name :
eIF4E Monoclonal Antibody (5D11)
Catalog # :
MA1-089
Quantity :
100 µg
Price :
US 480.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
Immunocytochemistry: 1:100-1:500, Immunoprecipitation: 3-5 µg, RNA Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Species :
Human, Mouse
Clone :
5D11
Isotype :
IgG1
Storage :
-20°C
Description :
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
Immunogen :
Full length human eIF4E produced in HEK293T cells
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:100-1:500, Immunoprecipitation: 3-5 µg, RNA Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Aliases :
AUTS19; CBP; EG668879; EIF4E; EIF-4E; EIF4E1; EIF4EL1; Eif4e-ps; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E-like protein; eukaryotic translation initiation factor 4-like protein; If4e; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; translation initiation factor eIF-4E
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA