Dynein Monoclonal Antibody (74.1) | Invitrogen MA1-070 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Dynein Monoclonal Antibody (74.1)

catalog :

MA1-070

quantity :

100 µg

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

74.1

reactivity :

human, mouse, rat, bovine

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:250; fig 6	NÃ¼ÃŸe J, Mirastschijski U, Waespy M, Oetjen J, Brandes N, Rebello O, et al. Two new isoforms of the human hepatoma-derived growth factor interact with components of the cytoskeleton. Biol Chem. 2016;397:417-36 pubmed publisher
	Kelkar S, Pfister K, Crystal R, Leopold P. Cytoplasmic dynein mediates adenovirus binding to microtubules. J Virol. 2004;78:10122-32 pubmed
	Varadi A, Johnson Cadwell L, Cirulli V, Yoon Y, Allan V, Rutter G. Cytoplasmic dynein regulates the subcellular distribution of mitochondria by controlling the recruitment of the fission factor dynamin-related protein-1. J Cell Sci. 2004;117:4389-400 pubmed
	Pfister K, Salata M, Dillman J, Torre E, Lye R. Identification and developmental regulation of a neuron-specific subunit of cytoplasmic dynein. Mol Biol Cell. 1996;7:331-43 pubmed
	Pfister K, Salata M, Dillman J, Vaughan K, Vallee R, Torre E, et al. Differential expression and phosphorylation of the 74-kDa intermediate chains of cytoplasmic dynein in cultured neurons and glia. J Biol Chem. 1996;271:1687-94 pubmed
	Dillman J, Dabney L, Pfister K. Cytoplasmic dynein is associated with slow axonal transport. Proc Natl Acad Sci U S A. 1996;93:141-4 pubmed
	Dillman J, Pfister K. Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles. J Cell Biol. 1994;127:1671-81 pubmed
	Paschal B, Mikami A, Pfister K, Vallee R. Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function. J Cell Biol. 1992;118:1133-43 pubmed

image

image 1 :

image 2 :

Immunofluorescent analysis of Dynein in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody (Product # MA1-070) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

Dynein Monoclonal Antibody (74.1)

Catalog # :

MA1-070

Quantity :

100 µg

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Human, Many, Mouse, Rat

Applications :

Flow Cytometry: 1-2 µg/test, Immunocytochemistry: 2-4 µg/mL, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Species :

Bovine, Human, Many, Mouse, Rat

Clone :

74.1

Isotype :

IgG2b

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.

Immunogen :

Purified bovine brain cytoplasmic dynein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1-2 µg/test, Immunocytochemistry: 2-4 µg/mL, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Aliases :

1700064A15Rik; 6720463E02Rik; 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; 8kDLC; C87222; CDLC2; cytoplasmic dynein light polypeptide; cytoplasmic dynein light polypeptide 1; cytoplasmic light-chain dynein; cytosolic DH IC-1; DLC1; Dlc2; DLC8; DLC8b; DNCL1; DNCL1B; Dnclc1; dynein LC8; dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; dynein, light chain, LC8-type 2; DYNLL1; DYNLL2; HDLC1; LC8; LC8; PIN; LC8a; LOC540369 protein; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog; RSPH22; similar to cytoplasmic dynein light chain 1

more info or order :

Invitrogen product webpage