NUP62 Monoclonal Antibody (RL1) | Invitrogen MA1-071 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NUP62 Monoclonal Antibody (RL1)

catalog :

MA1-071

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RL1

reactivity :

marine lamprey, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; marine lamprey; 1:100; fig s2	Timoshevskiy V, Herdy J, Keinath M, Smith J. Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus). PLoS Genet. 2016;12:e1006103 pubmed publisher
immunocytochemistry; mouse; 1:750	Sinclair P, Bian Q, Plutz M, Heard E, Belmont A. Dynamic plasticity of large-scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol. 2010;190:761-76 pubmed publisher
	Forsythe M, Love D, Lazarus B, Kim E, Prinz W, Ashwell G, et al. Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer. Proc Natl Acad Sci U S A. 2006;103:11952-7 pubmed
	Castagnet P, Mavlyutov T, Cai Y, Zhong F, Ferreira P. RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons. Hum Mol Genet. 2003;12:1847-63 pubmed
	Brownawell A, Macara I. Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins. J Cell Biol. 2002;156:53-64 pubmed
	Bobinnec Y, Fukuda M, Nishida E. Identification and characterization of Caenorhabditis elegans gamma-tubulin in dividing cells and differentiated tissues. J Cell Sci. 2000;113 Pt 21:3747-59 pubmed
	Kehlenbach R, Dickmanns A, Kehlenbach A, Guan T, Gerace L. A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export. J Cell Biol. 1999;145:645-57 pubmed
	Yang L, Guan T, Gerace L. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol. 1997;139:1077-87 pubmed
	Byrd D, Sweet D, Pante N, Konstantinov K, Guan T, Saphire A, et al. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex. J Cell Biol. 1994;127:1515-26 pubmed
	Gorsch L, Dockendorff T, Cole C. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol. 1995;129:939-55 pubmed
	Holt G, Snow C, Senior A, Haltiwanger R, Gerace L, Hart G. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol. 1987;104:1157-64 pubmed
	Featherstone C, Darby M, Gerace L. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. J Cell Biol. 1988;107:1289-97 pubmed
	Snow C, Senior A, Gerace L. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol. 1987;104:1143-56 pubmed
	Sterne Marr R, Blevitt J, Gerace L. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol. 1992;116:271-80 pubmed
	Greber U, Gerace L. Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein. J Cell Biol. 1992;116:15-30 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein (MA1-071) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein (MA1-071) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein (MA1-071) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

NUP62 Monoclonal Antibody (RL1)

Catalog # :

MA1-071

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

PEG precipitation

Host :

Mouse

Reactivity :

Amphibian, Rat, Yeast

Applications :

Immunohistochemistry: 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Species :

Amphibian, Rat, Yeast

Clone :

RL1

Isotype :

IgM

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

SDS3 is a subunit of the histone deacetylase.

Immunogen :

Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: 1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000

Aliases :

62 kDa nucleoporin; AA589433; AI426861; AI790512; AU045898; D7Ertd649e; DKFZp547L134; FLJ20822; FLJ43869; IBSN; MGC841; Np62; nuclear pore complex 1; nuclear pore glycoprotein 62; nuclear pore glycoprotein p62; nucleoporin 62; nucleoporin 62kD; nucleoporin 62kDa; nucleoporin Nup62; nucleoporin p62; Nup62; Nupc1; p62; SNDI

more info or order :

Invitrogen product webpage