product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CREB Monoclonal Antibody (LB9)
catalog :
MA1-083
quantity :
100 µg
price :
US 425.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LB9
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 4
Published Application/Species/Sample/DilutionReference
  • western blot; rat; loading ...; fig 5b
Bazzari F, Abdallah D, El Abhar H. Chenodeoxycholic Acid Ameliorates AlCl3-Induced Alzheimer's Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats. Molecules. 2019;24: pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig s8
Amal H, Gong G, Gjoneska E, Lewis S, Wishnok J, Tsai L, et al. S-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy. Transl Psychiatry. 2019;9:44 pubmed publisher
Wang L, Nie Q, Gao M, Yang L, Xiang J, Xiao Y, et al. The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression. Aging (Albany NY). 2020;12:13594-13617 pubmed publisher
Elezaby A, Sverdlov A, Tu V, Soni K, Luptak I, Qin F, et al. Mitochondrial remodeling in mice with cardiomyocyte-specific lipid overload. J Mol Cell Cardiol. 2015;79:275-83 pubmed publisher
image
image 1 :
Invitrogen MA1-083 image 1
Immunofluorescent analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB (Product # MA1-083), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
image 2 :
Invitrogen MA1-083 image 2
Immunohistochemistry was performed on cancer biopsies of deparaffinized human bladder and rectal tissue. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CREB (Product # MA1-083) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB. Images are displayed at 40X magnification. Results demonstrate strong nuclear localization of CREB.
image 3 :
Invitrogen MA1-083 image 3
Western Blot analysis of CREB in HepG2 cells using a mouse monoclonal antibody recognizing CREB (Product #MA1-083).
product information
Product Type :
Antibody
Product Name :
CREB Monoclonal Antibody (LB9)
Catalog # :
MA1-083
Quantity :
100 µg
Price :
US 425.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Human, Mouse, Non-human primate, Rat
Applications :
ChIP assay: 2.5 µg/1x10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5 µg/mL, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500
Species :
Human, Mouse, Non-human primate, Rat
Clone :
LB9
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.
Immunogen :
Recombinant human CREB-A protein.
Format :
Liquid
Applications w/Dilutions :
ChIP assay: 2.5 µg/1x10^6 cells, ELISA: 0.1-1.0 µg/mL, Immunocytochemistry: 5 µg/mL, Immunohistochemistry: 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500
Aliases :
2310001E10Rik; 3526402H21Rik; active transcription factor CREB; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; Creb-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA