Vimentin Monoclonal Antibody (RV203) | Invitrogen MA1-06908 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Vimentin Monoclonal Antibody (RV203)

catalog :

MA1-06908

quantity :

100 µL

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RV203

reactivity :

domestic goat, Chinese hamsters, human, mouse, rat, dogs, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 7	Santi A, Caselli A, Ranaldi F, Paoli P, Mugnaioni C, Michelucci E, et al. Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth. Biochim Biophys Acta. 2015;1853:3211-23 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Vimentin Monoclonal Antibody (RV203)

Catalog # :

MA1-06908

Quantity :

100 µL

Price :

US 425.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Bovine, Canine, Goat, Hamster, Human, Mouse, Porcine, Rat

Applications :

Flow Cytometry: 1:100-1:200, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1:100-1:2,000

Species :

Bovine, Canine, Goat, Hamster, Human, Mouse, Porcine, Rat

Clone :

RV203

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Immunogen :

Cytoskeletal vimentin extract of calf lens.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100-1:200, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1:100-1:2,000

Aliases :

cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus

more info or order :

Invitrogen product webpage