Influenza A M2 Monoclonal Antibody (14C2) | Invitrogen MA1-082 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Influenza A M2 Monoclonal Antibody (14C2)

catalog :

MA1-082

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

14C2

reactivity :

Alphainfluenzavirus

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; Alphainfluenzavirus	Fournier G, Chiang C, Munier S, Tomoiu A, Demeret C, Vidalain P, et al. Recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase to control Viral mRNA splicing. PLoS Pathog. 2014;10:e1004164 pubmed publisher
flow cytometry; Alphainfluenzavirus	Andersson A, Hakansson K, Jensen B, Christensen D, Andersen P, Thomsen A, et al. Increased immunogenicity and protective efficacy of influenza M2e fused to a tetramerizing protein. PLoS ONE. 2012;7:e46395 pubmed publisher
western blot; Alphainfluenzavirus; 1:1000	Ivanovic T, Rozendaal R, Floyd D, Popović M, van Oijen A, Harrison S. Kinetics of proton transport into influenza virions by the viral M2 channel. PLoS ONE. 2012;7:e31566 pubmed publisher
flow cytometry; Alphainfluenzavirus immunocytochemistry; Alphainfluenzavirus western blot; Alphainfluenzavirus	Ma H, Kien F, Manière M, Zhang Y, Lagarde N, Tse K, et al. Human annexin A6 interacts with influenza a virus protein M2 and negatively modulates infection. J Virol. 2012;86:1789-801 pubmed publisher
western blot; Alphainfluenzavirus	Ozawa T, Jin A, Tajiri K, Takemoto M, Okuda T, Shiraki K, et al. Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus. Antiviral Res. 2011;91:283-7 pubmed publisher
western blot; Alphainfluenzavirus	Gannage M, Dormann D, Albrecht R, Dengjel J, Torossi T, Rämer P, et al. Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes. Cell Host Microbe. 2009;6:367-80 pubmed publisher
	Wang Y, Deng L, Gonzalez G, Luthra L, Dong C, Ma Y, et al. Double-Layered M2e-NA Protein Nanoparticle Immunization Induces Broad Cross-Protection against Different Influenza Viruses in Mice. Adv Healthc Mater. 2020;9:e1901176 pubmed publisher
	Deng L, Mohan T, Chang T, Gonzalez G, Wang Y, Kwon Y, et al. Double-layered protein nanoparticles induce broad protection against divergent influenza A viruses. Nat Commun. 2018;9:359 pubmed publisher
	Kuss Duerkop S, Wang J, Mena I, White K, Metreveli G, Sakthivel R, et al. Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication. PLoS Pathog. 2017;13:e1006635 pubmed publisher
	Jin H, Leser G, Zhang J, Lamb R. Influenza virus hemagglutinin and neuraminidase cytoplasmic tails control particle shape. EMBO J. 1997;16:1236-47 pubmed
	Hughey P, Roberts P, Holsinger L, Zebedee S, Lamb R, Compans R. Effects of antibody to the influenza A virus M2 protein on M2 surface expression and virus assembly. Virology. 1995;212:411-21 pubmed
	Zebedee S, Lamb R. Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions. J Virol. 1988;62:2762-72 pubmed
	Holsinger L, Lamb R. Influenza virus M2 integral membrane protein is a homotetramer stabilized by formation of disulfide bonds. Virology. 1991;183:32-43 pubmed

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product information

Product Type :

Antibody

Product Name :

Influenza A M2 Monoclonal Antibody (14C2)

Catalog # :

MA1-082

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Virus

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunoprecipitation: 10 µg/mL, Western Blot: 1 µg/mL

Species :

Virus

Clone :

14C2

Isotype :

IgG1, kappa

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Immunogen :

M2 protein from A/WSN/33-infected CV1 cell lysate.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunoprecipitation: 10 µg/mL, Western Blot: 1 µg/mL

Aliases :

Influenza M2

more info or order :

Invitrogen product webpage